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1.
Braz. j. microbiol ; 40(2): 238-240, Apr.-June 2009. tab
Article in English | LILACS | ID: lil-520211

ABSTRACT

The partial DNA sequences of the 18S rRNA gene of Babesia canis and the 16S rRNA gene of Ehrlichia canis detected in dogs from Ribeirão Preto, Brazil, were compared to sequences from other strains deposited in GenBank. The E. canis strain circulating in Ribeirão Preto is identical to other strains previously detected in the region, whereas the subspecies Babesia canis vogeli is the main Babesia strain circulating in dogs from Ribeirão Preto.


As sequências parciais dos genes RNAr 18S de Babesia canis e RNAr 16S e Ehrlichiacanis detectados em cães de Ribeirão Preto, Brasil, foram comparadas à sequências de outras linhagens depositadas no GeneBank. A linhagem de E. canis circulando em Ribeirão Preto é idêntica a outras detectadas previamente na região, enquanto a sub-espécie B. canis vogeli é a principal linhagem de Babesia circulando em cães de Ribeirão Preto.


Subject(s)
Animals , Dogs , Babesiosis , Base Sequence , Babesia/genetics , Ehrlichiosis , Ehrlichia canis/genetics , In Vitro Techniques , Polymerase Chain Reaction , RNA , Ticks , Methods , Diagnostic Techniques and Procedures
2.
Braz J Microbiol ; 40(2): 238-40, 2009 Apr.
Article in English | MEDLINE | ID: mdl-24031351

ABSTRACT

The partial DNA sequences of the 18S rRNA gene of Babesia canis and the 16S rRNA gene of Ehrlichia canis detected in dogs from Ribeirão Preto, Brazil, were compared to sequences from other strains deposited in GenBank. The E. canis strain circulating in Ribeirão Preto is identical to other strains previously detected in the region, whereas the subspecies Babesia canis vogeli is the main Babesia strain circulating in dogs from Ribeirão Preto.

3.
Braz. j. microbiol ; 38(3): 478-479, July-Sept. 2007. tab
Article in English | LILACS | ID: lil-464774

ABSTRACT

Comparison of the partial DNA sequence of the 16S rRNA gene of Anaplasma platys detected in dogs from Ribeirão Preto, Brazil, to sequences of other strains previously deposited in GenBank showed that there are at least three A. platys strains circulating in South America.


A comparação de sequências parciais do gene 16S RNAr de Anaplasma platys detectadas em cães de Ribeirão Preto, Brasil, com sequências de outras linhagens previamente depositadas no GenBank indicam que existem pelo menos três linhagens de A. platys circulando na América do Sul.


Subject(s)
Dogs , Anaplasma , Anaplasmataceae , In Vitro Techniques , Methods , Polymerase Chain Reaction
5.
Int Surg ; 84(1): 43-5, 1999.
Article in English | MEDLINE | ID: mdl-10421017

ABSTRACT

The authors report a recently examined rare case of isolated perforated diverticulum of the transverse colon. At surgery, a perforated omentum-sealed diverticulum was found in the third distal wall of the transverse colon. A diverticulectomy was performed. The postoperative course was excellent.


Subject(s)
Abdomen, Acute/etiology , Diverticulum, Colon/complications , Diverticulum, Colon/surgery , Aged , Aged, 80 and over , Diverticulum, Colon/diagnosis , Female , Humans , Treatment Outcome
6.
J Exp Clin Cancer Res ; 18(1): 75-7, 1999 Mar.
Article in English | MEDLINE | ID: mdl-10374682

ABSTRACT

The Authors present the preliminary results of a study aimed at verifying the effectiveness of lymphatic mapping for the dissection of the sentinel lymph node in the treatment of breast carcinoma. The lymphoscintigraphy method was used in the study performed on 24 patients to evidence the sentinel lymph node. The sentinel lymph node was identified in 23/24 patients (95,8%), with 100% accuracy, negative predictions were 100% (21/21). Consistently with the low number of cases treated, our results seem to guarantee the same prognostic accuracy obtained using radical lymphadenectomy extended to the third lymph node level.


Subject(s)
Breast Neoplasms/pathology , Breast Neoplasms/surgery , Lymph Node Excision , Lymph Nodes/pathology , Adult , Aged , Biopsy , Breast Neoplasms/diagnostic imaging , Female , Humans , Lymph Nodes/diagnostic imaging , Lymphatic Metastasis , Middle Aged , Neoplasm Staging , Radionuclide Imaging , Reproducibility of Results
7.
Ann Ital Chir ; 69(4): 433-8, 1998.
Article in Italian | MEDLINE | ID: mdl-9835116

ABSTRACT

Still controversial is the role of surgery in symptomatic colon diverticular disease, outside the major complications. In such circumstances the surgeon has to comfort himself with diagnostic and then with operative choices, and also the preventive colectomy has to be considered. To make a correct decision very important is the global evaluation of the patient, for age, sex life habits, other existing pathologies, general condition. Also unsolved doubt for a neoplastic evolution has its worth.


Subject(s)
Colectomy , Diverticulitis, Colonic/prevention & control , Diverticulum, Colon/surgery , Colectomy/methods , Diverticulum, Colon/complications , Elective Surgical Procedures , Emergencies , Female , Humans , Male , Middle Aged
8.
Ann Ital Chir ; 69(1): 97-100, 1998.
Article in English | MEDLINE | ID: mdl-11995044

ABSTRACT

Morgagni-Larrey's hernias are congenital diaphragmatic hernias, rarely observed and generally identified accidentally due to the lack of symptoms. The authors report a case of intestinal occlusion secondary to Morgagni-Larrey herniation in an adult recently observed and underline the aetiopathogenic aspects, the diagnostic tools and the clinical pattern. They suggest to consider surgical repair as a choice of treatment, also for asymptomatic patients.


Subject(s)
Hernia, Diaphragmatic/complications , Hernias, Diaphragmatic, Congenital , Intestinal Obstruction/etiology , Adult , Humans , Male
10.
Jpn Heart J ; 38(1): 67-72, 1997 Jan.
Article in English | MEDLINE | ID: mdl-9186282

ABSTRACT

From 1982 to 1994, 54 patients (47 men; mean age 72 years) were referred to the Hospital of Ferrara, Italy for spontaneous rupture of abdominal aortic aneurysm. Sixteen died in the emergency department and 38 underwent urgent surgery. Day and month of onset of acute symptoms leading to urgent surgery were recorded. A seasonal variation with significant peaks in spring and autumn was found. These findings are likely influenced by local environmental, social and epidemiological factors, but may be relevant for the appropriate timing of the follow-up and therapeutic strategies for abdominal aortic aneurysms.


Subject(s)
Aortic Aneurysm, Abdominal/etiology , Aortic Rupture/etiology , Seasons , Aged , Analysis of Variance , Aortic Aneurysm, Abdominal/epidemiology , Aortic Aneurysm, Abdominal/surgery , Aortic Rupture/epidemiology , Aortic Rupture/surgery , Circadian Rhythm , Emergencies , Female , Humans , Male
11.
J Mal Vasc ; 22(2): 128-36, 1997 May.
Article in English | MEDLINE | ID: mdl-9243334

ABSTRACT

OBJECTIVE: A prospective study was performed in order to compare results obtained in the treatment of early and/or limited primary varicose veins of the lower limbs using two different procedures: external valvuloplasty and high ligation or disconnection of the sapheno-femoral junction. MATERIALS AND METHODS: 116 limbs (113 patients) were selected. 57 with normal cusps in dilated valves were subjected to external valvuloplasty with Silicone prosthesis under Doppler control (intraoperative angioscopy in 16 cases); 59 limbs were subjected to high ligation or disconnection of the junction; 57 limbs out of 116 were subjected to complementary procedures. Duplex and photoplethysmographic examinations were performed before and after the surgical procedures in all patients. Doppler venous pressures were measured in 36 limbs and invasive pressures in 40 limbs. Patients were postoperatively followed up every 4 months until the 12th month. RESULTS: Indications for valvuloplasty were found in 8.2% of cases and in 66.3% of the early varices. Clinical results were slightly superior in the reparative surgery group. Thrombotic occlusion of the proximal long saphenous vein was significantly higher in the ligation-disconnection group. Results from photoplethysmography and venous pressure measurements indicated that both operations are equally effective in the elimination of reflux in the junction.


Subject(s)
Catheterization , Venous Insufficiency/surgery , Adult , Blood Vessel Prosthesis , Female , Humans , Ligation , Male , Middle Aged , Photoplethysmography , Prospective Studies , Ultrasonography, Doppler
12.
Minerva Cardioangiol ; 44(9): 437-42, 1996 Sep.
Article in Italian | MEDLINE | ID: mdl-8999368

ABSTRACT

The authors describe their experience concerning the surgical treatment of aneurysmatic pathology of the popliteal artery. This is the most frequent site for aneurysm after aortic aneurysm. Since 1979 to 1994, 26 patients, 21 men and 5 women (mean age 65.8 years), have been treated at the Institute of Clinical Surgery of the University of Ferrara for a total of 37 popliteal aneurysms. Thirteen patients had more than one aneurysm. Before the operation, all patients underwent DSA, and echo Doppler, 7 with CAT and 4 with RMN. Twenty-nine substitutions, 7 by passes and 1 ligation were performed. Twelve of the 29 substitutions were made by reversed saphenous vein. Fourteen by PTFEe, and 3 by dacron. In 4 cases it was necessary to reimplant either the anterior tibial artery (3 cases) or the posterior tibial artery (1 case) separately. The results obtained were good especially in the post-trauma patients. Only 3 amputations were necessary due to graft obstruction immediately after surgery, and in post-traumatic conditions.


Subject(s)
Aneurysm/surgery , Popliteal Artery , Aged , Aneurysm/diagnosis , Aneurysm/diagnostic imaging , Angiography, Digital Subtraction , Blood Vessel Prosthesis , Female , Follow-Up Studies , Humans , Magnetic Resonance Imaging , Male , Polyethylene Terephthalates , Polytetrafluoroethylene , Saphenous Vein/transplantation , Time Factors , Tomography, X-Ray Computed , Ultrasonography, Doppler
13.
Minerva Cardioangiol ; 44(9): 447-55, 1996 Sep.
Article in Italian | MEDLINE | ID: mdl-8999369

ABSTRACT

HYPOTHESIS: To create a phlebolymphologic therapy in order: to activate venous system; to activate lymphatic system; to activate macrophagic system; to reduce the proteic lymphatic load. EXPERIMENTAL: A study was performed on the use of an ideal phlebolymphological association (Tonka Beans, Gingko Biloba, Melilotus Officinalis) as a practical standpoint in the treatment of lymphedema of lower limbs in order to create an efficacious dose of Coumarin, Benzopyrones and Ginkolidi. CLINICAL: We investigated a population of 76 patients treated in an open-label study for six-eight months with a dosage of Coumarin 60 mg/daily + Gingko Biloba 40 mg/daily + Melilotus 40 mg/daily. CONCLUSION: This trilogy induced a very significant improvement in lymphedema (centimeter-aspect) both in functional symptoms (pain heaviness in affected limbs) and physical signs (edema, episodes of infection). Tolerance of long term treatment was good and the improvement was observed from the third month of treatment.


Subject(s)
Anticoagulants/administration & dosage , Coumarins/administration & dosage , Leg , Lymphedema/drug therapy , Phytotherapy , Plant Extracts/administration & dosage , Adolescent , Adult , Aged , Drainage , Drug Therapy, Combination , Follow-Up Studies , Humans , Lymphedema/classification , Lymphedema/surgery , Middle Aged , Time Factors
14.
J Cardiovasc Surg (Torino) ; 37(4): 345-51, 1996 Aug.
Article in English | MEDLINE | ID: mdl-8698777

ABSTRACT

The presence of proteins (albumin and globulins) in lymphedematous tissue not only gives rise to colloidosmotic pressure but also produces an electrostatic charge endowing the proteins with individual features and different migration rates. The working hypothesis of the experimental study is to transfer lymph proteins from the upper fascia accumulation area to a subfascial drainage area by subjecting them to an adequate difference in potential. A double chamber, variable volume system with separation wall able to contain a 1 cm square of muscle fascia, was designed and built; the aim of the apparatus was to reproduce the subcutaneus zone separated by the fascia interposition, from the muscle-vascular zone. At the system was applied a variable electric field in six different experiments: 4 using porous synthetic membranes and 2 using human muscle fascia.


Subject(s)
Fascia/metabolism , Lymph/metabolism , Lymphedema/metabolism , Proteins/metabolism , Biological Transport , Electrophysiology , Humans , In Vitro Techniques , Leg , Membranes, Artificial , Models, Structural , Porosity
15.
Minerva Chir ; 51(6): 443-6, 1996 Jun.
Article in Italian | MEDLINE | ID: mdl-8992393

ABSTRACT

Postoperative infections are an outstanding problem in a surgical department. We have been studying them from a clinical and experimental point of view has a long time. In this study we present a method standardisation of postoperative peritoneal lavage as prevention of surgical infections. Winstar mice, infected with intra peritoneal E. coli cultures, underwent peritoneal lavage with saline solution or sterile water. The results showed the right amount of solution and the exact administration of lavage according to grade of infection. Encouraged by this result we are going to test the efficacy of anti microbial agents for the postoperative peritoneal lavage.


Subject(s)
Peritoneal Lavage/standards , Postoperative Care/methods , Animals , Male , Mice
16.
Ann Ital Chir ; 67(2): 225-7; discussion 227-8, 1996.
Article in Italian | MEDLINE | ID: mdl-8929038

ABSTRACT

The spontaneous pneumothorax (pnx. s.) is a type of pathology with a high per cent of relapse so its aetiological treatment can't be done only with a pleural drainage. With the resection of the bubble (that is the cause of it) instead, the problem will be resolved without possibilities of relapses. In the past, this was possible only with a thoracotomy, now with the new techniques of video-thoracoscopy, the therapeutical approach to this pathology has been revolutionized. In fact, this methodology resolves definitively the pulmonary lesion, can avoid the relapses, can guarantee a short stay in hospital and a very good restarting of the working and social activity.


Subject(s)
Endoscopy , Pneumothorax/surgery , Thoracoscopy/methods , Adult , Aged , Follow-Up Studies , Humans , Male , Middle Aged , Recurrence , Time Factors , Video Recording
17.
Surg Endosc ; 10(1): 60-1, 1996 Jan.
Article in English | MEDLINE | ID: mdl-8711609

ABSTRACT

A case of peritoneal cystic mesothelioma in a young woman was treated with a minimally invasive approach. The technique and a review of the literature are presented.


Subject(s)
Laparoscopy , Mesothelioma, Cystic/surgery , Minimally Invasive Surgical Procedures , Peritoneal Neoplasms/surgery , Adult , Diagnostic Imaging , Female , Follow-Up Studies , Humans , Mesothelioma, Cystic/diagnosis , Peritoneal Neoplasms/diagnosis
18.
Minerva Chir ; 50(11): 973-7, 1995 Nov.
Article in Italian | MEDLINE | ID: mdl-8710151

ABSTRACT

The most frequent cause of spontaneous pneumothorax is bleb's disease of the lung. Considering that, pnx s. has a remarkable bent for relapsing, we think that it's necessary an aggressive treatment to resolve it. That's because pleural drainage is useful for a symptomatic resolution, VATS for an etiologycal one. To value the real efficacy of the treatment of VATS, we have effected a retrospective research between two classes of patients suffering from pnx s. admitted in our Institute from 1987 until 1991, one treated with pleural drainage (class A, 11 patients) and the other with VATS (class B, 13 patients). 1) Patients treated with pleural drainage were nearly double compared to class B. 2) In class A, the mean value of drainages were nearly double compared to class B. 3) Class A had a number of relapses 10 times more compared to B. 4) Considering the period of hospitalization, class A had a value nearly triple compared to B. 5) We've noticed that the mean value of the cost for episode of pnx s. in class A was more than 15% compared to class B and the cost for patient in class A was nearly double than in class B. We can affirm that, for the treatment of pnx s., the method of VATS rappresents a real success. We're passed from the "symptomatological treatment" of it to an aetiological one, joined to the prophylaxis of the relapse.


Subject(s)
Drainage , Pleura , Pneumothorax/surgery , Pneumothorax/therapy , Thoracotomy/methods , Videotape Recording , Humans , Pneumothorax/economics , Pneumothorax/prevention & control , Recurrence , Retrospective Studies
19.
Minerva Cardioangiol ; 43(6): 247-56, 1995 Jun.
Article in Italian | MEDLINE | ID: mdl-7566537

ABSTRACT

Lower limb critical ischemia is a clinical condition typical of patients with severe chronic obstructive arterial disease (Fontaine's IIIb and IV degree). This condition often leads to amputation of the limb involved. The authors present to the use PGE1 in 50 patients with Fontaine's IIIb-IV degree chronic obstructive arterial disease of lower limbs in which the indication of amputation was done. All the patients, admitted to the emergency ward, complain of rest pain and distal ulcers. The administration of PG5(1) was given as follows: 40 mg/bid/e.v./20 days. A 6 months long follow-up was installed with the instrumental evaluation of: Transcutaneous oxygen pressure; Distal blood perfusion with Doppler; cardiac pulse; blood pressure. Eighteen patients became to a Fontaine's II degree during the next 2 months after therapy, 25 patients came back to a severe claudicatio: of them 18 underwent successfully vascular surgery, 7 underwent amputation of the lower limb. In 7 patients the PGE1 did not influence the natural progression of the disease. Among the side-effects of therapy we can mention: headache (4%), erythema and pain of injected vein (8%), sick (4%). All the side effects were transient and never led to interruption of therapy.


Subject(s)
Alprostadil/therapeutic use , Ischemia/drug therapy , Leg/blood supply , Aged , Alprostadil/adverse effects , Amputation, Surgical , Arterial Occlusive Diseases/drug therapy , Arterial Occlusive Diseases/surgery , Chronic Disease , Female , Humans , Ischemia/surgery , Leg/surgery , Male , Middle Aged
20.
G Chir ; 16(5): 251-2, 1995 May.
Article in Italian | MEDLINE | ID: mdl-7654505

ABSTRACT

The experience with video-assisted mediastinoscopy in 16 cases examined, is reported. Initially the classic mediastinoscopic device with the aid of an operative thoracoscopy was used. Starting from April 1994 the Authors used the video-assisted mediastinoscopy which, enlarging the surgical field, allows to improve the operative performance and may be a good aid for multiple teaching.


Subject(s)
Mediastinoscopes , Video Recording , Humans , Thoracoscopes
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