Comparative analysis of greywater pollutant removal efficiency with horizontal free water surface flow wetland with other wetland technologies.

The reuse of treated wastewater for agriculture and other purposes is globally recognized as a reliable water source. Constructed wetlands are cost-effective and reliable green technologies for wastewater treatment, offering an environmentally friendly and affordable solution with minimal operational and maintenance requirements. This study assessed four wetland technologies (HFWSF, VFSF, VSSF, and HSSF) for treating greywater according to regulatory standards. The technologies effectively maintained pH levels, and both treated and untreated greywater samples met FEPA limits. They efficiently reduced dissolved and suspended particles, remaining below FEPA discharge limits for conductivity, TDS, turbidity, and TSS. However, elevated ammonia levels in both treated and untreated samples required additional treatment or mitigation. Sulphate levels were successfully mitigated, and phosphorus limits were met, with HFWSF already compliant even before treatment. Nitrate levels were reduced to meet FEPA limits, ensuring regulatory compliance. While BOD limits were met in both treated and untreated samples, untreated samples exceeded COD limits, necessitating more efficient treatment methods. HFWSF and HSSF complied with COD limits, whereas VFSF and VSSF did not. Both treated and untreated samples exceeded FEPA limits for oil and grease, indicating the need for additional treatment. Untreated samples exhibited high coliform contamination levels, underscoring the importance of effective treatment. However, all technologies successfully reduced coliform levels in treated samples, meeting FEPA limits and confirming treatment effectiveness. The combination of Typha (Domingensis) in the horizontal subsurface flow constructed wetland improved pollutant removal, nutrient removal, and contaminant elimination. Incorporating water Hyacinth (Eichhornia crassipes) with horizontal free water surface flow wetland technology demonstrated the highest efficacy in removing various pollutants. This combination outperformed other wetland technologies in effectively removing pollutants, including ammonia (60%), oil and grease (78.46%), COD (85%), TP (37.04%), FC (75%), and TC (79.59%), representing significant progress in greywater treatment.

How Much Will Safe Sanitation for all Cost? Evidence from Five Cities.

Global sustainable development goals call for universal access to safely managed sanitation by 2030. Here, we demonstrate methods to estimate the financial requirements for meeting this commitment in urban settings of low-income countries. Our methods considered two financial requirements: (i) the subsidies needed to bridge the gap between the willingness-to-pay of low-income households and actual market prices of toilets and emptying services and (ii) the amounts needed to expand the municipal waste management infrastructure for unserved populations. We applied our methods in five cities- Kisumu, Malindi, Nakuru in Kenya; Kumasi in Ghana; and Rangpur in Bangladesh and compared three to five sanitation approaches in each city. We collected detailed cost data on the sanitation infrastructure, products, and services from 76 key informants across the five cities, and we surveyed a total of 2381 low-income households to estimate willingness-to-pay. We found that the total financial requirements for achieving universal sanitation in the next 10 years and their breakdown between household subsidies and municipal infrastructure varied greatly between sanitation approaches. Across our study cities, sewerage was the costliest approach (total financial requirements of 16-24 USD/person/year), followed by container-based sanitation (10-17 USD/person/year), onsite sanitation (2-14 USD/person/year), and mini-sewers connecting several toilets to communal septic tanks (3-5 USD/person/year). Further applications of our methods can guide sanitation planning in other cities.

Clean vehicles, polluted waters: empirical estimates of water consumption and pollution loads of the carwash industry.

Carwash stations use large volumes of water and release harmful chemicals into the environment through their operations. While a significant body of literature has focused on exploring water use in the carwash industry, none has provided comprehensive information on both the pollution loads of the wastewater emanating from this industry and water consumption. Understanding how much water is used and the pollution loads of wastewater from this industry is useful to ensure adoption of water conservation measures and design wastewater recycling systems given the dwindling freshwater resources globally. This study estimated the freshwater quantities used to wash different vehicle types and the pollution loads of the resulting wastewater in the Kumasi Metropolis. Seven proxy carwash stations were purposively selected and monitored to estimate the water used to wash six different categories of vehicles. Composite wastewater samples from three carwash stations were analysed for concentrations of different contaminants which were used to compute pollution loads. Using R software, one-way ANOVA with Tukey's (HSD) post-hoc testing and 2-sample t-test at 95% confidence interval were employed to test statistical differences. After an 8-week monitoring campaign involving 3,667 vehicles, the study showed that average water used for each vehicle type were in the order: Motorbike - 97L (95% CI: 90-103L); Salon car - 158L (95% CI: 154-161L); SUV - 197L (95% CI:191-203L); Buses/Coaches - 370L (95%CI:351-381L); Articulated truck 1,139L (95% CI:916-1,363L); Graders/Loaders - 1405L (95% CI:327-2,483L). Overall, the carwash industry in the Metropolis uses about 1000m3 of freshwater daily and discharges the resulting wastewater into waterways untreated. The wastewater has a low Biodegradability Index (0.3-0.4) and is characterized by a mildly alkali pH (7.6-8.6) with high levels of Sulphates (40.8-69.8 mg/L), COD (990-1413 mg/L), TSS (1260-3417 mg/L) and E. coli (2.3-4.7 × 103 CFU/100mL). Pollution loads of BOD and COD were up to 2tons/year and 6tons/year respectively. Stipulated effluent discharge guideline values were mostly exceeded - in some cases by up to 68 times. To avert the unbridled wastage of freshwater, the study recommends enforcement of wastewater recycling for all carwash stations and promulgation of a tax system that rewards stations that recycle wastewater and surchages those wasting freshwater.

Treating waste with waste: the potential of synthesized alum from bauxite waste for treating car wash wastewater for reuse.

This study assessed the contaminant removal potential of a low-cost alum synthesized from bauxite slime waste compared to industrial grade alum [Al2(SO4)3.18H2O] in treating car wash wastewater using standard jar tests. The synthesized alum was subsequently applied as a coagulant to test the short-term performance of a bench scale flocculation-flotation system for treating car wash wastewater. Coagulant dosages and mixing intensities were optimized for both coagulants and differences were analyzed with R using two-way ANOVA with Tukey's (HSD) post hoc testing. Per the jar tests, percentage removal of up to 99%, 34%, and 75% of turbidity, anionic surfactants (AS), and COD, respectively, was achieved with 90 mg/L of the synthesized alum compared to 100%, 37%, and 74% for industrial grade alum. Contaminant removal efficiencies of both coagulants were comparable (p > 0.05). However, coagulant dosage strongly influenced the removal of turbidity, AS, and COD (p < 0.05) while mixing intensity influenced all but COD. The bench-scale flocculation-flotation system completely removed turbidity (100%) and reduced AS and COD by up to 92% and 99% respectively. The results of this study demonstrate the potential of alum synthesized from bauxite slime waste as a cheaper alternative for industrial grade alum in wastewater recycling for the car wash industry.

Novel Bioengineered Cassava Expressing an Archaeal Starch Degradation System and a Bacterial ADP-Glucose Pyrophosphorylase for Starch Self-Digestibility and Yield Increase.

To address national and global low-carbon fuel targets, there is great interest in alternative plant species such as cassava (Manihot esculenta), which are high-yielding, resilient, and are easily converted to fuels using the existing technology. In this study the genes encoding hyperthermophilic archaeal starch-hydrolyzing enzymes, α-amylase and amylopullulanase from Pyrococcus furiosus and glucoamylase from Sulfolobus solfataricus, together with the gene encoding a modified ADP-glucose pyrophosphorylase (glgC) from Escherichia coli, were simultaneously expressed in cassava roots to enhance starch accumulation and its subsequent hydrolysis to sugar. A total of 13 multigene expressing transgenic lines were generated and characterized phenotypically and genotypically. Gene expression analysis using quantitative RT-PCR showed that the microbial genes are expressed in the transgenic roots. Multigene-expressing transgenic lines produced up to 60% more storage root yield than the non-transgenic control, likely due to glgC expression. Total protein extracted from the transgenic roots showed up to 10-fold higher starch-degrading activity in vitro than the protein extracted from the non-transgenic control. Interestingly, transgenic tubers released threefold more glucose than the non-transgenic control when incubated at 85°C for 21-h without exogenous application of thermostable enzymes, suggesting that the archaeal enzymes produced in planta maintain their activity and thermostability.