ABSTRACT
The European legislation on cosmetic products has recently required the declaration of 26 compounds (24 volatile chemicals and 2 natural extracts) on the label of final products when exceeding a stipulated cut-off level. In this work a rapid reliable and specific RP-HPLC method coupled with diode array detector (DAD) has been developed for the simultaneous determination and quantification of the 24 volatile chemicals: amyl cinnamal, benzyl alcohol, cinnamyl alcohol, citral, eugenol, hydroxy-citronellal, isoeugenol, amylcinnamyl alcohol, benzyl salicylate, cinnamal, coumarin, geraniol, Lyral (hydroxy-methylpentylcyclohexene carboxaldehyde), anisyl alcohol, benzyl cinnamate, farnesol, Lilial (2-(4-tert-butylbenzyl)propionaldehyde) linalool, benzyl benzoate, citronellol, hexyl cinnamal, limonene, methylheptin carbonate, alpha-isomethyl ionone (3-methyl-4-(2,6,6-trimethyl-2-cyclohexen-1-yl)-3-buten-2-one). The 24 analytes were appropriately separated over a running time of 40 min, on a C18 column using a simple gradient elution (acetonitrile/water) with flow rate from 0.7 to 1.0 ml/min and UV acquisition at 210, 254 and 280 nm. All calibration curves showed good linearity (r2>0.99) within test ranges. The method was successfully applied to the qualitative and quantitative determination of the potential allergens in four commercial scented products, with satisfactory accuracy and precision. The results indicated that this simple and efficient method can be used for quality assessment of complex matrices such us cosmetic scented products.
Subject(s)
Allergens/analysis , Chromatography, High Pressure Liquid/methods , Perfume/chemistry , Allergens/chemistry , Calibration , Chromatography, High Pressure Liquid/instrumentation , Molecular Structure , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
Ethylene glycol monoethyl ether or 2-ethoxyethanol finds a wide industrial application as a solvent for lacquers, inks, dyes, household products and as a surfactant. It is also found in cosmetics such as nail products, face cleansers, liquid soaps, oral care products, hair colours and fixatives. The potential hazard to human health of 2-ethoxyethanol following inhalation and dermal exposure has been recently reviewed and the European Cosmetic, Toiletry and Perfumery Association (COLIPA) has issued recommendations suggesting its non-use as a cosmetic ingredient. Therefore a simple and fast monitoring method is necessary for routine control to identify and quantify 2-ethoxyethanol in raw materials and finished cosmetics. We have developed a sensitive and selective method to determine 2-ethoxyethanol in complex matrices by precolumn derivatization with 1-naphthyl isocyanate and RP-HPLC analysis. Four laboratory-made cosmetic formulations (a nail lacquer remover, a baby oil, a skin lotion and an emollient O/W emulsion) containing three known amounts of 2-ethoxyethanol (0.1%, 2.0%, 5.0%) have been studied. The obtained results show that this chromatographic procedure provides a good estimate of the true concentration of 2-ethoxyethanol in complex matrices and it is reliable for routine analyses in quality control.
ABSTRACT
The synthesis of nine quaternary ammonium iodides derived from omega-dialkylaminoethyl ethers of 5-(arylmethylene)-1,3, 3-trimethyl-2-oxabicyclo[2.2.2]octan-6-hydroxyimines, as potential cosmetic ingredients, is described. They are routinely prepared starting from cineole aminoethers by reaction with iodoethane and their physics-chemical data are reported. These substances were studied for their UV absorption and three of these compounds were also submitted to microbiological assays on five test organisms. The substances have their UV absorption maxima at 284-321 nm, and one compound is active on Staphylococcus aureus, Streptococcus faecalis and Candida albicans. These preliminary findings seem to indicate that some of these compounds could be considered as potential UV sunscreens; one compound, having a moderate antimicrobial activity, could be considered a potential active compound in cosmetics as deodorants, toothpastes, mouthwashes and other oral care products.
Subject(s)
Bridged Bicyclo Compounds, Heterocyclic/chemistry , Cosmetics/analysis , Anti-Infective Agents/chemical synthesis , Anti-Infective Agents/pharmacology , Sunscreening Agents/chemical synthesis , Sunscreening Agents/pharmacologyABSTRACT
Synopsis Materials and methods for the synthesis of eight quaternary ammonium bromides of 5-[4-(omega-dialkylaminoalkoxy)phenylmethylene]-1,3,-trimethyl-2-oxabicyclo[2.2.2]octan-6-ones are illustrated. They were routinely prepared starting from cineole aminoethers by reaction with primary alkyl bromides and their physico-chemical data are reported. These substances have been tested for UV filtering and/or microbiological activity. The substances have their UV absorption maxima at 315-322 nm. Tests on antimicrobial activity were performed using benzalkonium chloride as reference standard. All quaternary ammonium bromides were totally inactive against Escherichia coli (Gram -) and partially active on Staphylococcus aureus (Gram +). These preliminary findings seem to indicate that these new quaternary ammonium bromides could be considered as potential UV sunscreens.
ABSTRACT
Synopsis Semipermanent or direct colouring includes any product capable of affecting to some extent a change in the natural hair colour that will last through at least five shampoo washings. Semipermanent dyes are simple and easy to use, as opposed to oxidation dyes, and are normally formulated for application on nonbleached hair. Following increases in supply of such formulations, we have started an analysis for quality control purposes of 21 commonly marketed dyestuffs (nitroaminobenzenes, anthraquinone and Arianor dyes) and 20 colouring products manufactured by four leading companies. By using TLC (silica gel and reversed phase) and HPTLC (silica gel) procedures we have determined relative retention values to 1,3-diamino-4-nitrobenzene of standards and dyes found in the commercial products. All the values reported (standards and samples) are the average of five analytical results (+/-SD).
ABSTRACT
A series of 5-(arylmethylene)-1,3,3-trimethyl-2-oxabicyclo[2.2.2]octan-6- ones was prepared by reaction of (+)-1,3,3-trimethyl-2-oxabicyclo[2.2.2]octan-6-one with aromatic aldehydes in an alkaline medium. These compounds can be considered as potential UVB or UVA sunscreens. In vitro phototoxicity tests (photohemolysis and Candida albicans) showed that they exhibit in general a low phototoxicity level.
