Subject(s)
Down Syndrome/genetics , Pregnancy Complications , Abortion, Induced , Adolescent , Adult , Female , Humans , Pregnancy , Pregnancy in Adolescence , Prenatal DiagnosisSubject(s)
Prenatal Diagnosis , Abortion, Spontaneous , Adult , Chorionic Villi/analysis , Female , Humans , Karyotyping , Male , Maternal Age , Middle Aged , Pregnancy , Pregnancy Trimester, FirstSubject(s)
Chromosomes, Human, Pair 18 , Prenatal Diagnosis , Trisomy , Abnormalities, Multiple/diagnosis , Abnormalities, Multiple/genetics , Abortion, Therapeutic , Adult , Chromosome Aberrations/diagnosis , Chromosome Disorders , Female , Humans , Maternal Age , Phenotype , Pregnancy , Pregnancy Trimester, SecondABSTRACT
Two pregnancies at risk for Fanconi's anemia have been monitored by a cytogenetic method in the first trimester of gestation. The rate of chromosome breakage was evaluated in spontaneous mitoses from a direct preparation of trophoblasts in one case and from mitoses obtained from standard cultures and from mitoses treated with diepoxybutane in both cases. Cytogenetic studies were carried out also at 16 weeks from amniotic fluid cells in one case, and in fibroblasts sampled from the aborted fetus in the other. In all the experimental conditions the mean frequency of breaks/cell was in the range of controls, suggesting that the fetuses were unaffected by Fanconi's anemia. In one case the results have been confirmed by chromosome analysis at birth.
Subject(s)
Anemia, Aplastic/diagnosis , Chorionic Villi/ultrastructure , Chromosome Aberrations , Fanconi Anemia/diagnosis , Prenatal Diagnosis/methods , Adult , Fanconi Anemia/genetics , Female , Gestational Age , Humans , Karyotyping , PregnancySubject(s)
Chromosome Aberrations , Chromosome Disorders , Prenatal Diagnosis , Abortion, Habitual/genetics , Abortion, Spontaneous/genetics , Chromosome Aberrations/diagnosis , Chromosome Inversion , Female , Genetic Counseling , Humans , Infant, Newborn , Karyotyping , Male , Pregnancy , Risk , Translocation, Genetic , TrisomyABSTRACT
We have considered the possibility of using commercial Blasto-Kit, with AB pool, for chromosome analysis. Comparative researches have been made between Blasto-Kit-AB and Blasto-Kit in which AB serum was replaced by FCS. Using 0.1 ml of whole blood lymphocyte response to PHA after incorporation of thymidine H3 and the mitotic rate obtained have been evaluated. The Blasto-Kit-AB gives better results if compared with Blasto-Kit-FCS either in lymphocyte response to PHA or in chromosome analysis.
Subject(s)
Karyotyping , ABO Blood-Group System , Animals , Cattle , Female , Fetus/immunology , Humans , Lymphocyte Activation , Pregnancy , Reagent Kits, DiagnosticABSTRACT
The authors propose a rapid method for chromosome studies in human abortions as a supplementary or alternative method to embryonic tissue cultures. Cultures were set up from fetal lymphocytes and thymus and liver cells. 72 hours was the longest time necessary to obtain suitable chromosome preparations. A very high rate of success was obtained in the 28 cases studied.
