Effect of perfluorochemical emulsion on lipoproteins of plasma blood.

The effect of perfluorocarbons (PFC, Perfluorodecalin/Fogalin) on the lipoprotein level in plasma was studied. The PFC emulsion (FCE) was injected i.v. at a dose of 6 g PFC per kg of body weight into male Wistar rats weighing 250-280 g. The lipoproteins were separated by preparative centrifugation. It was found that PFCs influence the metabolism of lipoproteins. The greatest depletion of cholesterol level occurs in the HDL fraction of lipoproteins 3 and 24 hrs after injection. There is no relationship between the level of cholesterol in plasma and cholesterol lipoproteins. The content in lipoproteins, however, amounts to half the level of the cholesterol of plasma 72 hrs after injection. Based on these facts it is possible to conclude that when PFCs leave the cells of organs, they are carmed in the blood stream as a complex which contains at least PFCs, cholesterol and triglycerides.

Some aspects of perfluorochemical emulsion's interaction with blood.

FDC/FTPA (7:3) emulsions stabilized by procsanol (Emulsion 1) and by procsanol with yolk phospholipids (Emulsion 2) were incubated with the donor plasma. After the incubation during 6 hours of Emulsions 1 and 2 with plasma the 36% and 50% decrease of the cholesterol content in plasma was found. Analysis of the lipid content of lipoproteins after the Emulsion 2 administration to rats (2.5 ml/100 g of weight) revealed the 50% decrease of the cholesterol amount in the HDL fraction at 3 and 24 hours posttransfusion. The ratio cholesterol/total phospholipids in the erythrocyte membrane diminished up to 50% as well. The equal degree of the cholesterol adsorption by emulsion from plasma, HDL of rats and erythrocyte membrane is an evidence of nonspecific interaction of PFC particles with the blood components containing cholesterol.

[Changes in lactate dehydrogenase activity and lactic and pyruvic acid concentration in the blood and tissues of animals following intravenous administration of perfluorotributylamine emulsions]. / Izmenenie aktivnosti laktatdegidrogenazy i soderzhaniia molochnoi i pirovinogradnoi kislot v krovi i tkaniakh zhivotnykh posle vnutrivennogo vvedeniia émul'sii perftortributilamina.

Activity of lactate dehydrogenase (LDH) and concentration of lactate and pyruvate were studied in rat and cat tissues after infusion of perfluorotributylamine emulsion. Administration of the emulsion caused an increase in content of lactate and pyruvate as well as LDH activation in the animal blood and tissues. Tissue hypoxia, responsible for stimulation of glycolytic pathway of carbohydrate metabolism, was apparently developed due to transfer of inadequate amount of oxygen by perfluorotributylamine.