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1.
Phys Rev Lett ; 116(9): 098001, 2016 Mar 04.
Article in English | MEDLINE | ID: mdl-26991202

ABSTRACT

A thermodynamically equilibrated fluid of hard spheroids is a simple model of liquid matter. In this model, the coupling between the rotational degrees of freedom of the constituent particles and their translations may be switched off by a continuous deformation of a spheroid of aspect ratio t into a sphere (t=1). We demonstrate, by experiments, theory, and computer simulations, that dramatic nonanalytic changes in structure and thermodynamics of the fluids take place, as the coupling between rotations and translations is made to vanish. This nonanalyticity, reminiscent of a second-order liquid-liquid phase transition, is not a trivial consequence of the shape of an individual particle. Rather, free volume considerations relate the observed transition to a similar nonanalyticity at t=1 in structural properties of jammed granular ellipsoids. This observation suggests a deep connection to exist between the physics of jamming and the thermodynamics of simple fluids.

2.
Phys Rev E Stat Nonlin Soft Matter Phys ; 86(2 Pt 1): 021404, 2012 Aug.
Article in English | MEDLINE | ID: mdl-23005760

ABSTRACT

In materials science the phase-field crystal approach has become popular to model crystallization processes. Phase-field crystal models are in essence Landau-Ginzburg-type models, which should be derivable from the underlying microscopic description of the system in question. We present a study on classical density functional theory in three stages of approximation leading to a specific phase-field crystal model, and we discuss the limits of applicability of the models that result from these approximations. As a test system we have chosen the three-dimensional suspension of monodisperse hard spheres. The levels of density functional theory that we discuss are fundamental measure theory, a second-order Taylor expansion thereof, and a minimal phase-field crystal model. We have computed coexistence densities, vacancy concentrations in the crystalline phase, interfacial tensions, and interfacial order parameter profiles, and we compare these quantities to simulation results. We also suggest a procedure to fit the free parameters of the phase-field crystal model. Thereby it turns out that the order parameter of the phase-field crystal model is more consistent with a smeared density field (shifted and rescaled) than with the shifted and rescaled density itself. In brief, we conclude that fundamental measure theory is very accurate and can serve as a benchmark for the other theories. Taylor expansion strongly affects free energies, surface tensions, and vacancy concentrations. Furthermore it is phenomenologically misleading to interpret the phase-field crystal model as stemming directly from Taylor-expanded density functional theory.

3.
Osteoarthritis Cartilage ; 19(11): 1356-62, 2011 Nov.
Article in English | MEDLINE | ID: mdl-21872669

ABSTRACT

OBJECTIVES: (1) assess the molecular weight dependence of hyaluronan's (HA) cartilage boundary lubricating ability, alone and in combination with proteoglycan 4 (PRG4), at physiological concentrations; (2) determine if HA and PRG4 interact in solution via electrophoretic mobility shift assay (EMSA). METHODS: The cartilage boundary lubricating ability of a broad range of MW HA (20 kDa, 132 kDa, 780 kDa, 1.5 MDa, and 5 MDa) at 3.33 mg/ml, both alone and in combination with PRG4 at 450 µg/ml, was assessed using a previously described cartilage-on-cartilage friction test. Static, µ(static, Neq), and kinetic, <µ(kinetic, Neq)>, were calculated. An EMSA was conducted with PRG4 and monodisperse 150 kDa and 1,000 kDa HA. RESULTS: Friction coefficients were reduced by HA, in a MW-dependent manner. Values of <µ(kinetic, Neq)> in 20 kDa HA, 0.098 (0.089, 0.108), were significantly greater compared to both 780 kDa, 0.080 (0.072, 0.088), and 5 MDa, 0.079 (0.070, 0.089). Linear regression showed a significant correlation between both µ(static, Neq) and <µ(kinetic, Neq)>, and log HA MW. Friction coefficients were also reduced by PRG4, and with subsequent addition of HA; however the synergistic effect was not dependent on HA MW. Values of <µ(kinetic, Neq)> in PRG4, 0.080 (0.047, 0.113), were significantly greater than values of PRG4+various MW HA (similar in value, averaging 0.040 (0.033, 0.047)). EMSA indicated that migration of 150 kDa and 1,000 kDa HA was retarded when combined with PRG4 at high PRG4:HA ratios. CONCLUSIONS: These results suggest alterations in HA MW could significantly affect synovial fluid's cartilage boundary lubricating ability, yet this diminishment in function could be circumvented by physiological levels of PRG4 forming a complex, potentially in solution, with HA.


Subject(s)
Cartilage, Articular/drug effects , Friction/drug effects , Hyaluronic Acid/pharmacology , Proteoglycans/pharmacology , Viscosupplements/pharmacology , Animals , Cattle , Drug Therapy, Combination , Electrophoretic Mobility Shift Assay , Molecular Weight , Stifle/drug effects
4.
J Phys Condens Matter ; 23(19): 194120, 2011 May 18.
Article in English | MEDLINE | ID: mdl-21525557

ABSTRACT

The crystallization of a metastable melt is one of the most important non-equilibrium phenomena in condensed matter physics, and hard sphere colloidal model systems have been used for several decades to investigate this process by experimental observation and computer simulation. Nevertheless, there is still an unexplained discrepancy between the simulation data and experimental nucleation rate densities. In this paper we examine the nucleation process in hard spheres using molecular dynamics and Monte Carlo simulation. We show that the crystallization process is mediated by precursors of low orientational bond-order and that our simulation data fairly match the experimental data sets.


Subject(s)
Crystallization , Molecular Dynamics Simulation , Monte Carlo Method , Suspensions/chemistry , Computer Simulation , Particle Size , Thermodynamics
5.
Phys Rev E Stat Nonlin Soft Matter Phys ; 81(4 Pt 1): 042101, 2010 Apr.
Article in English | MEDLINE | ID: mdl-20481772

ABSTRACT

Motivated by biological aspects related to fungus growth, we consider the competition of growth and corrosion. We study a modification of the totally asymmetric exclusion process, including the probabilities of injection alpha and death of the last particle delta . The system presents a phase transition at deltac(alpha), where the average position of the last particle L grows as sqrt[t]. For delta>deltac, a nonequilibrium stationary state exists while for delta

Subject(s)
Phase Transition , Biological Transport , Diffusion , Fungi/chemistry , Fungi/growth & development , Fungi/metabolism , Models, Biological , Time Factors
6.
J Neurol Neurosurg Psychiatry ; 67(2): 174-9, 1999 Aug.
Article in English | MEDLINE | ID: mdl-10406984

ABSTRACT

OBJECTIVES: To determine the molecular basis for autosomal dominant intermediate hereditary motor and sensory neuropathy (HMSN) in a four generation family. The gene defects in families with intermediate HMSN are not known, but it has been suggested that most have X linked HMSN. METHODS: All participating family members were examined clinically. Genomic DNA was obtained from 10 affected and seven unaffected members. Linkage analysis for the known HMSN loci was first performed. Mutations in the peripheral myelin protein zero gene (PMP0) were sought in two affected members, using one unaffected member for comparison, by amplification of the six exons of the gene followed by single strand conformation polymorphism (SSCP) analysis, dideoxy fingerprinting (ddF), and sequencing. Subsequently, the mutation was screened for in all affected and unaffected members in the family using Alu I digestion and in 100 unrelated control subjects using "snap back" SSCP analysis. Sequencing of cDNA from a sural nerve biopsy from an affected member was also performed. RESULTS: The clinical phenotype was of variable severity, with motor nerve conduction velocities in the intermediate range. Linkage to PMP0 was demonstrated. Analysis of genomic DNA and cDNA for PMP0 identified a novel codon 35 GAC to TAC mutation. The mutation produces an inferred amino acid change of aspartate to tyrosine at codon six of the processed protein (Asp6Tyr) in the extracellular domain and was present in all affected family members but not in 100 unrelated controls. CONCLUSIONS: The present findings further extend the range of phenotypes associated with PMP0 mutations and indicate that families with "intermediate" HMSN need not necessarily be X-linked as previously suggested.


Subject(s)
Hereditary Sensory and Motor Neuropathy/genetics , Mutation/physiology , Myelin P0 Protein/genetics , Adult , Aged , Base Sequence/genetics , Biopsy , DNA/genetics , DNA, Complementary/genetics , Female , Genetic Linkage/genetics , Genome , Heterozygote , Humans , Male , Middle Aged , Molecular Sequence Data , Pedigree , Sural Nerve/pathology
7.
Cytogenet Cell Genet ; 72(4): 294-6, 1996.
Article in English | MEDLINE | ID: mdl-8641132

ABSTRACT

Sequence-tagged sites (STSs) were developed for the human alpha-tropomyosin gene TPM4. One STS was used to amplify DNA from somatic cell hybrids to localize TPM4 to chromosome 19. The other, a product from a long-range PCR, was used directly as a probe to refine the localization of TPM4 to 19p13.1 by fluorescence in situ hybridization to metaphase chromosome spreads.


Subject(s)
Chromosomes, Human, Pair 19/genetics , Tropomyosin/genetics , Animals , Base Sequence , Chromosome Mapping , DNA Primers/genetics , DNA Probes/genetics , Humans , Hybrid Cells , In Situ Hybridization, Fluorescence , Molecular Sequence Data , Polymerase Chain Reaction , Sequence Tagged Sites
9.
Am J Hum Genet ; 56(2): 422-7, 1995 Feb.
Article in English | MEDLINE | ID: mdl-7847377

ABSTRACT

We have studied a family segregating a form of autosomal dominant distal myopathy (MIM 160500) and containing nine living affected individuals. The myopathy in this family is closest in clinical phenotype to that first described by Gowers in 1902. A search for linkage was conducted using microsatellite, VNTR, and RFLP markers. In total, 92 markers on all 22 autosomes were run. Positive linkage was obtained with 14 of 15 markers tested on chromosome 14, with little indication of linkage elsewhere in the genome. Maximum two-point LOD scores of 2.60 at recombination fraction .00 were obtained for the markers MYH7 and D14S64--the family structure precludes a two-point LOD score > or = 3. Recombinations with D14S72 and D14S49 indicate that this distal myopathy locus, MPD1, should lie between these markers. A multipoint analysis assuming 100% penetrance and using the markers D14S72, D14S50, MYH7, D14S64, D14S54, and D14S49 gave a LOD score of exactly 3 at MYH7. Analysis at a penetrance of 80% gave a LOD score of 2.8 at this marker. This probable localization of a gene for distal myopathy, MPD1, on chromosome 14 should allow other investigators studying distal myopathy families to test this region for linkage in other types of the disease, to confirm linkage or to demonstrate the likely genetic heterogeneity.


Subject(s)
Chromosomes, Human, Pair 14 , Genes, Dominant , Muscular Diseases/genetics , Adolescent , Adult , Child , Child, Preschool , DNA, Satellite , Female , Genetic Linkage , Genetic Markers , Humans , Male , Muscles/pathology , Pedigree , Polymerase Chain Reaction
10.
Nat Genet ; 9(1): 75-9, 1995 Jan.
Article in English | MEDLINE | ID: mdl-7704029

ABSTRACT

Nemaline myopathies are diseases characterized by the presence in muscle fibres of pathognomonic rod bodies. These are composed largely of alpha-actinin and actin. We have identified a missense mutation in the alpha-tropomyosin gene, TPM3, which segregates completely with the disease in a family whose autosomal dominant nemaline myopathy we had previously localized to chromosome 1p13-q25. The mutation substitutes an arginine residue for a highly conserved methionine in a putative actin-binding site near the N terminus of the alpha-tropomyosin. The mutation may strengthen tropomyosin - actin binding, leading to rod body formation, by adding a further basic residue to the postulated actin-binding motif.


Subject(s)
Myopathies, Nemaline/genetics , Point Mutation , Tropomyosin/genetics , Amino Acid Sequence , Base Sequence , Chromosomes, Human, Pair 1 , DNA/genetics , DNA Mutational Analysis , DNA Primers/genetics , Exons , Female , Genes, Dominant , Genetic Linkage , Humans , Male , Molecular Sequence Data , Pedigree , Polymorphism, Genetic
11.
Cytogenet Cell Genet ; 71(1): 94-5, 1995.
Article in English | MEDLINE | ID: mdl-7606936

ABSTRACT

A sequence tagged site (STS) was developed for the human beta tropomyosin gene (TPM2). The STS was used to amplify DNA from somatic cell hybrids to localise TPM2 to human chromosome 9. Genomic clones isolated with the STS product were in turn used in fluorescence in situ hybridisation to metaphase chromosome spreads to further localise TPM2 to 9p13.


Subject(s)
Chromosomes, Human, Pair 9 , Tropomyosin/genetics , Animals , Base Sequence , Chromosome Mapping , DNA Primers , Humans , Hybrid Cells , In Situ Hybridization, Fluorescence , Male , Mice , Molecular Sequence Data , Sequence Tagged Sites
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