ABSTRACT
The work presents the results of experimental transplantation of a not-divided embryonic myocardium culture into the mesentery of the adult rats' intestine for the terms of about 30 days. The influence of Cimitidine--cardiolen on the development of this culture was investigated. The grafts were shown to be taken and to function well and the use of cardiolen reliably improves the development of the culture.
Subject(s)
Heart Transplantation , Heart/embryology , Adjuvants, Immunologic/pharmacology , Animals , Cimetidine/pharmacology , Culture Media , Culture Techniques , Data Interpretation, Statistical , Mesentery , Rats , Rats, Wistar , Time FactorsABSTRACT
Some natural peptides, referred to as cytomedines, were isolated from different organs: cortexin and epithalamin (both from the brain), cordialin (heart), hepalin (liver) and thymalin (thymus),--to test their stimulating effects on the growth in organotypic culture of different tissue explants taken from 3 day old rats. It has been found that these peptides exerted their obvious stimulating effects on the growth of the cultured explants, compared to the control, if taken in the respective concentrations: 100, 50, 50, 100 and 5 ng/ml. Thus, these cytomedins can be used in the clinical practice for stimulating reparative processes in the appropriate tissues.