ABSTRACT
Pampus argenteus is a species of economic importance for professional fishing and aquaculture. To analyse the quality of oocytes of P. argenteus, 10 females were kept in tanks of 200 m2 with an average depth of 1 m in the Integrated Center of Fishing Resources and Aquaculture of Três Marias - CODEVASF. Specimens were submitted to hypophysation-induced reproduction with crude extract of common carp pituitary (EBHC). Females received two doses (0.8-1.0 and 5.0-6.6 mg of EBHC/kg body weight, respectively), with a 12-h interval between doses. For males, a single dose (2.7 mg/kg body weight) was applied at the same time as the females' second dose. Oocytes were extruded manually 8 h after the second hormonal dose at 26°C. It was observed that seven females responded positively to the procedure while the other three released bloody and lumpy oocytes. For histological analysis, ovarian fragments were fixed in Bouin's liquid for 8-12 h and submitted to routine histological techniques. The protocol was considered successful for 70% of females and 100% of males. The fertilization rate of the females from the unsuccessful group was very low, and histological analyses showed that most of their oocytes were in follicular atresia, suggesting a delay in hormonal administration or extrusion could have occurred. Despite the hypophysation protocol being considered adequate for the induced reproduction of P. argenteus, complementary studies are necessary to evaluate the possible causes of this degenerative process.
Subject(s)
Follicular Atresia , Ovary , Animals , Body Weight , Female , Male , Oocytes , ReproductionABSTRACT
Our study aimed to establish the response of Salminus franciscanus to hypophysation and describe the main morphological events of its embryonic process. Wild fish were captured in São Francisco River and selected broodstock (females: 66.4 ± 11.1 cm and 4.04 ± 2.32 kg; males: 58.3 ± 10.2 cm and 3.62 ± 1.12 kg) were kept at 26.1 ± 0.6°C for induction of final maturation/gamete release via the hypophysation technique. In females, two doses (0.8 and 5.6 mg/kg body weight) of crude pituitary extract of common carp (Cyprinus carpio) were administered with a 14 h interval. For males, a single dose (2.7 mg/kg body weight) of crude pituitary extract was applied at the same time as the females' second dose. Oocytes and sperm were manually stripped 8 h after a females' second hormonal dose. Fertilization was carried out using the dry method. Eggs were kept in funnel-type 60 L incubators at 24.3 ± 0.3°C and were analyzed and photographed every 10 min. After hormonal induction, 60% of females and 100% of males reacted positively and no broodstock mortality was recorded. The females released an average of 385.2 ± 78.4 g of oocytes and the fertilization rate observed was 50.4 ± 12.3%. The blastopore closure occurred at 7.5 h, somite formation at 12 h and hatching at 20 h post-fertilization. In general, the results of this study improve the understanding of the reproductive biology of dourado and confirm its potential for fish farming in the neotropical region.
Subject(s)
Carps , Characiformes , Animals , Female , Male , Oocytes , Reproduction , RiversABSTRACT
The objective of this work was to evaluate the fatty acid and lipid composition of oocytes, newly hatched larvae (NHL), first feeding larvae (FFL) and muscle tissue of female Brycon orthotaenia broodstock. Total and polar lipid was significantly (P<0.05) abundant in oocytes and larvae in different stages of development. The lowest content (P<0.05) of total lipids was found in the muscles, whereas total lipid content of oocytes, NHL and FFL did not show any significant difference. Polar lipid content was different (P<0.05) between NHL and FFL. For the neutral the lowest values of C18:2n 6 occurred during the initial feeding period, whilst C20:4n 6 (AA) exhibited the highest percentage in FFL (P<0.05). C22:6n 3 (DHA) was highest (P<0.05) in FFL. The neutral lipid n-9 and n-6 was highest in muscle of females. The n-3HUFA was highest in NHL and in FFL, n-6HUFA was highest in FFL (P<0.05). The ratios of DHA/EPA were higher (P<0.05) in oocytes and FFL. In fatty acids of polar lipids, C20:5n 3 (EPA) did not show differences (P>0.05) between stages. C18:3n 3 was highest (P<0.05) in NHL and FFL. C20:4n 6 (AA) and C22:6n 3 (DHA) showed the highest percentages during the larval stages. The fatty acids n-3 series was significantly higher (P<0.05) in FFL. The n-6HUFA was highest during development larval (P<0.05). The increases DHA reflects the ability of the species to elongate and desaturate to obtain n-3HUFA from 18:3n 3, shows the importance of this fatty acid during early development.
