ABSTRACT
Biocatalysis is a green and sustainable technology for which the ideal solvent should be nontoxic, biocompatible, biodegradable, and sustainable, in addition to supporting high enzyme activity and stability. Deep eutectic solvents (DESs), a novel class of green solvents, have recently emerged as excellent alternatives for use in various biocatalytic reactions and, in particular, in lipase-catalyzed reactions with enzymes. This review discusses the achievements that have been made so far in the use of DESs as reaction media for lipase-catalyzed reactions. In addition, the application of DESs in esterification, transesterification, and amidation reactions with isolated or immobilized biocatalysts, toward enabling the synthesis of biodiesels, sugar esters, phenolipids, and fatty acyl ethanolamides, is summarized, while advances in lipase-catalyzed chemoenzymatic epoxidation reactions, C-C bond-forming Aldol reactions, and hydrolysis reactions in DESs are also discussed. This review also summarize some remaining questions concerning the use of DESs, including the intriguing role of water as a cosolvent in biocatalytic reactions carried out in DESs, and the relationship between the nature of the DESs and their influence on the enzyme stability and activity at the molecular level.
Subject(s)
Biocatalysis , Enzymes, Immobilized/metabolism , Lipase/metabolism , Solvents/chemistry , Green Chemistry Technology/trends , Hydrogen Bonding , Organic Chemistry PhenomenaABSTRACT
In this study, deep eutectic solvents (DESs) were used for the ultrasound-assisted extraction (UAE) of valuable bioactive compounds from Chinese wild rice (Zizania spp.). To this end, 7 different choline chloride (CC)-based DESs were tested as green extraction solvents. Choline chloride/1,4-butanediol (DES-2) exhibited the best extraction efficiency in terms of parameters such as the total flavonoid content (TFC), total phenolic content (TPC), and free radical scavenging capacity (DPPHâ and ABTSâ+). Subsequently, the UAE procedure using 76.6% DES-2 was also optimized: An extraction temperature of 51.2 °C and a solid-liquid ratio of 37.0 mg/mL were considered optimal by a Box-Behnken experiment. The optimized extraction procedure proved efficient for the extraction of 9 phenolic and 3 flavonoid compounds from Chinese wild rice as determined by quantification based on ultra-performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-QqQ-MS). This work, thus, demonstrates the possibility of customizing green solvents that offer greater extraction capacity than that of organic solvents.
