Microalgae Harvesting by Self-Driven 3D Microfiltration with Rationally Designed Porous Superabsorbent Polymer (PSAP) Beads.

Microalgae are emerging as next-generation renewable resources for production of sustainable biofuels and high-value bioproducts. Conventional microalgae harvesting methods including centrifugation, filtration, flocculation, and flotation are limited by intensive energy consumption, high capital cost, long treatment time, or the requirement of chemical addition. In this study, we design and fabricate porous superabsorbent polymer (PSAP) beads for self-driven 3D microfiltration of microalgal cultures. The PSAP beads can swell fast in a microalgal suspension with high water absorption capacity. During this process, microalgal cells are excluded outside the beads and successfully concentrated in the residual medium. After treatment, the beads can be easily separated from the microalgal concentrate and reused after dewatering. In one PSAP treatment, a high concentration factor for microalgal cultures up to 13 times can be achieved in 30 min with a harvesting efficiency higher than 90%. Furthermore, microalgal cultures could be concentrated from 0.2 g L-1 to higher than 120 g L-1 with minimal biomass loss through multistage PSAP treatments. Therefore, the use of PSAP beads for microalgae harvesting is fast, effective, and scalable. It does not require any complex instrument or chemical addition. This technique potentially provides an efficient and feasible alternative to obtain high concentrations of functional biomass at a very low cost.

Self-Driven Pretreatment and Room-Temperature Storage of Water Samples for Virus Detection Using Enhanced Porous Superabsorbent Polymer Beads.

The continuous emergence of infectious viral diseases has become a major threat to public health. To quantify viruses, proper handling of water samples is required to ensure the accuracy and reliability of the testing results. In this study, we develop enhanced porous superabsorbent polymer (PSAP) beads to pretreat and store water samples for virus detection. By applying PSAP beads to collect water samples, the viruses are captured and encapsulated inside the beads while undesired components are excluded. We have successfully demonstrated that the shelf life of the model virus can be effectively extended at room temperature (22 °C) and an elevated temperature (35 °C). Both the infectivity level and genome abundance of the viruses are preserved even in a complex medium such as untreated wastewater. Under the tested conditions, the viral degradation rate constant can be reduced to more than 10 times using the PSAP beads. Therefore, the enhanced PSAP beads provide a low-cost and efficient sample pretreatment and storage method that is feasible and practical for large-scale surveillance of viral pathogens in water samples.

Self-Driven "Microfiltration" Enabled by Porous Superabsorbent Polymer (PSAP) Beads for Biofluid Specimen Processing and Storage.

A remote collection of biofluid specimens such as blood and urine remains a great challenge due to the requirement of continuous refrigeration. Without proper temperature regulation, the rapid degradation of analytical targets in the specimen may compromise the accuracy and reliability of the testing results. In this study, we develop porous superabsorbent polymer (PSAP) beads for fast and self-driven "microfiltration" of biofluid samples. This treatment effectively separates small analytical targets (e.g., glucose, catalase, and bacteriophage) and large undesired components (e.g., bacteria and blood cells) in the biofluids by capturing the former inside and excluding the latter outside the PSAP beads. We have successfully demonstrated that this treatment can reduce sample volume, self-aliquot the liquid sample, avoid microbial contamination, separate plasma from blood cells, stabilize target species inside the beads, and enable long-term storage at room temperature. Potential practical applications of this technology can provide an alternative sample collection and storage approach for medically underserved areas.

Bioelectrochemically assisted anaerobic digestion system for biogas upgrading and enhanced methane production.

The objective of this study was to evaluate the effect of biofilm and external voltage on the performance and microbial community composition of batch-fed, combined anaerobic digestion-bioelectrochemical cell (AD-BEC) systems under different operational conditions. A dextrin/peptone mixture was fed at a range of organic loading rates (0.34 to 1.37g COD/L-d). The hybrid system with both suspended biomass and biofilm without any external potential application achieved a substantially higher initial soluble COD consumption (53.7±2.3% vs. 39.7±3.7) and methane (CH4) production (331 vs. 225mL) within one day of feeding than the conventional AD system (suspended biomass only). Compared to the conventional AD system, the hybrid systems had higher resilience to shock organic loadings. A range of external potential (0.5 to 2.0V vs. Ag/AgCl) was applied to AD-BEC reactors, developed with two different start-up procedures. A potential of 2.0V resulted in water electrolysis leading to a higher CH4 production rate (105 vs. 84mL/L-d) and biogas CH4 content (88.5±1.4 vs. 64.5±1.9%) in the AD-BEC reactor (closed vs. open circuit condition, respectively). Application of external potential enriched putative exoelectrogens at the anode biofilm and hydrogenotrophic methanogens at the cathode biofilm, which may have contributed to the observed enhanced CH4 production in the AD-BEC system. A phylotype related to Methanobacterium formicicum, a hydrogenotrophic methanogen, dominated the archaeal community in the AD-BEC cathode biofilm.

Microbial synthesis of Pd/Fe3O4, Au/Fe3O4 and PdAu/Fe3O4 nanocomposites for catalytic reduction of nitroaromatic compounds.

Magnetically recoverable noble metal nanoparticles are promising catalysts for chemical reactions. However, the chemical synthesis of these nanocatalysts generally causes environmental concern due to usage of toxic chemicals under extreme conditions. Here, Pd/Fe3O4, Au/Fe3O4 and PdAu/Fe3O4 nanocomposites are biosynthesized under ambient and physiological conditions by Shewanella oneidensis MR-1. Microbial cells firstly transform akaganeite into magnetite, which then serves as support for the further synthesis of Pd, Au and PdAu nanoparticles from respective precursor salts. Surface-bound cellular components and exopolysaccharides not only function as shape-directing agent to convert some Fe3O4 nanoparticles to nanorods, but also participate in the formation of PdAu alloy nanoparticles on magnetite. All these three kinds of magnetic nanocomposites can catalyze the reduction of 4-nitrophenol and some other nitroaromatic compounds by NaBH4. PdAu/Fe3O4 demonstrates higher catalytic activity than Pd/Fe3O4 and Au/Fe3O4. Moreover, the magnetic nanocomposites can be easily recovered through magnetic decantation after catalysis reaction. PdAu/Fe3O4 can be reused in at least eight successive cycles of 4-nitrophenol reduction. The biosynthesis approach presented here does not require harmful agents or rigorous conditions and thus provides facile and environmentally benign choice for the preparation of magnetic noble metal nanocatalysts.