Is the co-application of self-produced compost and natural zeolite interesting to reduce environmental and toxicological availability in metal-contaminated kitchen garden soils?

Composting can turn organic waste into a valuable soil amendment that can improve physical, chemical, and biological soil quality. Compost amendments can also contribute to the remediation of areas anthropogenically degraded by metals. However, it is well known that compost, particularly self-produced compost, can show enrichment in metals. An experimental study was conducted to examine the short- and long-term distribution and the mobility of metals in soils amended with a self-produced compost when it was added alone or in combination with different doses of a natural zeolite to soil. The aim was also to study the interest of managing moderately metal-contaminated kitchen garden soils by assessing the chemical extractability, phytoavailability, and oral bioaccessibility of metals. When zeolite was added to compost alone, it had the tendency to better reduce extractability of Cd and Zn at 25%, and those of Pb at 15%. When the self-produced compost alone or in co-application with zeolite at these doses was applied to soils, the results showed (1) a decrease of NH4NO3-extractable Zn; (2) a reduction of Pb environmental availability, but not Pb bioaccessibility, and (3) an increase of ryegrass biomass. Nevertheless, the risk posed by the self-produced compost was minimal when applied at the proper rate (0.6% w/w). In the selected experimental conditions, the study recommends that self-produced compost be mixed with 15% zeolite to maximize vegetal biomass and minimize environmental risk. The question of sustainability of the results with repeated compost addition is also raised.

Response of Three Miscanthus × giganteus Cultivars to Toxic Elements Stress: Part 2, Comparison between Two Growing Seasons.

The positive impact on restoring soil functionality, decreasing toxic elements (TE) bioaccessibility, and enhancing soil physicochemical and biological parameters established a consensus on considering a Miscanthus × giganteus convenient species for phytomanaging wide TE contaminated areas. Nevertheless, information about the plant's mode of reaction to elevated soil multi-TE concentrations is still scarce. For the sake of investigating the miscanthus response to stressful TE concentrations, an ex-situ pot experiment was initiated for 18 months, with three miscanthus cultivars referred to as B, U, and A planted in soils with gradient Cd, Pb, and Zn concentrations. A non-contaminated control soil was introduced as well, and plants were cultivated within. Results revealed that the long exposure to increasing soil TE concentrations caused the number of tillers per plant to decline and the TE concentrations in the leaves to boost progressively with the soil contamination. The photosynthetic pigments (chlorophyll a, b, and carotenoids) were negatively affected as well. However, the phenolic compounds, flavonoids, tannins, and anthocyanins, along with the antioxidant enzymatic activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase elevated progressively with the TE concentration and exposure duration. Conclusively, miscanthus plants demonstrated an intensified and synchronized antioxidative activity against the TE concentration.

Response of Three Miscanthus × giganteus Cultivars to Toxic Elements Stress: Part 1, Plant Defence Mechanisms.

Miscanthus × giganteus demonstrated good phytostabilization potentials in toxic element (TE) contaminated soils. However, information about its tolerance to elevated concentrations is still scarce. Therefore, an ex-situ pot experiment was launched using three cultivars (termed B, U, and A) grown in soils with a gradient Cd, Pb and Zn concentrations. Control plants were also cultivated in non-contaminated soil. Results show that the number of tillers per plant, stem diameter as well as leaf photosynthetic pigments (chlorophyll a, b and carotenoids) were negatively impacted by soil contamination. On the other hand, phenolic compounds, flavonoids, tannins, and anthocyanins levels along with the antioxidant enzymatic activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase increased in the plants grown on contaminated soils. Altogether, these data demonstrate that miscanthus is impacted by concentrations of toxic elements yet is able to tolerate high levels of soil contamination. These results may contribute to clarifying the miscanthus tolerance strategy against high contamination levels and its efficiency in phytoremediation.

Potentials of Miscanthus x giganteus for phytostabilization of trace element-contaminated soils: Ex situ experiment.

Phytomanagement is proposed as a cost-effective and environmentally-friendly suggestion for sustainable use of large metal-contaminated areas. In the current work, the energy crop miscanthus (Miscanthus × giganteus) was grown in ex situ conditions on agricultural soils presenting a Cd, Pb and Zn contamination gradient. After 93 days of culture, shoot and root growth parameters were measured. Soils and plants were sampled as well to study the TE accumulation in miscanthus and the effects of this plant on TE mobility in soils. Results demonstrated that miscanthus growth depended more on the soils silt content rather than TE-contamination level. Moreover, soil organic carbon at T93 increased in the soils after miscanthus cultivation by 25.5-45.3%, whereas CaCl2-extractible TEs decreased due to complex rhizosphere processes driving plant mineral uptake, and organic carbon inputs into the rhizosphere. In the contaminated soils, miscanthus accumulated Cd, Pb and Zn mainly in roots (BCF in roots: Cd " Zn > Pb), while strongly reducing the transfer of these elements from soil to all organs and from roots to rhizomes, stems and leaves (average TFs: 0.01-0.06, 0.11-1.15 and 0.09-0.79 corresponding to Cd, Pb and Zn respectively). Therefore, miscanthus could be considered a TE-excluder, hence a potential candidate crop for coupling phytostabilization and biomass production on the studied Metaleurop TE-contaminated soils.

Urban kitchen gardens: Effect of the soil contamination and parameters on the trace element accumulation in vegetables - A review.

Trace element contaminants in kitchen garden soils can contribute to human exposure through the consumption of homegrown vegetables. In urban areas, these soils can be contaminated to various degrees by trace element (TE). They are characterized by a great variability in their physicochemical parameters due to the high anthropization level, the wide variety and combination of disturbance sources, as well as the diversity of cultivation practices and the large range of contamination levels. Pollutants can be taken up by vegetables cultivated in these soils and be concentrated in their edible parts. In this review, the behavior of vegetables cultivated in contaminated kitchen gardens is assessed through six examples of the most widely cultivated vegetables (lettuce, tomato, bean, carrot, radish, potato). The role of soil parameters that could influence the uptake of As, Cd, Cr, Ni, Pb, and Zn by these vegetables is also discussed.

Miscanthus x giganteus culture on soils highly contaminated by metals: Modelling leaf decomposition impact on metal mobility and bioavailability in the soil-plant system.

Miscanthus x giganteus is suggested as a good candidate for phytostabilization of metal-polluted soils. Its late harvest in winter generates large amounts of leaf litter on the soil surface. However, little is known about the mobility and the bioavailability of metals following leaf decomposition and the consequences on the succeeding culture. Ex situ artificial aging for 1, 3, and 6 months was conducted with miscanthus leaf fragments incorporated into three agricultural soils displaying a gradient concentration in Cd (0.6, 3.1 and 7.9 mg kg-1), Pb (32.0, 194.6 and 468.6 mg kg-1), and Zn (48.4, 276.3 and 490.2 mg kg-1) to simulate the leaf litter input over 20 years of miscanthus culture. We investigated the impacts on physicochemical and biological soil parameters, CaCl2-extractable metal, and their subsequent ryegrass shoot concentrations, and hence on ryegrass health. The results showed that the amended soils possessed higher pH along with greater available phosphorous and soil organic carbon values. The respiratory activity and microbial biomass carbon in the amended soils increased mainly after 1 month of aging, and decreased afterwards. Despite the higher Pb- and Zn-CaCl2 extractability in the amended soils, the phytoavailability slightly increased only in the most contaminated soils. Moreover, leaf incorporation did not affect the ryegrass biomass, photosynthetic pigment contents, nor the antioxidative enzyme activities. Conclusively, leaf incorporation induced slight variations in soil physicochemical and biological parameters, as well as metal extractability, but not to an extent that might cause a considerable threat to the subsequent culture. Nevertheless, these results are preliminary data that require confirmation by long-term in-situ experimentations as they reflect the modelization of long-term impact of leaf decomposition on soil-plant system.

Evaluation of single-extraction methods to estimate the oral bioaccessibility of metal(loid)s in soils.

Incidental ingestion of polluted soil particles exposes the population to toxic metal(loid)s. To refine the methods of exposure and risk assessment, it is relevant to use bioaccessible concentrations of metal(loid)s determined via in vitro digestion methods. However, some validated methods are complex and costly, involving high technical skills and numerous reagents. The objective of the present study was to evaluate the suitability of four simple chemical extractions to mimic the bioaccessible fraction of As, Cd, and Pb in the gastric (G) and gastrointestinal (GI) phases obtained using the validated UBM (unified bioaccessibility method) test. Acetic acid (0.11 M), citric acid (0.11 M), EDTA (0.16 M), and hydrochloric acid (HCl, 0.65%) were separately tested in 201 soil samples with a wide range of physicochemical parameters and metal(loid)s concentrations. Significant linear relationships were observed with HCl, EDTA, and to a lesser extent with citric acid. For the cheaper HCl method, correlations with the UBM ranged from 0.91 to 0.99 for the G phase and from 0.72 to 0.97 for the GI phase. This test can be used at least as a first-tier screening to assess the oral bioaccessibility of As, Cd, and Pb.

Physiological and histopathological responses of Porcellio laevis (Isopoda, Crustacea) as indicators of metal trace element contamination.

This study was designed to assess the impact of the mixture of cadmium (Cd) and zinc (Zn) on the bioaccumulation and the ultrastructural changes in the hepatopancreas of Porcellio laevis (Latreille, 1804) after 4 weeks of exposure to contaminated Quercus leaves under laboratory conditions. For each metal, four concentrations were used with four replicates for each concentration. Metal concentrations in the hepatopancreas and the rest of the body were determined using atomic absorption spectrometry. From the first week until the end of the experiment, a weight gain in P. laevis was observed particularly between the first and the end of exposure from 93.3 ± 18.22 mg fw to 105.22 ± 16.16 mg fw and from 106.4 ± 22.67 mg fw to 125.9 ± 23.9 mg fw for Mix1 and Mix4, respectively. Additionally, the determined metal trace elements (MTE) concentrations in the hepatopancreas were considerably higher compared to those in the rest of the body and seem to be dose-dependent. Using transmission electron microscopy (TEM), some alterations were highlighted in the hepatopancreas. The main observed alterations were (a) the destruction of the microvilli border in a considerable portion of cells, (b) the increase of the lipid droplets with different shapes and sizes, (c) the increase in the number of the mitochondria, and (d) the appearance of TE in the form of B-type granules. The obtained results confirmed the ability of P. laevis to deal with high amounts of MTE, suggesting its possible use in future soil's biomonitoring programs.

Environmental availability and oral bioaccessibility of Cd and Pb in anthroposols from dredged river sediments.

Dredging and disposal of sediments onto land sites is a common practice in urban and industrial areas that can present environmental and health risks when the sediments contain metallic elements. The aim of this study was to characterise and study the environmental and toxicological availability of Cd and Pb in anthroposols from dredged river sediments. To do this, 67 surface samples spread over 12 sediment disposal sites in northern France were studied. The results showed substantial heterogeneity for this matrix in terms of physicochemical parameters and contamination degree; however, ascending hierarchical clustering made it possible to classify the samples into eight groups. For each group, the mobile fraction of Cd and Pb was studied using single EDTA extraction, solid-phase distribution was analysed with sequential extractions and toxicological availability was assessed with the oral bioaccessibility test. The results showed that (i) Cd had a higher environmental and toxicological availability than Pb; (ii) this availability depends on the physicochemical characteristics of the matrix; and (iii) it is necessary to take into account the environmental and toxicological availability of contaminants when requalifying these sites in order to propose appropriate management measures. In the first years after sediment disposal, it would appear that the environmental and toxicological availability of Cd and Pb increased (from 52.5 to 71.8% and from 28.9 to 48.9%, respectively, by using EDTA and from 50.2 to 68.5% for Cd with the bioaccessibility test). Further studies would therefore be required to confirm this trend and understand the mechanisms involved.

Ex situ evaluation of the effects of biochars on environmental and toxicological availabilities of metals and polycyclic aromatic hydrocarbons.

The present study experimented five biochars, one made from wood (400 °C, 12 h) and four made from miscanthus cultivated on contaminated soils (temperature 400/600 °C, duration 45/90 min). They were used as amendments at a 2% application rate on soil, cultivated or not cultivated with ryegrass, contaminated with (i) metals (Cd, Pb, and Zn), (ii) eight polycyclic aromatic hydrocarbons (PAHs), and (iii) a mix of metals and PAHs. The objectives were (i) to compare the effectiveness of the five biochars on soil parameters and pollutant availability and (ii) to determine the influence of soil multicontamination and ryegrass cultivation on biochar effectiveness. The results showed that biochar application did not necessarily lead to lower pollutant extractability and metal bioaccessibility. However, differences were highlighted between the biochars. The miscanthus biochars produced at 600 °C (BM600) showed higher effectiveness at decreasing metal extractability than the miscanthus biochars produced at 400 °C (BM400) due to its better sorption characteristics. In addition, ryegrass cultivation did not impact pollutant availability but modified metal bioaccessibility, especially for the soil amended with the BM600 and the woody biochar. Moreover, the presence of PAHs also negatively impacted the metal bioaccessibility in the soil amended with the BM600, and, on the contrary, positively impacted it in the soil amended with the BM400. Complementary studies are therefore necessary to understand the mechanisms involved, particularly in a context where soils requiring remediation operations are often multicontaminated and vegetated.

Influence of amendments on metal environmental and toxicological availability in highly contaminated brownfield and agricultural soils.

The immobilizing effects of wood biochar (BW2%) and iron grit (Z1%) applied alone or in combination (BW2% + Z1%) to agricultural (M750) and brownfield (MAZ) soils highly contaminated by metals were assessed in a greenhouse experiment. The results showed that Z1% and BW2% + Z1% were the most efficient amendments to reduce Cd, Cu, Pb, and Zn mobility, environmental availability, and phytoavailability in the M750 soil. The oxidation of Z1% allowed part of the Cu and Zn pools present in exchangeable or carbonate-bound forms (labile fraction) to complex in less mobile forms. In this soil, the metal chemical extractions (0.01 M CaCl2 and 0.05 M EDTA) and the DGT (diffusive gradient in thin films) devices to assess metal in soil solution and soil pore water (SPW) also highlighted the immobilizing characteristic of Z1%. In most cases, the addition of BW2% to Z1% (BW2% + Z1%) did not improve this effect, except for the dissolved Pb and Zn concentrations in the M750 soil solution. It was also observed that Cd, Pb, and Zn passed throughout DGT mimicking the biological cell membrane were reduced by all amendments of the M750 soil corroborating metal concentrations measured in rye grass shoots. In the MAZ soil, metals were less available as shown by their low extractability rate, low capacity of metal resupply from the solid phase to pore water, and low phytoavailability. The poor metal availability could be explained by the high levels of carbonate and organic matter contents in this soil. Nevertheless, a decrease of the Cu environmental availability and the Cu concentrations in rye grass shoots grown on the MAZ soil was also observed in the soil amended with Z1% alone or in combination with BW2%. From a health point of view, the most effective amendment to reduce human exposure through ingestion of soil particles for the M750 and MAZ soils was BW2% for Cd and BW2% + Z1% for Pb. However, the presence of rye grass minimized the amendments' beneficial effects.

Effects of Heavy Metals Artificial Contamination on Porcellio laevis (Latreille, 1804) (Crustacea: Isopoda: Oniscidea).

This study aimed at determining the competition of cadmium (Cd), lead (Pb), zinc (Zn) and copper (Cu) on their assimilation, on the food consumption and the growth of terrestrial isopod Porcellio laevis. Individuals were exposed to artificially contaminated litter of Quercus for 4 weeks and were weekly weighed. At the end of the experiment, the concentration of Cd, Pb, Zn and Cu in individuals were measured by atomic absorption spectrometry. Biological parameters such as growth, and bioaccumulation factor (BAF) were calculated and results from the various treatments were compared. Depending on metals, weight loss or gain were recorded for isopods during the four weeks of exposure. A weight loss was measured on individuals exposed to Cd-contaminated litter whereas a weight gain was highlighted for those exposed to the Zn-contaminated litter. BAF values revealed that P. laevis was macroconcentrator of Zn and Cu and deconcentrator of Cd and Pb.

Bioavailability of mercury in contaminated soils assessed by the diffusive gradient in thin film technique in relation to uptake by Miscanthus × giganteus.

We assessed the relationship between the diffusive gradient in thin film (DGT) technique using the new ion-exchange resin Ambersep GT74 and the uptake of mercury (Hg) by a model plant cultivated on metal-contaminated agricultural soils under greenhouse conditions. Based on the total Hg content, 0.37 to 1.17% of the Hg passed to the soil porewater from the solid phase, and 2.18 to 9.18% of the Hg is DGT-available. These results were confirmed by calculating the R value (the ratio of the concentrations of bioavailable Hg measured by DGT and soil solution), which illustrated the strong bonding of Hg to the solid phase of soil and its extremely low mobility. Only inorganic Hg2+ species were found in the metal-contaminated agricultural soils, as determined by a high-performance liquid chromatography-cold vapor atomic fluorescence spectrometry speciation analysis. The Hg was distributed in Miscanthus × giganteus organs in the following order for all sampling sites: roots (55-82%) >> leaves (8-27%) > stems (7-16%) > rhizomes (4-7%). Environ Toxicol Chem 2019;38:321-328. © 2018 SETAC.

From environmental data acquisition to assessment of gardeners' exposure: feedback in an urban context highly contaminated with metals.

Although growing vegetables in urban gardens has several benefits, some questions in relation with the safety of foods remain when the self-production is carried out on highly contaminated garden soils. To better assess the local population's exposure to Cd and Pb induced by the past activities of a lead smelter, a participatory program was initiated in 115 private kitchen gardens located in northern France to assist gardeners in understanding their soil environment. The challenge included contributing to the database of urban garden soils with the collection of a large number of samples: 1525 crops grouped into 12 types (leaf, fruiting, root, stem and bulbous vegetables, tubers, cabbages, leguminous plants, celeriac, fresh herbs, fruits, and berries), 708 topsoils, and 52 samples of self-produced compost. The main results were as follows: (i) topsoils were strongly contaminated by Cd and Pb compared to regional reference values; (ii) great variability in physicochemical parameters and metal concentrations in topsoils; (iii) the highest concentrations of Cd and Pb for celeriac and fresh herbs and the lowest for fruits and fruiting vegetables; (iv) a high percentage of vegetables that did not comply with the European foodstuff legislation; and (v) most self-produced compost samples were strongly contaminated. This study aimed to raise awareness and generate functional recommendations to reduce human exposure and to provide useful data that could be considered in other environmental contexts.

Measure of environmental stress on Porcellio laevis Latreille, 1804 sampled near active Tunisian industrial areas.

This study aimed to observe the type of asymmetry exhibited by Porcellio laevis sampled from 15 sites belonging to Tunisian industrialized areas. Physicochemical parameters such as pH, organic matter and CaCO3 contents were measured in soils. Moreover, Cd, Pb, Zn, and Cu concentrations were determined in both soils and woodlice. Additionally, 10 metrical traits were measured to evaluate the type of asymmetry on individuals: the basis, the second and the third articles of the antenna, the first article of the flagellum of the antenna and the merus, the carpus, and the propodus of the sixth and the seventh pereopods. Among the 531 measured individuals, 432 exhibited fluctuating asymmetry (FA) while the remaining individuals exhibited antisymmetry or directional asymmetry. The data obtained were analyzed using a multivariate statistical analysis. Contrary to our hypothesis, the results showed that individuals from contaminated sites have a low FA level, whereas those from uncontaminated sites have a high FA level, particularly females but with some exceptions. Variations in FA level in the traits and populations studied and its usefulness as a stress indicator were discussed.

Litter breakdown as a tool for assessment of the efficiency of afforestation and ash-aided phytostabilization on metal-contaminated soils functioning in Northern France.

The main objective of the study was to assess the efficiency of phytoremediation methods implemented for 14 years on highly metal-contaminated soils. The different experimental strategies were plots planted with a tree mix or with a single tree species coupled or not with the use of fly-ashes as an amendment to limit metals mobility in soil. The breakdown of poplar litter on the four plots was monitored during 10 months. In parallel, colonization of litter bags by functional groups of mesofauna (Collembola and Acari) was followed. Two mesh-sized litter bags were used to allow distinguishing microbial and mesofaunal actions on the litter breakdown. We observed the breakdown of litter in four studied plots. Litter breakdown occurred faster in 3-mm litter bags than 250 µm ones during summer demonstrating the importance of mesofauna. Mixed plantation allowed faster litter breakdown than mono-specific plantation. A higher abundance of mesofauna and/or better abiotic conditions (moisture, shading…) could explain this result. Regarding litter breakdown and mesofauna, no significant difference was observed between the amended plots and those subjected to soil phytomanagement. However, communities of the studied area are disturbed since a low abundance of detritivores was observed. This could explain also the slower litter breakdown than expected in our study. To conclude, among the phytomanagement methods tested, mixed plantations could provide a benefit for the restoration of degraded soils. By contrast, the use of fly-ashes does not seem to have any effect on the functionality of ecosystem neither on the litter breakdown process nor on the abundance of mesofauna.

Do biochars influence the availability and human oral bioaccessibility of Cd, Pb, and Zn in a contaminated slightly alkaline soil?

Different remediation techniques have been used to restore metal-contaminated sites, including stabilizing metals by adding amendments to the soils. This study experimented three biochars, made from wood and miscanthus, cultivated on contaminated and uncontaminated soils, used as amendments at a 2% application rate on a metal-contaminated soil for 9 months in laboratory-controlled conditions. The objective was to evaluate whether biochars were able to decrease the availability and human oral bioaccessibility of metals in an alkaline soil. To meet this goal, the modifications of the soil's physicochemical parameters, metal distribution in soil, and human bioaccessibility were evaluated at different sampling times. The results showed that biochar application to the alkaline soil did not always decrease the soil metal availability, which challenges the value of using biochars in already slightly alkaline soils at a low application rate. However, differences in efficiency between the three biochars tested were highlighted. The biochar produced with miscanthus cultivated on uncontaminated soil led to higher soil metal bioaccessibility. Moreover, because of the absence of any increase in soil metal availability with the biochar produced from biomass cultivated on contaminated soil, the use of such biochars can be recommended for the remediation of contaminated soil.

Assessment of oral and lung bioaccessibility of Cd and Pb from smelter-impacted dust.

Soil and dust contamination by metals engenders significant environmental and health problems in northern France where a lead smelter was in activity for more than a century. This study aims to examine the long-term effects of the smelter, 10 years after its closedown, on the presence of metal in sidewalk dust for a better assessment of the local population's exposure to Cd and Pb. The investigation included: (i) the metal distribution in different dust particle sizes and (ii) the assessment of metal bioaccessibility via ingestion and inhalation of dust. Seventy-two sidewalk dust samples were collected using a dust-sampling vacuum. The samples were sieved to collect different particle sizes from 0.3 to 1000 µm. The unified bioaccessibility method (UBM) was employed to evaluate the oral bioaccessibility of metals in the different particle sizes. The pulmonary bioaccessible fraction of Cd and Pb via the finest particles was extracted with lung-simulating solution (artificial lysosomal fluid). Ten years after the smelter closedown, (i) a strong relationship was observed between the concentrations of metals in dust and the distance to the former smelter, whatever the particle size; (ii) both total and oral bioaccessible concentrations of metals were high in the finest fraction (0.3-5 µm) and decreased when the particle size increased; (iii) a higher oral bioaccessibility of Cd and Pb was measured in the gastric phase (on average 43% for both metals for all particle sizes) and compared to the gastrointestinal phase (on average 16% for both metals for all particle sizes); and (iv) metal bioaccessibility via inhalation of dust was relatively high (on average 74 and 69%, for Cd and Pb, respectively). The results of the present study suggest that this environmental compartment may be a sensitive and effective indicator of anthropogenic metal contamination and the human exposure in urban areas.

Value of biochars from Miscanthus x giganteus cultivated on contaminated soils to decrease the availability of metals in multicontaminated aqueous solutions.

The objective of this study was to evaluate the sorption efficiency of eight biochars, made from Miscanthus x giganteus cultivated on contaminated agricultural soil, in aqueous solutions contaminated with metals alone or mixed with polycyclic aromatic hydrocarbons. These biochars were produced in different pyrolysis conditions (temperature, 400/600 °C; heating rate, 5/10 °C min-1; duration, 45/90 min) and compared with an uncontaminated commercialized biochar made of wood. The physicochemical characterization of the Miscanthus biochars confirmed the impact of the pyrolysis on the biochar parameters with substantial differences between the biochars in terms of pH, cation exchange capacity, and specific surface area. The sorption experiment showed higher sorption efficiency of Cd, Pb, and Zn for the Miscanthus biochars produced at 600 °C compared with the biochars produced at 400 °C when the aqueous solutions were mono- or multicontaminated. Furthermore, the desorption study showed that the sorption process was largely irreversible. Therefore, the high sorption capacity of Miscanthus biochars and the low sorption reversibility confirmed that these biochars are a suitable sorbent for metals.

In Vitro Investigations of Human Bioaccessibility from Reference Materials Using Simulated Lung Fluids.

An investigation for assessing pulmonary bioaccessibility of metals from reference materials is presented using simulated lung fluids. The objective of this paper was to contribute to an enhanced understanding of airborne particulate matter and its toxic potential following inhalation. A large set of metallic elements (Ba, Cd, Co, Cr, Cu, Mn, Ni, Pb, Sr, and Zn) was investigated using three lung fluids (phosphate-buffered saline, Gamble's solution and artificial lysosomal fluid) on three standard reference materials representing different types of particle sources. Composition of the leaching solution and four solid-to-liquid (S/L) ratios were tested. The results showed that bioaccessibility was speciation- (i.e., distribution) and element-dependent, with percentages varying from 0.04% for Pb to 86.0% for Cd. The higher extraction of metallic elements was obtained with the artificial lysosomal fluid, in which a relative stability of bioaccessibility was observed in a large range of S/L ratios from 1/1000 to 1/10,000. For further investigations, it is suggested that this method be used to assess lung bioaccessibility of metals from smelter-impacted dusts.