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1.
J Cell Biol ; 163(6): 1339-49, 2003 Dec 22.
Article in English | MEDLINE | ID: mdl-14691141

ABSTRACT

Engagement of integrin receptors with the extracellular matrix induces the formation of focal adhesions (FAs). Dynamic regulation of FAs is necessary for cells to polarize and migrate. Key interactions between FA scaffolding and signaling proteins are dependent on tyrosine phosphorylation. However, the precise role of tyrosine phosphorylation in FA development and maturation is poorly defined. Here, we show that phosphorylation of type Igamma phosphatidylinositol phosphate kinase (PIPKIgamma661) on tyrosine 644 (Y644) is critical for its interaction with talin, and consequently, localization to FAs. PIPKIgamma661 is specifically phosphorylated on Y644 by Src. Phosphorylation is regulated by focal adhesion kinase, which enhances the association between PIPKIgamma661 and Src. The phosphorylation of Y644 results in an approximately 15-fold increase in binding affinity to the talin head domain and blocks beta-integrin binding to talin. This defines a novel phosphotyrosine-binding site on the talin F3 domain and a "molecular switch" for talin binding between PIPKIgamma661 and beta-integrin that may regulate dynamic FA turnover.


Subject(s)
Focal Adhesions/metabolism , Integrin beta Chains/metabolism , Phosphotransferases (Alcohol Group Acceptor)/metabolism , Talin/metabolism , src-Family Kinases/metabolism , Amino Acid Sequence/physiology , Animals , Binding Sites , Cell Membrane/metabolism , Chick Embryo , Focal Adhesion Kinase 1 , Focal Adhesion Protein-Tyrosine Kinases , Humans , Phosphorylation , Protein Binding/physiology , Protein Structure, Tertiary/physiology , Protein-Tyrosine Kinases , Rats , Tyrosine/metabolism
2.
J Biol Chem ; 278(25): 23036-45, 2003 Jun 20.
Article in English | MEDLINE | ID: mdl-12682053

ABSTRACT

Membrane ruffle formation requires remodeling of cortical actin filaments, a process dependent upon the small G-protein Rac. Growth factors stimulate actin remodeling and membrane ruffling by integration of signaling pathways that regulate actin-binding proteins. Phosphatidylinositol 4,5-bisphosphate (PIP2) regulates the activity of many actin-binding proteins and is produced by the type I phosphatidylinositol phosphate kinases (PIPKIs). Here we show in MG-63 cells that only the PIPKIalpha isoform is localized to platelet-derived growth factor (PDGF)-induced membrane ruffles. Further, expression of kinase dead PIPKIalpha, which acts as a dominant negative mutant, blocked membrane ruffling, suggesting that PIPKIalpha and PIP2 participate in ruffling. To explore this, PIPKIalpha was overexpressed in serum-starved cells and stimulated with PDGF. In serum-starved cells, PIPKIalpha expression did not stimulate actin remodeling, but when these cells were stimulated with PDGF, actin rapidly reorganized into foci but not membrane ruffles. PIPKIalpha-mediated formation of actin foci was independent of both Rac1 and phosphatidylinositol 3-kinase activities. Significantly, coexpression of dominant active Rac1 with PIPKIalpha in PDGF-stimulated cells resulted in membrane ruffling. The PDGF- and Rac1-stimulated ruffling was inhibited by expression of kinase-dead PIPKIalpha. Combined, these data support a model where the localized production of PIP2 by PIPKIalpha is necessary for actin remodeling, whereas formation of membrane ruffles required Rac signaling.


Subject(s)
Cell Membrane/ultrastructure , Phosphotransferases (Alcohol Group Acceptor)/metabolism , rac GTP-Binding Proteins/metabolism , Amino Acid Substitution , Base Sequence , Cell Line , Cell Membrane/drug effects , Chromones/pharmacology , Cloning, Molecular , DNA Primers , Enzyme Inhibitors/pharmacology , Fibroblasts/cytology , Fibroblasts/enzymology , Humans , Microscopy, Confocal , Minor Histocompatibility Antigens , Morpholines/pharmacology , Mutagenesis, Site-Directed , Platelet-Derived Growth Factor/pharmacology , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Signal Transduction , rac GTP-Binding Proteins/chemistry
3.
Nature ; 420(6911): 89-93, 2002 Nov 07.
Article in English | MEDLINE | ID: mdl-12422220

ABSTRACT

The ability of cells to form cell contacts, adhere to the extracellular matrix, change morphology, and migrate is essential for development, wound healing, metastasis, cell survival and the immune response. These events depend on the binding of integrin to the extracellular matrix, and assembly of focal adhesions, which are complexes comprising scaffolding and signalling proteins organized by adhesion to the extracellular matrix. Phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P(2)) regulates interactions between these proteins, including the interaction of vinculin with actin and talin. The binding of talin to beta-integrin is strengthened by PtdIns(4,5)P(2), suggesting that the basis of focal adhesion assembly is regulated by this lipid mediator. Here we show that the type I phosphatidylinositol phosphate kinase isoform-gamma 661 (PIPKI gamma 661), an enzyme that makes PtdIns(4,5)P(2), is targeted to focal adhesions by an association with talin. PIPKI gamma 661 is tyrosine phosphorylated by focal adhesion associated kinase signalling, increasing both the activity of phosphatidylinositol phosphate kinase and its association with talin. This defines a mechanism for spatial generation of PtdIns(4,5)P(2) at focal adhesions.


Subject(s)
Focal Adhesions , Phosphotransferases (Alcohol Group Acceptor)/metabolism , Talin/metabolism , Actins/metabolism , Animals , Binding Sites , Cell Line , Cell Membrane/enzymology , Cell Membrane/metabolism , Focal Adhesion Kinase 1 , Focal Adhesion Protein-Tyrosine Kinases , Humans , Integrin beta Chains/metabolism , Isoenzymes/chemistry , Isoenzymes/genetics , Isoenzymes/metabolism , Mice , Phosphatidylinositol 4,5-Diphosphate/metabolism , Phosphotransferases (Alcohol Group Acceptor)/chemistry , Phosphotransferases (Alcohol Group Acceptor)/genetics , Protein Binding , Protein-Tyrosine Kinases/metabolism , Rats , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Talin/chemistry , Vinculin/metabolism
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