Fall armyworm from a maize multi-peril pest risk perspective.

We assembled 3,175 geo-tagged occurrences of fall armyworm worldwide and used that data in conjunction with information about the physiological requirements of the pest to spatially assess its global climate suitability. Our analysis indicates that almost the entire African maize crop is grown in areas with climates that support seasonal infestations of the insect, while almost 92% of the maize area supports year-round growth of the pest. In contrast, rich-country maize production largely occurs in temperate areas where only 2.3% of the area may allow the pest to survive year-round, although still subject to worrisome seasonal risks. This means the African maize crop is especially susceptible to damaging infestation from fall armyworm, on par with the risk exposure to this pest faced by maize producers throughout Latin America. We show that the maize grown in Africa is also especially vulnerable to infestations from a host of other crop pests. Our multi-peril pest risk study reveals that over 95% of the African maize area deemed climate suitable for fall armyworm, can also support year-round survival of at least three or more pests. The spatial concurrence of climatically suitable locations for these pests raises the production risk for farmers well above the risks posed from fall armyworm alone. Starkly, over half (52.5%) of the African maize area deemed suitable for fall armyworm is also at risk from a further nine pests, while over a third (38.1%) of the area is susceptible to an additional 10 pests. This constitutes an exceptionally risky production environment for African maize producers, with substantive and complex implications for developing and implementing crop breeding, biological, chemical and other crop management strategies to help mitigate these multi-peril risks.

Reliability of modified adheremeter and digital pressure algometer in measuring normal abdominal tissue and C-section scars.

OBJECTIVE: This study tested inter- and intrarater reliability of the digital pressure algometer and modified adheremeter and concurrent validity in the algometer in both normal abdominal tissue and in chronically painful C-section scars. STUDY DESIGN: Correlational Reliability/Validity. BACKGROUND: The algometer is used to measure pressure-pain threshold (PPT). The adheremeter is a tool to measure tissue extensibility. Painful abdominal scars are being treated successfully with soft-tissue techniques yet reliable measurement tools for this tissue have not been reported. METHODS AND MEASURES: 59 subjects with normal abdominal tissue were marked at a point 2 inches inferolateral to the umbilicus. Two separate testers measured PPT twice with an algometer and tissue extensibility in superior/left/inferior/right directions with a modified adheremeter. 29 subjects with painful C-section scars were marked at 2.5 cm intervals along the scar. A total of 115 points were measured in the same manner as above. C-section subjects also were asked to rate their pain using the numeric pain rating scale (NPRS). Each tester was blinded to all other measurements. RESULTS: For PPT, intraclass correlations (ICC's) ranged from 0.814 to 0.933 with a standard error of measurement (SEM) ranging from 1.65N to 5.9 N. For tissue mobility, ICC's ranged from 0.430 to 0.914; SEM ranging from 1.67 mm to 3.7 mm. All but 2 measures had ICCs that were good-excellent. Inferior glide in C-section tissues showed the least and multi-directional measurement the strongest reliability. The PPT had a moderate negative correlation (r = -0.551) with the NPRS. CONCLUSIONS: The algometer showed excellent inter- and intra-rater reliability on normal abdominal tissue and C-section scars. It showed moderate criterion validity when compared against the NPRS. The modified adheremeter showed good-excellent inter- and intra-rater reliability on both normal abdominal tissue and C-section scars. Both measures have clinical and research applications for women's health practitioners.

A chromogenic assay of substrate depletion by thiol dioxygenases.

A fast and easy method for enzyme activity assays using the chromogenic Ellman reagent, 5,5'-dithiobis(2-nitrobenzoic acid), was developed. The method was used to measure the activity of the nonheme mono-iron enzyme cysteine dioxygenase. Quantifying the depletion of the substrate, cysteine, allowed standard kinetic parameters to be determined for the enzyme from Rattus norvegicus. The assay was also used to quickly test the effects of ionic strength, pH, enzyme storage conditions, and potential inhibitors and activators. This assay facilitates a higher throughput than available HPLC-based assays, as it enjoys the advantages of fewer sample handling steps, implementation in a 96-well format, and speed. In addition, the relative specificity of Ellman's reagent, coupled with its reaction with a wide range of thiols, means that this assay is applicable to many enzymes. Finally, the use of readily available reagents and instrumentation means that this assay can be used by practically any research group to compare results with those of other groups.