Multistep determination of enzyme activity by flow injection and sequential injection analysis. Assay of amyloglucosidase.

A multi-point assay for determination of the activity of amyloglucosidase (AMG) by FIA and SIA is described. The assay is based on two consecutive reactions that are mutually incompatible. Both the FIA and SIA procedures allow these two reactions to be completely separated, whereby each of the processes can be individually optimized with respect to operational parameters. Special emphasis is placed on comparing the performance and applicability of the two procedures for this type of assay.