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1.
Neuron ; 105(5): 813-821.e6, 2020 03 04.
Article in English | MEDLINE | ID: mdl-31899071

ABSTRACT

Despite being an autosomal dominant disorder caused by a known coding mutation in the gene HTT, Huntington's disease (HD) patients with similar trinucleotide repeat mutations can have an age of onset that varies by decades. One likely contributing factor is the genetic heterogeneity of patients that might modify their vulnerability to disease. We report that although the heterozygous depletion of the autophagy adaptor protein Alfy/Wdfy3 has no consequence in control mice, it significantly accelerates age of onset and progression of HD pathogenesis. Alfy is required in the adult brain for the autophagy-dependent clearance of proteinaceous deposits, and its depletion in mice and neurons derived from patient fibroblasts accelerates the aberrant accumulation of this pathological hallmark shared across adult-onset neurodegenerative diseases. These findings indicate that selectively compromising the ability to eliminate aggregated proteins is a pathogenic driver, and the selective elimination of aggregates may confer disease resistance.


Subject(s)
Adaptor Proteins, Signal Transducing/genetics , Autophagy-Related Proteins/genetics , Huntington Disease/genetics , Macroautophagy/genetics , Neurons/metabolism , Protein Aggregation, Pathological/genetics , Age of Onset , Animals , Cell Death/genetics , Disease Models, Animal , Female , Fibroblasts , Humans , Huntingtin Protein/genetics , Huntingtin Protein/metabolism , Huntington Disease/metabolism , Huntington Disease/physiopathology , Male , Mice , Mice, Knockout , Protein Aggregation, Pathological/metabolism , Protein Aggregation, Pathological/physiopathology
2.
Neurosci Lett ; 697: 29-33, 2019 04 01.
Article in English | MEDLINE | ID: mdl-29665429

ABSTRACT

Here we explore the neurodevelopmental aspects of macroautophagy (henceforth known as autophagy), the process by which cells remove and remodel their structure in a regulated and spatially restricted manner. Autophagy is a catabolic pathway in which cytosolic substances, such as protein complexes, lipids, and organelles, are engulfed by an autophagic vesicle. Degradation occurs once an autophagosome fuses with a lysosome, allowing the macromolecular cargo sequestered within the autophagic vesicle to be recycled. It is firmly established that autophagy plays a pivotal role in maintaining cellular homeostasis. Nevertheless, new evidence has emerged that the molecular mechanisms which regulate brain growth and neuronal connectivity involve autophagic processes. Our aim, as we endeavor to review data from model systems, is to show that autophagy performs a fundamental role in the development of the central nervous system (CNS). Moreover, we discuss human genetic data to underscore that mutations in autophagy-related genes are a contributing factor in childhood neurological disorders. To emphasize the importance of regulated vesicle transport pathways during the formation of the CNS, we discuss autophagy in relation to endosomal sorting to the lysosome, and explore how these mechanisms might intersect to regulate developmental events. We maintain that a deeper understanding of the function of autophagy in the CNS can shed new light on the biological basis of neurodevelopmental disorders.


Subject(s)
Nervous System Diseases/pathology , Neurodevelopmental Disorders/pathology , Animals , Autophagosomes/pathology , Autophagy/physiology , Brain/growth & development , Brain/pathology , Central Nervous System/growth & development , Central Nervous System/pathology , Child , Endosomes/pathology , Humans , Lysosomes/pathology , Neurons/pathology
3.
Elife ; 52016 09 20.
Article in English | MEDLINE | ID: mdl-27648578

ABSTRACT

The regulation of protein degradation is essential for maintaining the appropriate environment to coordinate complex cell signaling events and to promote cellular remodeling. The Autophagy linked FYVE protein (Alfy), previously identified as a molecular scaffold between the ubiquitinated cargo and the autophagic machinery, is highly expressed in the developing central nervous system, indicating that this pathway may have yet unexplored roles in neurodevelopment. To examine this possibility, we used mouse genetics to eliminate Alfy expression. We report that this evolutionarily conserved protein is required for the formation of axonal tracts throughout the brain and spinal cord, including the formation of the major forebrain commissures. Consistent with a phenotype reflecting a failure in axon guidance, the loss of Alfy in mice disrupts localization of glial guidepost cells, and attenuates axon outgrowth in response to Netrin-1. These findings further support the growing indication that macroautophagy plays a key role in the developing CNS.


Subject(s)
Brain/embryology , Neural Pathways/embryology , Neurons/physiology , Vesicular Transport Proteins/metabolism , Adaptor Proteins, Signal Transducing , Animals , Autophagy-Related Proteins , Gene Knockout Techniques , Mice, Inbred C57BL
4.
J Cell Sci ; 129(6): 1260-70, 2016 Mar 15.
Article in English | MEDLINE | ID: mdl-26818841

ABSTRACT

The accumulation of ubiquitylated proteinaceous inclusions represents a complex process, reflecting the disequilibrium between aggregate formation and aggregate clearance. Although decreasing aggregate formation or augmenting aggregate clearance will ultimately lead to a diminished aggregate burden, in terms of disease pathogenesis, the different approaches can have distinct outcomes. Using a novel cell-based assay that can distinguish newly formed versus preformed inclusions, we demonstrate that two proteins previously implicated in the autophagic clearance of expanded polyglutamine inclusions, HspB7 and Alfy (also known as WDFY3), actually affect very distinct cellular processes to affect aggregate burden. Using this cell-based assay, we also establish that constitutive expression of the aggregation-prone protein can measurably slow the elimination of protein aggregates, given that not all aggregates appear to be available for degradation. This new assay can therefore not only determine at what step a modifier might influence aggregate burden, but also can be used to provide new insights into how protein aggregates are targeted for degradation.


Subject(s)
Biochemistry/methods , HSP27 Heat-Shock Proteins/metabolism , Membrane Proteins/metabolism , Protein Aggregates , Transcription Factors/metabolism , Adaptor Proteins, Signal Transducing , Animals , Autophagy , Autophagy-Related Proteins , HSP27 Heat-Shock Proteins/genetics , HeLa Cells , Humans , Inclusion Bodies , Membrane Proteins/genetics , Mice , Proteolysis , Transcription Factors/genetics
5.
J Biol Rhythms ; 26(3): 200-9, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21628547

ABSTRACT

The neuropeptide vasoactive intestinal polypeptide (VIP) has emerged as a key candidate molecule mediating the synchronization of rhythms in clock gene expression within the suprachiasmatic nucleus (SCN). In addition, neurons expressing VIP are anatomically well positioned to mediate communication between the SCN and peripheral oscillators. In this study, we examined the temporal expression profile of 3 key circadian genes: Per1, Per2 , and Bmal1 in the SCN, the adrenal glands and the liver of mice deficient for the Vip gene (VIP KO), and their wild-type counterparts. We performed these measurements in mice held in a light/dark cycle as well as in constant darkness and found that rhythms in gene expression were greatly attenuated in the VIP-deficient SCN. In the periphery, the impact of the loss of VIP varied with the tissue and gene measured. In the adrenals, rhythms in Per1 were lost in VIP-deficient mice, while in the liver, the most dramatic impact was on the phase of the diurnal expression rhythms. Finally, we examined the effects of the loss of VIP on ex vivo explants of the same central and peripheral oscillators using the PER2::LUC reporter system. The VIP-deficient mice exhibited low amplitude rhythms in the SCN as well as altered phase relationships between the SCN and the peripheral oscillators. Together, these data suggest that VIP is critical for robust rhythms in clock gene expression in the SCN and some peripheral organs and that the absence of this peptide alters both the amplitude of circadian rhythms as well as the phase relationships between the rhythms in the SCN and periphery.


Subject(s)
ARNTL Transcription Factors/metabolism , Circadian Rhythm , Gene Expression Regulation , Period Circadian Proteins/metabolism , Suprachiasmatic Nucleus/metabolism , Vasoactive Intestinal Peptide/physiology , Adrenal Glands/metabolism , Animals , Genes, Reporter , In Situ Hybridization , Liver/metabolism , Luciferases/metabolism , Mice , Mice, Inbred C57BL , Mice, Knockout , Photoperiod
6.
Eur J Neurosci ; 31(5): 864-75, 2010 Mar.
Article in English | MEDLINE | ID: mdl-20180841

ABSTRACT

Previously, we have shown that mice deficient in either vasoactive intestinal peptide (VIP) or pituitary adenylate cyclase-activating polypeptide (PACAP) exhibit specific deficits in the behavioral response of their circadian system to light. In this study, we investigated how the photic regulation of the molecular clock within the suprachiasmatic nucleus (SCN) is altered by the loss of these closely-related peptides. During the subjective night, the magnitude of the light-induction of FOS and phosphorylated mitogen-activated protein kinase (p-MAPK) immunoreactive cells within the SCN was significantly reduced in both VIP- and PACAP-deficient mice when compared with wild-type mice. The photic induction of the clock gene Period1 (Per1) in the SCN was reduced in the VIP- but not in the PACAP-deficient mice. Baselines levels of FOS, p-MAPK or Per1 in the night were not altered by the loss of these peptides. In contrast, during the subjective day, light exposure increased the levels of FOS, p-MAPK and Per1 in the SCN of VIP-deficient mice, but not in the other genotypes. During this phase, baseline levels of these markers were reduced in the VIP-deficient mice compared with untreated controls. Finally, the loss of either neuropeptide reduced the magnitude of the light-evoked increase in Per1 levels in the adrenals in the subjective night without any change in baseline levels. In summary, our results indicate that both VIP and PACAP regulate the responsiveness of cells within the SCN to the effects of light. Furthermore, VIP, but not PACAP, is required for the appropriate temporal gating of light-induced gene expression within the SCN.


Subject(s)
Biological Clocks/physiology , Circadian Rhythm/physiology , Gene Expression Regulation/physiology , Pituitary Adenylate Cyclase-Activating Polypeptide/metabolism , Suprachiasmatic Nucleus/metabolism , Vasoactive Intestinal Peptide/metabolism , Animals , Gene Expression , Immunohistochemistry , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Mitogen-Activated Protein Kinases/biosynthesis , Mitogen-Activated Protein Kinases/genetics , Period Circadian Proteins/biosynthesis , Period Circadian Proteins/genetics , Proto-Oncogene Proteins c-fos/biosynthesis , Proto-Oncogene Proteins c-fos/genetics , Reverse Transcriptase Polymerase Chain Reaction
7.
J Neurophysiol ; 103(2): 632-40, 2010 Feb.
Article in English | MEDLINE | ID: mdl-19939959

ABSTRACT

In mammals, the precise circadian timing of many biological processes depends on the generation of oscillations in neural activity of pacemaker cells in the suprachiasmatic nucleus (SCN) of the hypothalamus. Understanding the ionic mechanisms underlying these rhythms is an important goal of research in chronobiology. Previous work has shown that SCN neurons express A-type potassium currents (IAs), but little is known about the properties of this current in the SCN. We sought to characterize some of these properties, including the identities of IA channel subunits found in the SCN and the circadian regulation of IA itself. In this study, we were able to detect significant hybridization for Shal-related family members 1 and 2 (Kv4.1 and 4.2) within the SCN. In addition, we used Western blot to show that the Kv4.1 and 4.2 proteins are expressed in SCN tissue. We further show that the magnitude of the IA current exhibits a diurnal rhythm that peaks during the day in the dorsal region of the mouse SCN. This rhythm seems to be driven by a subset of SCN neurons with a larger peak current and a longer decay constant. Importantly, this rhythm in neurons in the dorsal SCN continues in constant darkness, providing an important demonstration of the circadian regulation of an intrinsic voltage-gated current in mammalian cells. We conclude that the anatomical expression, biophysical properties, and pharmacological profiles measured are all consistent with the SCN IA current being generated by Kv4 channels. Additionally, these data suggest a role for IA in the regulation of spontaneous action potential firing during the transitions between day/night and in the integration of synaptic inputs to SCN neurons throughout the daily cycle.


Subject(s)
Circadian Rhythm/physiology , Ion Channel Gating/physiology , Membrane Potentials/physiology , Neurons/physiology , Potassium/metabolism , Shal Potassium Channels/physiology , Suprachiasmatic Nucleus/physiology , Animals , Feedback, Physiological/physiology , Male , Mice , Mice, Inbred C57BL
8.
ASN Neuro ; 1(3)2009 Jun 10.
Article in English | MEDLINE | ID: mdl-19570032

ABSTRACT

Genes responsible for generating circadian oscillations are expressed in a variety of brain regions not typically associated with circadian timing. The functions of this clock gene expression are largely unknown, and in the present study we sought to explore the role of the Per2 (Period 2) gene in hippocampal physiology and learned behaviour. We found that PER2 protein is highly expressed in hippocampal pyramidal cell layers and that the expression of both protein and mRNA varies with a circadian rhythm. The peaks of these rhythms occur in the late night or early morning and are almost 180° out-of-phase with the expression rhythms measured from the suprachiasmatic nucleus of the same animals. The rhythms in Per2 expression are autonomous as they are present in isolated hippocampal slices maintained in culture. Physiologically, Per2-mutant mice exhibit abnormal long-term potentiation. The underlying mechanism is suggested by the finding that levels of phosphorylated cAMP-response-element-binding protein, but not phosphorylated extracellular-signal-regulated kinase, are reduced in hippocampal tissue from mutant mice. Finally, Per2-mutant mice exhibit deficits in the recall of trace, but not cued, fear conditioning. Taken together, these results provide evidence that hippocampal cells contain an autonomous circadian clock. Furthermore, the clock gene Per2 may play a role in the regulation of long-term potentiation and in the recall of some forms of learned behaviour.

9.
BMC Neurosci ; 9: 63, 2008 Jul 10.
Article in English | MEDLINE | ID: mdl-18616823

ABSTRACT

BACKGROUND: The neuropeptide vasoactive intestinal peptide (VIP) is widely distributed in the adult central nervous system where this peptide functions to regulate synaptic transmission and neural excitability. The expression of VIP and its receptors in brain regions implicated in learning and memory functions, including the hippocampus, cortex, and amygdala, raise the possibility that this peptide may function to modulate learned behaviors. Among other actions, the loss of VIP has a profound effect on circadian timing and may specifically influence the temporal regulation of learning and memory functions. RESULTS: In the present study, we utilized transgenic VIP-deficient mice and the contextual fear conditioning paradigm to explore the impact of the loss of this peptide on a learned behavior. We found that VIP-deficient mice exhibited normal shock-evoked freezing behavior and increases in corticosterone. Similarly, these mutant mice exhibited no deficits in the acquisition or recall of the fear-conditioned behavior when tested 24-hours after training. The VIP-deficient mice exhibited a significant reduction in recall when tested 48-hours or longer after training. Surprisingly, we found that the VIP-deficient mice continued to express circadian rhythms in the recall of the training even in those individual mice whose wheel running wheel activity was arrhythmic. One mechanistic explanation is suggested by the finding that daily rhythms in the expression of the clock gene Period2 continue in the hippocampus of VIP-deficient mice. CONCLUSION: Together these data suggest that the neuropeptide VIP regulates the recall of at least one learned behavior but does not impact the circadian regulation of this behavior.


Subject(s)
Cell Cycle Proteins/physiology , Circadian Rhythm/physiology , Cognition/physiology , Nuclear Proteins/physiology , Transcription Factors/physiology , Vasoactive Intestinal Peptide/physiology , Animals , Behavior, Animal/physiology , Brain/metabolism , Brain/physiology , Cell Cycle Proteins/genetics , Corticosterone/blood , Hippocampus/metabolism , Hippocampus/physiology , In Situ Hybridization , Learning/physiology , Memory/physiology , Memory, Short-Term/physiology , Mice , Mice, Inbred C57BL , Mice, Knockout , Motor Activity/physiology , Nuclear Proteins/genetics , Period Circadian Proteins , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Vasoactive Intestinal Peptide, Type II/physiology , Suprachiasmatic Nucleus/metabolism , Suprachiasmatic Nucleus/physiology , Synaptic Transmission/physiology , Transcription Factors/genetics , Vasoactive Intestinal Peptide/deficiency , Vasoactive Intestinal Peptide/genetics
10.
J Comp Neurol ; 501(4): 526-42, 2007 Apr 01.
Article in English | MEDLINE | ID: mdl-17278130

ABSTRACT

Mutations in the gene encoding methyl CpG binding protein 2 (MeCP2) are the primary cause of the neurodevelopmental disorder Rett syndrome (RTT). Mecp2-deficient mice develop a neurological phenotype that recapitulates many of the symptoms of RTT, including postnatal onset of the neurological deficits. MeCP2 has two isoforms, MeCP2e1 and MeCP2e2, with distinct amino termini, which are generated by alternative splicing. We examined the distribution of the Mecp2 splice variants in the postnatal mouse brain by in situ hybridization and found regional and age-related differences in transcript abundance. In newborn mice, signals for total Mecp2 and the Mecp2e2 transcripts were widely distributed, with overlapping expression patterns throughout the brain. Expression of the Mecp2e2 splice variant became largely restricted to nuclei within the dorsal thalamus (DT) and cortical layer V in juvenile animals, a pattern that was maintained into adulthood. In contrast, the total Mecp2 riboprobe only weakly labeled the DT and cortical layer V in juvenile and adult animals, although it heavily labeled surrounding brain regions, suggesting that Mecp2e1 is the predominant transcript outside the thalamus. Quantitative real-time PCR was used to measure Mecp2e1 and Mecp2e2 abundance in the diencephalon of adult mice, demonstrating significantly more Mecp2e2 in the DT than in the hypothalamus, which is in agreement with the Mecp2e2 in situ hybridization. The differential distribution of the Mecp2e1 and Mecp2e2 transcripts indicates regional and developmental regulation of Mecp2 splicing in the postnatal mouse brain.


Subject(s)
Alternative Splicing , Brain/metabolism , Gene Expression Regulation, Developmental/physiology , Methyl-CpG-Binding Protein 2/genetics , Age Factors , Animals , Animals, Newborn , Brain/growth & development , Brain Mapping , Female , In Situ Hybridization/methods , Male , Mice , Mice, Inbred C57BL , RNA, Messenger , Reverse Transcriptase Polymerase Chain Reaction/methods
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