ABSTRACT
This paper presents data on a human allotypic polymorphism carried on a low density lipoprotein (As-LDL). The As polymorphism was investigated by a rabbit antiserum which was firstly used to analyze the serum level and the isoelectric point of the As lipoprotein in sera of different phenotype. This antiserum was also used to purify As-LDL by immunochromatography. Afterwards the peptidic maps of the APO proteins from different As-LDL were compared. While no correlation did result between the serum level and the serological phenotype of the As lipoprotein, the peptidic distribution of the APO parts from different As-LDL showed differences correlated to the allotypic phenotype.
Subject(s)
Lipoproteins, LDL/genetics , Animals , Apolipoproteins/analysis , Chromatography, Affinity , Humans , Immune Sera , Immunosorbent Techniques , Isoelectric Focusing , Lipoproteins, LDL/immunology , Lipoproteins, LDL/isolation & purification , Peptide Mapping , Phenotype , Polymorphism, Genetic , Rabbits , Thalassemia/blood , Thalassemia/geneticsABSTRACT
Alloimmunization of cattle revealed a polymorphism carried on a serum antigen displayed beta-electrophoretic mobility. A genetic test indicated that the antigen is inherited in a Mendelian manner, as product of a dominant allele at an autosomal locus. By immunochemical investigation the allotypic determinant was found to be located on IgA: there were in fact no differences between the peptidic map of the molecule carrying the allotypic determinant and that of IgA from positive sera. Moreover the peptidic map of IgA from sera lacking of the allotype resulted lacking of an anodic peptide, thus suggesting that the polymorphism is indeed due to such a peptide.
Subject(s)
Cattle/blood , Immunoglobulin A/analysis , Immunoglobulin Allotypes/analysis , Animals , Blood Proteins/genetics , Blood Proteins/immunology , Female , Immunodiffusion , Immunoelectrophoresis , Isoantibodies/immunology , Polymorphism, GeneticABSTRACT
This paper presents data on the identification of an allotypic polymorphism carried on a beta-lipoprotein in human serum. The antigen, whose inheritance is investigated in 78 families, is coded by a polymorphic locus (As) existing in 4 allelic states. The products of these alleles exhibit a serological linear subtyping pattern with respect to the alloantisera recognizing the allotypes. The allelic frequencies prove different in healthy (beta beta) and thalassemic (beta beta thal) sample populations, thus suggesting linkage dysequilibrium between the recessive alleles of the As and beta loci.