ABSTRACT
A combination of microscopy (video-enhanced contrast microscopy) and spectrophotometry (which analyses online the spectra from the microscopic picture) is presented. This set-up allows the in vivo microscopic observation of cellular and subcellular structures as well as simultaneous online determination of cellular chromophores. To test our equipment we measured spectra of isolated red blood cells and NG 108-15 cells and correlated these spectra with the corresponding video-enhanced microscopic picture. By labelling specific cell organelles of astrocytes with in vivo fluorescent dyes, we visualized mitochondria and captured the corresponding spectra.
Subject(s)
Astrocytes/ultrastructure , Hybrid Cells/ultrastructure , Microscopy, Video/methods , Mitochondria/ultrastructure , Spectrophotometry/methods , Animals , Erythrocytes/ultrastructure , Fluorescent Dyes , Glioma , Mice , Neuroblastoma , Rats , Rhodamine 123 , RhodaminesABSTRACT
Cephalometric analysis is essential in order to evaluate treatment results. 202 patients were investigated for the validity of the norm values according to Hasund. In order to test the validity of the growth prediction methods of Björk and Petrovic 23 class II div. 1 and 21 class III patients were analysed before and after treatment and the treatment results achieved compared with the growth prediction values. The results of the norm value tests showed that the population in Saxonia is more retrognath than that in the northern region of Germany (Hasund norm group). The study also showed that the Björk growth prediction and the Petrovic growth categories were not specific enough and it is therefore suggested that separate growth prediction methods should be used in class II div. 1 and class III groups.
Subject(s)
Cephalometry , Maxillofacial Development , Adolescent , Child , Child, Preschool , Evaluation Studies as Topic , Female , Humans , Male , Malocclusion, Angle Class II/physiopathology , Malocclusion, Angle Class II/therapy , Malocclusion, Angle Class III/physiopathology , Malocclusion, Angle Class III/therapy , Prognosis , Reference Values , Treatment OutcomeABSTRACT
The utilization of a computer supported cephalometric system using the analysis introduced by Hasund is demonstrated. In comparison to the traditional measurement by hand the advantages of a computerized analysis are: Time reduction, minimization of evaluation errors, the use of individualized norm values and the advantages in recording.
Subject(s)
Cephalometry , Malocclusion/diagnostic imaging , Orthodontics, Corrective , Radiographic Image Interpretation, Computer-Assisted , Humans , Orthodontics, Corrective/instrumentation , TelemetryABSTRACT
Radiographic cephalometric measurements are very important for the orthodontic diagnosis. For the computerized analysis 10 percent of time is necessary comparable with the volume of time for the analysis by hand. For the computerized analysis were used the following hardware: AC 7100, Digitizer K 6405, Printer K 6314. After input of 19 skeletal points with the digitizer the results will printed as a hardcopy. The cephalometric method was adapted by the method after Hasund. The software was written in Turbo-Pascal. The computerized cephalometric analysis is a part of our computerized orthodontic analysis programme.
