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1.
Phys Chem Chem Phys ; 20(19): 13419-13429, 2018 May 16.
Article in English | MEDLINE | ID: mdl-29722404

ABSTRACT

Ultrasmall silicon carbide nanoparticles (SiC USNPs) are very promising biomarkers for developing new applications in diagnostics, cell monitoring or drug delivery, even though their interaction with biological molecules such as different proteins has not yet been investigated in detail. In this study, the biological behaviour of SiC USNPs in a medium modeling a living organism was investigated in detail through the dependence of the fluorescence on interactions between bovine serum albumin (BSA) and SiC USNPs. The interaction shows transient nanoparticle-protein associations due to the restricted diffusion behaviour of the nanoparticles in the vicinity of a protein. The transient association manifests in a complex fluorescence quenching mechanism where the dynamic component was dominated by Förster resonance energy transfer. By studying SiC nanoparticles of different sizes, it can be concluded that the transient effect is an ultrasmall nanoparticle behaviour.


Subject(s)
Carbon Compounds, Inorganic/chemistry , Fluorescent Dyes/chemistry , Nanoparticles/chemistry , Serum Albumin, Bovine/chemistry , Silicon Compounds/chemistry , Animals , Binding Sites , Biomarkers/chemistry , Cattle , Fluorescence Resonance Energy Transfer , Kinetics , Particle Size , Protein Binding , Spectrometry, Fluorescence , Spectroscopy, Fourier Transform Infrared/methods , Surface Properties , Thermodynamics
2.
Food Chem ; 213: 799-805, 2016 Dec 15.
Article in English | MEDLINE | ID: mdl-27451250

ABSTRACT

HR-CS-GFAAS methods were developed for the fast determination of Cu in domestic and commercially available Hungarian distilled alcoholic beverages (called pálinka), in order to decide if their Cu content exceeds the permissible limit, as legislated by the WHO. Some microliters of samples were directly dispensed into the atomizer. Graphite furnace heating programs, effects/amounts of the Pd modifier, alternative wavelengths (e.g., Cu I 249.2146nm), external calibration and internal standardization methods were studied. Applying a fast graphite furnace heating program without any chemical modifier, the Cu content of a sample could be quantitated within 1.5min. The detection limit of the method is 0.03mg/L. Calibration curves are linear up to 10-15mg/L Cu. Spike-recoveries ranged from 89% to 119% with an average of 100.9±8.5%. Internal calibration could be applied with the assistance of Cr, Fe, and/or Rh standards. The accuracy of the GFAAS results was verified by TXRF analyses.


Subject(s)
Alcoholic Beverages/analysis , Copper/analysis , Graphite , Spectrophotometry, Atomic , Calibration , Food Analysis , Limit of Detection
3.
Talanta ; 147: 271-5, 2016 Jan 15.
Article in English | MEDLINE | ID: mdl-26592606

ABSTRACT

The determination of Al contaminant and the main component Si in silicon carbide (SiC) nanocrystals with the size-distribution of 1-8nm dispersed in an aqueous solution was developed using high-resolution continuum source graphite furnace atomic absorption spectrometry (HR-CS-GFAAS). The vaporization/atomization processes were investigated in a transversally heated graphite atomizer by evaporating solution samples of Al and Si preserved in various media (HCl, HNO3). For Si, the best results were obtained by applying a mixture of 5µg Pd plus 5µg Mg, whereas for Al, 10µg Mg (each as nitrate solution) was dispensed with the samples, but the results obtained without modifier were found to be better. This way a maximum pyrolysis temperature of 1200°C for Si and 1300°C for Al could be used, and the optimum (compromise) atomization temperature was 2400°C for both analytes. The Si and Al contents of different sized SiC nanocrystals, dispersed in aqueous solutions, were determined against aqueous (external) calibration standards. The correlation coefficients (R values) of the calibrations were found to be 0.9963 for Si and 0.9991 for Al. The upper limit of the linear calibration range was 2mg/l Si and 0.25mg/l Al. The limit of detection was 3µg/l for Si and 0.5µg/l for Al. The characteristic mass (m0) was calculated to be 389pg Si and 6.4pg Al. The Si and Al content in the solution samples were found to be in the range of 1.0-1.7mg/l and 0.1-0.25mg/l, respectively.


Subject(s)
Aluminum/analysis , Carbon Compounds, Inorganic/chemistry , Graphite/chemistry , Nanoparticles/chemistry , Silicon Compounds/chemistry , Silicon/analysis , Spectrophotometry, Atomic/methods , Aluminum/chemistry , Hot Temperature , Limit of Detection , Silicon/chemistry
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