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Bioresour Technol ; 176: 47-55, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25460983

ABSTRACT

This feasibility study investigated a two-step biorefining approach to increase the value gained by recycling of organic municipal solid waste. Firstly, lactic acid was produced via batch fermentation at 37°C using the indigenous microbiome. Experiments revealed an optimal fermentation period of 24h resulting in high yields of lactic acid (up to 37gkg(-1)). The lactic acid proportion of total volatile fatty acid content reached up to 83%. Lactobacilli were selectively enriched to up to 75% of the bacterial community. Additionally conversion of organic matter to lactic acid was increased from 22% to 30% through counteracting end product inhibition by continuous lactic acid extraction. Secondly, fermentation residues were used as co-substrate in biomethane production yielding up to 618±41Nmlbiomethaneg(-1) volatile solids. Digestate, the only end product of this process can be used as organic fertilizer.


Subject(s)
Biofuels , Biotechnology/methods , Lactic Acid/biosynthesis , Lactobacillus/metabolism , Methane/biosynthesis , Refuse Disposal/methods , Base Sequence , Cluster Analysis , DNA Primers/genetics , Denaturing Gradient Gel Electrophoresis , Fermentation , High-Throughput Nucleotide Sequencing , Lactic Acid/isolation & purification , Lactobacillus/genetics , Lactobacillus/growth & development , Molecular Sequence Data , Phylogeny , Time Factors
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