[Application of autologous and allogenic keratynocyte cultures in burn management]. / Zastosowanie autologicznych i allogenicznych keratynocytów pochodzacych z hodowli w leczeniu oparzen.

First attempts to culture keratinocytes with intention of treating burn wounds started in the 19th century (Reverdin). Fast development of tissue engineering was connected to cell culture technology improvement in the fifties. Tissue culture of isolated keratinocytes was introduced by J.G. Rheinwald and H. Green. This method was modificated many times but is still used. Autologous keratinocytes culture was applied to burn management in 1981. Primary cell cultures became more popular in burn treatment. "Cell factories" and bioreactors are used to enhance proliferation to obtain huge amounts of cells in a short time. Cells are also seeded on matrixes made from glicosamonogycans, collagen, elastin or fibrin. These products are used as a skin substitutes.

[Changes in arylsulphatase activity in blood serum in patients with breast cancer before and after treatment]. / Zmiany aktywnosci arylosulfatazy w surowicy u chorych z rakiem sutka przed i po leczeniu.

We evaluated arylsulphatase activity in 52 women with breast cancer. In women with breast cancer this enzyme activity is 2,5-fold higher than in healthy women. After operation and chemiotherapy arylsulphatase activity decreased to normal values. There is correlation between enzyme activity and tumor size and number of metastases. In group with bigger tumor and higher number of metastases arylsulphatase activity is markedly higher.

Influence of autologous serum on rabbit chondrocytes proliferation in vitro. Comparing study to standard sera supplements.

Background. Autologous chondrocyte transplantation (ACT) is a method in reconstructive surgery. Fetal bovine serum (FBS) is still a "gold standard" for media supplementation in many laboratories. Prion and virus diseases are danger for patients treated witch ACT if FBS is used. The aim of this study was to establish the utility of media for chondrocytes culture supplemented with Autologous Serum (AS) or serum substitute (Ultroser G. UG) instead of FBS.
Material and methods. Primary rabbit articular chondrocytes culture was established and cultures in MHCH (Chondrocytes Culture Medium) medium with 10% FBS during first 3 passages. The influence of different sera in various concentrations of 10%, 5% and 2,5%, while Ultroser G in 2%, 1% and 0,5%. Ceels were transferred in amount of 104 into the 24-well plates containing tested media. After 96 hours cells were counted using trypan blue test and photo documentation was done. Means were compared using Student-T test.
Results. AS in concentration of 10% stimulated cell growth to 14,93+/-2,03 x 104 cell/well. Medium supplemented with the same concentration of FBS allowed to obtain 6,56+/-1,08 x 104 cell/well. Transfer from medium supplemented with 10% of FBS to flask containing Ultroser G stopped proliferation rate. Cell viability in AS and FBS supplemented media was over 90% while 80,8-8809% in UG.
Conclusion. 10% FBS and AS conditioned media are both suitable for chondrocytes culture. AS seems to be the serum conditioner for chondrocytes propagation.