ABSTRACT
The present study describes the analysis of bacterial communities on historical window glass by denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA fragments. So far, only a few studies have been published in which the microflora and the corrosion mechanisms of glass surfaces have been investigated. Some microorganisms, especially fungi, have been isolated from different glass samples in the past. However, our results demonstrate that bacterial communities on biodeteriorated glass surfaces are much more complex than previously believed. In addition, bacteria were identified, which have never been isolated from glass samples before.
Subject(s)
Bacteria/isolation & purification , Electrophoresis, Agar Gel/methods , Glass , Polymerase Chain Reaction/methods , RNA, Ribosomal, 16S/genetics , Bacteria/genetics , DNA, Bacterial/analysis , DNA, Bacterial/genetics , DNA, Ribosomal/analysis , DNA, Ribosomal/genetics , Ecosystem , Gene Amplification , Genes, rRNA , Molecular Sequence DataABSTRACT
9-Methoxytariacuripyrone, a nitro-aromatic compound isolated from Aristolochia brevipes showed strong mutagenic activity in strain TA98, TA100 and some YG strains of Salmonella typhimurium with and without S9 mix. Incubation with cytosol resulted in a heavy increase in mutagenicity. When incubated with microsomes the activity was dramatically decreased. The results are discussed in view of the enzymes possibly involved in activation and detoxification of the compound. The role of the basic structure on the mutagenicity mediated through the nitro group was also considered.
