Index models to evaluate the risk of phosphorus and nitrogen loss at catchment scales.

This paper investigates index models as a tool to estimate the risk of N and P source strengths and loss at the catchment scale. The index models assist managers in improving the focus of remediation actions that reduce nutrient delivery to waterbodies. N and P source risk factors (e.g. soil nutrient concentrations) and transport risk factors (e.g. distance-to-streams) are used to determine the overall risk of nutrient loss for a case study in the Tuross River catchment of coastal southeast Australia. In the development of the N index model for Tuross, particulate N was considered important based on the observed event water quality data. In contrast to previous N index models, erosion and contributing distance were therefore included in the Tuross River catchment N index. Event-based water quality monitoring, and soil information, or in data-poor catchments conceptual understanding, are essential to represent catchment-scale processes. The techniques have high applicability in other catchments, and are complementary to other modelling techniques such as process-based semi-distributed modelling. Index models generally provide much more detailed spatial resolution than fully- or semi-distributed conceptual modelling approaches. Semi-distributed models can be used to quantify nutrient loads and provide overall direction to set the broad focus of management. Index models can then be used to refine on-the-ground investigations and investment priorities. In this way semi-distributed models can be combined with index models to provide a set of powerful tools to influence management decisions and outcomes.

Suspended sediment, nitrogen and phosphorus concentrations and exports during storm-events to the Tuross estuary, Australia.

This paper presents a process for estimating pollutant loads from water quality data, to improve catchment-scale modelling in the region for resource management purposes. It describes a program to estimate suspended sediment, total and dissolved nitrogen and phosphorus loads to the Tuross estuary from the Tuross River catchment (1810 km(2)) of coastal southeast Australia. Event-based water quality sampling results obtained during storm events in 2005 are presented. Event 1, during July 2005 was the largest storm event in terms of peak flow for 3.5 years. Other events monitored were also in July, November and December 2005. The early July 2005 event had a flow-weighted mean suspended sediment (SS) concentration during the first 4 days of 63 mg L(-1). Of the events monitored, this was unusual as it was preceded by drought and had the largest SS concentrations (peaking at 180 mg L(-1)) during the rising-stage. In contrast, the November event had a much lower flow-weighted SS mean (28 mg L(-1)), even though peak flow magnitudes were similar. The July and November 2005 events had peak flows of 12,360 and 11,330 ML day(-1). Low-cost rising-stage siphon samplers were used to collect samples during the rapidly rising phase of these events. The use of such samplers and consideration of time-lead/lag flow adjustments, quantified using cross-correlation analysis to account for hysteresis effects, were incorporated into the load estimation techniques. The technique is a potentially useful approach for understanding relationships between water quality concentrations and flow for modelling catchment source strengths and transport processes.

Effect of high arterial carbon dioxide tension on efficiency of immunoglobulin G absorption in calves.

OBJECTIVES: To determine whether high PaCO2 reduced apparent efficiency of IgG absorption (AEA) in calves and whether assisted ventilation of calves with high PaCO2 increased AEA. ANIMALS: 48 Holstein calves. PROCEDURES: Arterial and venous blood samples were collected 1, 13, and 25 hours after birth; an additional venous sample was collected at 37 hours after birth. Arterial samples were analyzed for PaCO2, PaO2, pH, and bicarbonate and base excess concentrations; venous samples were analyzed for plasma IgG concentrations. On the basis of 1-hour PaCO2, calves were assigned to nonrespiratory acidosis (PaCO2 < 50 mm Hg; n = 19) or respiratory acidosis (PaCO2 > or = 50 mm Hg; 29) groups. Calves in the respiratory acidosis group were assigned randomly to receive no further treatment (n = 17) or to be given 5 minutes of assisted ventilation (12). All calves received between 1.8 and 2 L of colostrum 2, 14, 26, and 38 hours after birth. Plasma volume and AEA were determined 25 hours after birth. RESULTS: 1-hour PaCO2 had no effect on AEA or on plasma IgG concentrations determined 13, 25, or 37 hours after birth. Artificial ventilation had no effect on plasma IgG concentration or AEA. CONCLUSIONS AND CLINICAL RELEVANCE: Lack of effect of 1-hour PaCO2 on AEA and IgG concentration indicated that calves compensated for moderate acid-base imbalances associated with birth. Calves born with high PaCO2 achieved adequate plasma IgG concentrations if fed an adequate amount of high-quality colostrum early in life. The effect of artificial ventilation on PaCO2 was temporary and did not increase AEA.

Nutrient and immunity transfer from cow to calf pre- and postcalving.

Nutritional and management strategies for dairy cattle are designed to prepare the cow for lactation and to minimize the incidence of metabolic diseases around calving. However, strategies initiated during the dry period should also consider the potential effects on the calf prior to and after calving. Fetal requirements for energy and protein are significant, particularly during the last trimester of gestation. Energy requirements increase to 1.3 to 1.5 times maintenance in late pregnancy; therefore, the formulation of rations for dry cows must contain sufficient energy to support fetal growth plus maintenance. Protein requirements during pregnancy increase, particularly during the last 2 mo. Colostrum is a source of immune components and nutrients to the neonate and contains more protein, immunoglobulins (Ig), nonprotein nitrogen, fat, ash, vitamins, and minerals than does milk. Because some vitamins do not cross the placental barrier, colostrum is the primary source of these nutrients for the calf after birth. Colostrum from cows that are not supplemented with vitamin E during the dry period may provide inadequate vitamin E to calves after birth. The Ig concentration in colostrum is not markedly affected by prepartum protein nutrition; diets containing high crude protein (CP) generally increase the nonprotein fraction of colostrum, but low CP diets do not affect the CP or Ig concentration of colostrum. However, data from beef calves suggest that absorption of IgG may be impaired when low protein diets are fed during the dry period. Diets for dry cows may be balanced to reduce the cation to anion ratio, which may reduce the incidence of parturient paresis. Recent research also suggests that these diets might increase the incidence of calves born in respiratory acidosis, which may impair the acquisition of passive immunity.

Effects of a colostrum replacement product derived from serum on immunoglobulin G absorption by calves.

Calves are born hypogammaglobulinemic and rely on immunoglobulin (Ig) from colostrum to obtain passive immunity. Previous research has indicated that colostrum supplements derived from milk are less effective than is maternal colostrum in providing adequate IgG to neonatal calves. Our objective was to determine the absorption of IgG by newborn calves fed a USDA food-grade colostrum supplement derived from bovine serum or fed pooled maternal colostrum. Holstein calves (n = 20; 10 bulls) were removed from the dam within 1 h of birth and were housed in individual stalls for the 24-h study. Calves were fed 2 L of colostrum or colostrum replacer at 1.5 and 13.5 h (+/- 0.1 h). Calves were blocked by colostrum pool, and replacer was fed to provide equal intakes of IgG within blocks. Jugular blood was collected at 1 and 24 h (+/- 0.1 h) for analysis of IgG by radial immunodiffusion. At 24 h, calves were injected with 1.5 ml of Evans blue dye to estimate plasma volume. Mean plasma IgG at 24 h of age was 7.3 +/- 0.4 g/L and was affected by an interaction of block and treatment. Apparent efficiency of IgG absorption of 24 h was reduced when 750 g of the colostrum replacement product were fed but was increased when 266 g of colostrum replacement product were fed. Mean plasma volume was unaffected by treatment and was 3.5 +/- 0.2 L or 9.1% of BW. These data indicate that efficiency of IgG absorption from the colostrum replacement product may be affected by amount of material fed. Proteins other than IgG in the colostrum replacement product might have reduced the efficiency of IgG absorption.

Estimation of plasma volume in Holstein and Jersey calves.

The concentration of immunoglobulin (Ig) G in the blood of neonatal calves shortly after birth is a widely used criterion to determine the degree of acquisition of passive immunity. Another method used to determine the biological mechanisms of IgG absorption is calculation of the apparent efficiency of IgG absorption. Estimation of the efficiency of IgG absorption requires the estimation of plasma volume in neonatal calves. Previous estimates of plasma volume in a few calves of varying breeds have been made; the estimates ranged from 7 to 14.5% of body weight (BW). Holstein (n = 97 from four farms) and Jersey (n = 49 from one farm) calves were fed fresh maternal colostrum or colostrum that had been previously frozen. Calves were fed 2 L of colostrum at 4.1 h (SE = 0.2; range = 0.3 to 11.0 h) and 12 h later. Plasma volume was measured by determining the concentration of Evans' blue dye in a jugular blood sample collected 10 min after injection of approximately 1.5 ml of 1.5% Evans' blue dye. Factors that affected plasma volume (milliliters) were BW, breed, and age at sampling; r2 of the regression was 0.60. Factors that affected plasma volume (percentage of BW) were BW, breed, and age at sampling; r2 of the regression was 0.08. Mean plasma volume for all calves was 3162 ml (SE = 79) and was 9.86% of birth BW (SE = 0.15%). Mean plasma volume was 2250 ml (9.71% of BW) and 3623 ml (9.94% of BW) for Jersey and Holstein calves, respectively. Body weight was the best predictor of plasma volume.

Effects of lasalocid in milk replacer of calf starter on health and performance of calves challenged with Eimeria species.

Holstein bull calves (n = 48) were purchased from local sale barns at 3 to 7 d of age and were assigned randomly to a 2 x 2 factorial arrangement of lasalocid in milk replacer (0 or 80 mg/kg) and in calf starter (3 or 44 mg/kg of dry matter). On d 10 after arrival, calves were orally dosed with 100,000 Eimeria oocysts. Intakes of calf starter and milk replacer, body weight (BW), BW gain, excretion of fecal oocysts, and fecal scores were determined. Calves fed lasalocid in milk replacer consumed more calf starter, had greater BW gain, shed fewer oocysts in feces, and scoured less frequently and less severely than did calves fed no lasalocid or those fed lasalocid in calf starter alone. The combination of lasalocid in milk replacer and in calf starter did not improve performance above that of calves fed lasalocid in milk replacer alone. Low intake of calf starter prior to weaning may provide an insufficient amount of lasalocid to control effectively the effects of coccidiosis when calves are infected with Eimeria at an early age. Use of coccidiostats in milk replacers may reduce the effects of coccidiosis in young calves that are infected with Eimeria at an early age.

Body weight gain, feed efficiency, and fecal scores of dairy calves in response to galactosyl-lactose or antibiotics in milk replacers.

Holstein bull calves (n = 96) were purchased at approximately 7 d of age and were assigned to receive 400, 450, 500, and 550 g/d of milk replacer solids during wk 1 to 4, respectively. Treatments were nonmedicated milk replacer plus dried whey, medicated milk replacer (138 mg/kg of oxytetracycline and 276 mg/kg of neomycin) plus dried whey, or nonmedicated milk replacer plus whey processed with beta-galactosidase to contain 15% galactosyl-lactose. Processed whey was added to provide 1% of dry matter as galactosyl-lactose; an equivalent amount of dried whey was added to the other treatments to provide 6.3% of dry matter daily. Intake of milk replacer and fecal scores were measured daily. No calf starter or hay was fed. Body weights were measured weekly from arrival to 26 d. Serum immunoglobulin G, measured 1 d after arrival, averaged 18.3 g/L. Intake of milk replacer plus additive during the 26-d study was 445 g/d and did not vary with treatment. Body weight and body weight gain were increased by 52 and 72 g/d in response to antibiotics and galactosyl-lactose, respectively. Severity of scours and number of days scouring tended to be reduced when calves were fed milk replacer containing galactosyl-lactose or antibiotics.

Effects of high pressure on the channel gated by the quisqualate-sensitive glutamate receptor of locust muscle and its blockade by ketamine; a single-channel analysis.

The effects of high pressure on the channel gating kinetics of the quisqualate-sensitive L-glutamate receptor (qGluR) of locust muscle have been investigated using a megaohm seal patch-clamp technique. Pressure was applied with helium gas and recordings were carried out at 20.5 degrees C with Rb+ as the main charge-carrying cation in the patch pipette. The mean open time of the qGluR channel was unaffected by 10 and 30 MPa, but it was significantly reduced at 50 MPa. A high proportion of brief openings (mean 0.808 ms) was seen at 50 MPa but not at lesser pressures. Also, in contrast to lesser pressures, 50 MPa prolonged the mean closed time and reduced both the frequency and probability of channel opening. 10(-6) M ketamine significantly reduced the mean channel open time, as previously reported. A pressure of 10 MPa which alone had no effect on the qGluR channel, restored the mean open time in the presence of 10(-6) M ketamine to the value obtained in the absence of the anaesthetic. This implies the shortening of qGluR channel open time by ketamine involves a large + delta V and, therefore, probably conformational changes in the channel. However 10 MPa did not restore the distribution of open times to normal.

Podophyllum toxicity: a report of a fatal case and a review of the literature.

A 59-yr-old male ingested 10 g of podophyllum in a fatal suicide attempt. Previously unreported nuclear and cytoplasmic changes were observed in circulating leukocytes. Symptoms did not occur until 10 h after ingestion with loss of reflexes, coma, and a marked lactic acidosis. Despite hemoperfusion, the patient expired 39 h after ingestion. The literature on podophyllum toxicity is reviewed.

Intrauterine contraceptive device failures in renal transplant patients.

Concern is voiced regarding sexually active renal transplant patients and their use of the IUD for contraception. Two patients of this type conceived while receiving immunosuppressive therapy. The possible mechanisms of the IUD in preventing conception are mentioned. Immunosuppression may significantly alter these actions, thus allowing fertilization and implantation to occur. Alternate means of contraception are suggested in place of IUDs for these patients.

PIP: 2 case studies are presented where patients with functioning renal transplants on immunosuppressive treatment conceived while using a copper IUD for contraception. Since ovulation recurs from between 1 and 12 months following return to normal renal function after renal transplant, the sexually active woman must utilize some form of contraception. For those women who opt for contraception of a nonpermanent nature, there are several problems. Oral contraceptives often cause a reversible elevation in blood pressure in these patients and they may also have some detrimental effects on the sodium and water balance. The diaphragm, foam, and condom may be used but without high motivation from the couple, unplanned and unwanted pregnancies often result. IUD use can open a renal patient to the risk of infection or, as in these case reports, to contraceptive failure due to a decrease in effectiveness in association with immunosuppressive therapy. In animal studies it was shown that the antifertility effect of the IUD was associated with the administration of Cytoxan and Leukeran (immunosuppressive agents) along with a decrease in IgG and IgM. Although IUD mechanism of action is not totally understood, it does seem that an intact immune system is required for effectiveness. Thus renal transplant patients should be informed about the use of other agents in order to avoid unwanted pregnancy.