ABSTRACT
An artificial respirator was used to ensure successful and precise artificial respiration in short and long term experiments during radical exposure of the caudal cervical region and thoracic cavity to investigate cytological changes in the carotid body of mature chickens (Gallus domesticus). Modification of the respirator to stabilize gas flow, temperature and humidity consisted of replacing the ball check valve in the heating and humidifying cylinder with a fine regulating stopcock in the water outlet of the cylinder and inserting eight glass rods in a circle inside the cylinder. Accumulation of water condensate in the trachea and lungs during long term experiments was prevented by inserting a cotton filter moisture trap in the tracheal cannula.
Subject(s)
Carotid Body/surgery , Chickens/surgery , Ventilators, Mechanical , AnimalsABSTRACT
Sterba's technique to detect neurosecretory substances was applied to carotid body tissue, surrounding branchial derivatives, ganglion nodosum and the carotid arterial wall. Mainly a yellowish-green, a yellowish-red and a bright yellow fluorescence was observed as well as greenish-yellow fibres with varicosities and terminal knobs. The metachromatic reaction of the technique was briefly discussed.
Subject(s)
Carotid Body/analysis , Animals , Carotid Body/cytology , Chickens/anatomy & histology , Chickens/metabolism , Histocytochemistry , Male , Microscopy, Fluorescence , Ultimobranchial Body/analysisABSTRACT
10 clinically healthy white leghorn males were utilised for this investigation. Apart from toluidine blue-pyronin-stained Araldite sections, uranyl acetate and lead monoxide staining revealed three types of epithelioid cells. Nuclear and cytoplasmic contents, especially three types of granules, conveniently designated as q, r and p granules, are described in detail and tabulated. Technical difficulties in obtaining carotid body tissue as well as clear-stained sections for ultastructural studies are mentioned.
Subject(s)
Carotid Body/ultrastructure , Chickens/anatomy & histology , Animals , Cell Nucleus/ultrastructure , Cytoplasm/ultrastructure , Cytoplasmic Granules/ultrastructure , Epithelium/ultrastructure , Male , Microscopy, Electron , Organoids/ultrastructureABSTRACT
The histotopography of the silvery-white glistening carotid body and the branchial derivates in the cranial thoracic inlets as well as the histocytology of the particular organ were revealed by various microtechniques. Three types of randomly distributed epithelioid cells, many capillaries, and small and large sinuses are observable. Myelinated fibres are sparsely distributed. 25 clinically healthy white leghorn males were used for this investigation.
Subject(s)
Carotid Body/cytology , Chickens/anatomy & histology , Animals , Arterioles/cytology , Carotid Arteries/cytology , Connective Tissue Cells , Cytological Techniques , Epithelial Cells , Fibroblasts/cytology , Male , Muscle, Smooth/cytology , Nerve Fibers/cytology , Nerve Fibers, Myelinated/cytologyABSTRACT
The anatotopography of cranial thoracic inlets, the carotid body and associated structures in the caudal pole of the thyroid gland are described. The source from which the various groups of fowls (n = 65) were obtained as well as the varied localisation of the carotid body are indicated. In this investigation, the carotid body was found to be situated mostly (49 cases) either on or in the immediate vicinity of blood vessels (carotid and inferior thyroid artery) and only in 14 cases in close association with the parathyroids.
