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2.
Leukemia ; 31(3): 645-653, 2017 03.
Article in English | MEDLINE | ID: mdl-27677741

ABSTRACT

While clinical benefit of the proteasome inhibitor (PI) bortezomib (BTZ) for multiple myeloma (MM) patients remains unchallenged, dose-limiting toxicities and drug resistance limit the long-term utility. The E3 ubiquitin ligase Skp1-Cullin-1-Skp2 (SCFSkp2) promotes proteasomal degradation of the cell cycle inhibitor p27 to enhance tumor growth. Increased SKP2 expression and reduced p27 levels are frequent in human cancers and are associated with therapeutic resistance. SCFSkp2 activity is increased by the Cullin-1-binding protein Commd1 and the Skp2-binding protein Cks1B. Here we observed higher CUL1, COMMD1 and SKP2 mRNA levels in CD138+ cells isolated from BTZ-resistant MM patients. Higher CUL1, COMMD1, SKP2 and CKS1B mRNA levels in patient CD138+ cells correlated with decreased progression-free and overall survival. Genetic knockdown of CUL1, COMMD1 or SKP2 disrupted the SCFSkp2 complex, stabilized p27 and increased the number of annexin-V-positive cells after BTZ treatment. Chemical library screens identified a novel compound, designated DT204, that reduced Skp2 binding to Cullin-1 and Commd1, and synergistically enhanced BTZ-induced apoptosis. DT204 co-treatment with BTZ overcame drug resistance and reduced the in vivo growth of myeloma tumors in murine models with survival benefit. Taken together, the results provide proof of concept for rationally designed drug combinations that incorporate SCFSkp2 inhibitors to treat BTZ resistant disease.


Subject(s)
Antineoplastic Agents/pharmacology , Drug Resistance, Neoplasm/genetics , Multiple Myeloma/genetics , Multiple Myeloma/metabolism , Pharmacogenetics , S-Phase Kinase-Associated Proteins/metabolism , Small Molecule Libraries , Adaptor Proteins, Signal Transducing/genetics , Animals , Cell Line, Tumor , Cell Survival/drug effects , Cullin Proteins/genetics , Disease Models, Animal , Drug Discovery , Drug Synergism , Female , Gene Expression , Gene Knockdown Techniques , Humans , Mice , Multiple Myeloma/drug therapy , Multiple Myeloma/mortality , Pharmacogenetics/methods , Prognosis , Proteasome Inhibitors/pharmacology , S-Phase Kinase-Associated Proteins/antagonists & inhibitors , S-Phase Kinase-Associated Proteins/genetics , Xenograft Model Antitumor Assays
4.
Leukemia ; 29(11): 2184-91, 2015 Nov.
Article in English | MEDLINE | ID: mdl-26108695

ABSTRACT

Although the therapeutic benefit of proteasome inhibition in multiple myeloma remains unchallenged, drug resistance inevitably emerges through mechanisms that remain elusive. Bortezomib provokes unwanted protein accumulation and the endoplasmic reticulum stress to activate the unfolded protein response (UPR) and autophagy as compensatory mechanisms that restore protein homeostasis. High-throughput screens to detect pharmacologics that modulated autophagy to enhance the anti-myeloma effect of bortezomib revealed metformin, a widely used antidiabetic agent with proven efficacy and limited adverse effects. Metformin co-treatment with bortezomib suppressed induction of the critical UPR effector glucose-regulated protein 78 (GRP78) to impair autophagosome formation and enhance apoptosis. Gene expression profiling of newly diagnosed myeloma patient tumors further correlated the hyperexpression of GRP78-encoding HSPA5 with reduced clinical response to bortezomib. The effect of bortezomib was enhanced with metformin co-treatment using myeloma patient tumor cells and the chemoresistant, stem cell-like side population that may contribute to disease recurrence. The relevance of the findings was confirmed in vivo as shown by metformin co-treatment with bortezomib that delayed the growth of myeloma xenotransplants. Taken together, our results suggest that metformin suppresses GRP78, a key driver of bortezomib-induced autophagy, and support the pharmacologic repositioning of metformin to enhance the anti-myeloma benefit of bortezomib.


Subject(s)
Antineoplastic Agents/pharmacology , Autophagy/drug effects , Bortezomib/pharmacology , Heat-Shock Proteins/antagonists & inhibitors , Metformin/pharmacology , Multiple Myeloma/drug therapy , Animals , Apoptosis/drug effects , Cell Line, Tumor , Drug Synergism , Endoplasmic Reticulum Chaperone BiP , Female , Heat-Shock Proteins/physiology , Humans , Mice , Phosphatidylinositol 3-Kinases/physiology
5.
Leukemia ; 29(3): 727-38, 2015 Mar.
Article in English | MEDLINE | ID: mdl-25234165

ABSTRACT

Evading apoptosis is a cancer hallmark that remains a serious obstacle in current treatment approaches. Although proteasome inhibitors (PIs) have transformed management of multiple myeloma (MM), drug resistance emerges through induction of the aggresome+autophagy pathway as a compensatory protein clearance mechanism. Genome-wide profiling identified microRNAs (miRs) differentially expressed in bortezomib-resistant myeloma cells compared with drug-naive cells. The effect of individual miRs on proteasomal degradation of short-lived fluorescent reporter proteins was then determined in live cells. MiR-29b was significantly reduced in bortezomib-resistant cells as well as in cells resistant to second-generation PIs carfilzomib and ixazomib. Luciferase reporter assays demonstrated that miR-29b targeted PSME4 that encodes the proteasome activator PA200. Synthetically engineered miR-29b replacements impaired the growth of myeloma cells, patient tumor cells and xenotransplants. MiR-29b replacements also decreased PA200 association with proteasomes, reduced the proteasome's peptidase activity and inhibited ornithine decarboxylase turnover, a proteasome substrate degraded through ubiquitin-independent mechanisms. Immunofluorescence studies revealed that miR-29b replacements enhanced the bortezomib-induced accumulation of ubiquitinated proteins but did not reveal aggresome or autophagosome formation. Taken together, our study identifies miR-29b replacements as the first-in-class miR-based PIs that also disrupt the autophagy pathway and highlight their potential to synergistically enhance the antimyeloma effect of bortezomib.


Subject(s)
Antineoplastic Agents/pharmacology , Boronic Acids/pharmacology , Gene Expression Regulation, Neoplastic , MicroRNAs/genetics , Multiple Myeloma/drug therapy , Multiple Myeloma/genetics , Pyrazines/pharmacology , RNA, Small Interfering/genetics , Animals , Apoptosis/drug effects , Bortezomib , Cell Line, Tumor , Drug Resistance, Neoplasm/drug effects , Drug Resistance, Neoplasm/genetics , Genetic Vectors , Humans , Lentivirus/genetics , Mice , Mice, Inbred NOD , MicroRNAs/metabolism , Multiple Myeloma/mortality , Multiple Myeloma/pathology , Neoplasm Transplantation , Nuclear Proteins/genetics , Nuclear Proteins/metabolism , Ornithine Decarboxylase/genetics , Ornithine Decarboxylase/metabolism , Phagosomes/drug effects , Phagosomes/metabolism , Primary Cell Culture , Proteasome Endopeptidase Complex/drug effects , Proteasome Endopeptidase Complex/metabolism , RNA, Small Interfering/metabolism , Signal Transduction , Survival Analysis
6.
Leukemia ; 28(4): 732-8, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24714346

ABSTRACT

Theragnostics represent cutting-edge, multi-disciplinary strategies that combine diagnostics with therapeutics in order to generate personalized therapies that improve patient outcome. In oncology, the approach is aimed at more accurate diagnosis of cancer, optimization of patient selection to identify those most likely to benefit from a specific therapy and to generate effective therapeutics that enhance patient survival. MicroRNAs (miRNAs) are master regulators of the human genome that orchestrate myriad cellular pathways to control growth during physiologic and pathologic conditions. Compelling evidence shows that miRNA deregulation promotes events linked to tumor initiation, metastasis and drug resistance as seen in multiple myeloma (MM), an invariably fatal hematologic malignancy. miRNAs are readily detected in body fluids, for example, serum, plasma, urine, as well as circulating tumor cells to demonstrate their potential as readily accessible, non-invasive diagnostic and prognostic biomarkers and potential therapeutics. Specific miRNAs are aberrantly expressed early in myelomagenesis and may more readily detect high-risk disease than current methods. Although only recently discovered miRNAs have rapidly advanced from preclinical studies to evaluation in human clinical trials. The development of miRNA theragnostics should provide widely applicable tools for the targeted delivery of personalized medicines to improve the outcome of patients with MM.


Subject(s)
MicroRNAs/analysis , Multiple Myeloma/genetics , Gene Expression Regulation, Neoplastic , Humans , MicroRNAs/physiology , Multiple Myeloma/diagnosis , Multiple Myeloma/pathology , Multiple Myeloma/therapy , Neoplastic Cells, Circulating , Precision Medicine , Prognosis , Transcriptome
7.
Prog Clin Biol Res ; 274: 251-67, 1988.
Article in English | MEDLINE | ID: mdl-2841673

ABSTRACT

The reduction of O2 to H2O2 requires two protons as well as two electrons. Thus, activation of dioxygen reasonably may involve either general or specific acid catalysis. Consequently, the reduction of O2 to H2O2 could exhibit a kinetic solvent isotope effect (KSIE). The reaction catalyzed by the mononuclear Cu(II) enzyme, galactose oxidase does exhibit a KSIE (+1.55). The pL-rate profile exhibits an alkaline shift in D2O which can be attributed to the differential partitioning of H+ versus D+ between bulk water and a metal-bound H2O (delta pKa = +0.19). A variety of spectral evidence places an equatorial, Cu(II)-liganded water molecule at the active site of galactose oxidase. The analysis of the KSIE data is detailed and the potential generality of the function of such metal-bound H2O at other type 2 Cu(II) sites is discussed.


Subject(s)
Galactose Oxidase/metabolism , Kinetics , Oxidation-Reduction , Oxygen Consumption , Protein Binding , Protons , Solvents
8.
Biochemistry ; 26(12): 3429-36, 1987 Jun 16.
Article in English | MEDLINE | ID: mdl-2820469

ABSTRACT

Solvent and solvent proton dependent steps involved in the mechanism of the enzyme galactose oxidase have been examined. The deuterium kinetic solvent isotope effect (KSIE) on the velocity of the galactose oxidase catalyzed oxidation of methyl beta-galactopyranoside by O2 was measured. Examination of the thermodynamic activation parameters for the reaction indicated that the isotope effect was attributable to a slightly less favorable delta H value, consistent with a KSIE on proton transfer. A detailed kinetic analysis was performed, examining the effect of D2O on the rate of reaction over the pH range 4.8-8.0. Both pL-rate profiles exhibited bell-shaped curves. Substitution of D2O as solvent shifted the pKes values for the enzymic central complex: pKes1 from 6.30 to 6.80 and pKes2 from 7.16 to 7.35. Analysis of the observed shifts in dissociation constants was performed with regard to potential hydrogenic sites. pKes1 can be attributed to a histidine imidazole, while pKes2 is tentatively assigned to a Cu2+-bound water molecule. A proton inventory was performed (KSIE = +1.55); the plot of kcat vs. mole fraction D2O was linear, indicating the existence of a single solvent-derived proton involved in a galactose oxidase rate-determining step (or steps). The pH dependence of CN- inhibition was also examined. The Ki-pH profile indicated that a group ionization, with pKa = 7.17, modulated CN- inhibition; Ki was at a minimum when this group was in the protonated state. The inhibition profile followed the alkaline limit of the pH-rate profile for the enzymic reaction, suggesting that the group displaced by CN- was also deprotonating above pH 7.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Galactose Oxidase/metabolism , Cyanides/pharmacology , Deuterium , Deuterium Oxide , Kinetics , Mitosporic Fungi/enzymology , Protons , Solvents , Water
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