ABSTRACT
In order to monitor the antibody response to feline immunodeficiency virus (FIV) in cats, following experimental and natural infection, enzyme-linked immunosorbent assays (ELISAs) were developed using recombinant env and gag proteins and p24-specific monoclonal antibodies. It was shown that in experimentally infected cats an env protein-specific antibody response was directly followed by a gag protein-specific response. Furthermore, an ELISA for the detection of env protein-specific serum antibodies proved more sensitive in identifying experimentally and naturally infected cats than ELISAs demonstrating gag protein-specific antibodies. It was concluded that, like in HIV infection of humans, the detection of env protein-specific serum antibodies in addition to gag protein-specific antibodies is not only an important tool in the diagnosis of the infection but also in studies concerning the pathogenesis of the disease.
Subject(s)
Antibodies, Viral/blood , Feline Acquired Immunodeficiency Syndrome/immunology , Gene Products, env/immunology , Gene Products, gag/immunology , Immunodeficiency Virus, Feline/immunology , Animals , Antibodies, Monoclonal/immunology , Antibody Specificity , Blotting, Western , Cats , Enzyme-Linked Immunosorbent Assay , Female , Recombinant Proteins/immunology , Specific Pathogen-Free OrganismsABSTRACT
The immunoreactivity of a panel of monoclonal antibodies raised to tomato spotted wilt virus (TSWV) was examined in enzyme-linked immunosorbent assays (ELISA) and dot immunobinding assays (DIBA) procedures. MAbs 6.12.15 and 2.9 were specific for the nucleocapsid protein of TSWV. The sensitivity of the two immunoassays was compared with that of a dot-blot hybridization technique using riboprobes (RNA transcripts) to TSWV M RNA. Using deproteinized plant extracts or purified virus preparations, as little as 1 pg RNA could be detected. Although an ELISA using MAb 6.12.15, a DIBA procedure using MAb 3.22.6 and the dot-blot hybridization, detected several TSWV isolates in different host species equally well, the ELISA was most precise and most suitable for routine diagnosis in the field.
Subject(s)
Antibodies, Monoclonal , Plant Viruses/immunology , RNA Probes , Animals , Antibodies, Monoclonal/isolation & purification , Antibody Specificity , Antigens, Viral/analysis , Enzyme-Linked Immunosorbent Assay/methods , Evaluation Studies as Topic , Immunoblotting/methods , Mice , Mice, Inbred BALB C , Nucleic Acid Hybridization , RNA/geneticsABSTRACT
Murine lymphocyte hybridomas which produce neutralizing or non-neutralizing monoclonal antibodies to different type 1, 2 or 3 poliovirus strains were isolated. The majority of these monoclonal antibodies reacted with antigenic determinants present on different poliovirus strains of the same type. However, hybridomas producing monoclonal antibodies specific for each of the three Sabin vaccine strains were also generated.
Subject(s)
Antibodies, Monoclonal/immunology , Antibodies, Viral/immunology , Poliovirus Vaccine, Oral/immunology , Poliovirus/immunology , Animals , Antigens, Viral , Enzyme-Linked Immunosorbent Assay , Epitopes , Hybridomas , Mice , Neutralization Tests , Poliovirus/classificationABSTRACT
After a brief review of the present knowledge about canine viral enteritis, the role played by parvoviral, coronaviral and rotaviral infections in contagious diarrhoea in dogs in the Netherlands is discussed. For this purpose a serologic survey, pathologic findings in dogs, and the demonstration of parvoviral antigen with an immunofluorescence test and with a newly developed haemadsorption-elution-haemagglutination assay (HEHA) are presented. It is concluded that infections with canine parvovirus, coronavirus and rotavirus appear widespread among dog populations in the Netherlands.
Subject(s)
Enteritis/veterinary , Reoviridae Infections/veterinary , Animals , Antigens, Viral/isolation & purification , Coronaviridae/immunology , Coronaviridae Infections/veterinary , Disease Outbreaks/veterinary , Dogs , Enteritis/etiology , Enteritis/microbiology , Netherlands , Parvoviridae/immunology , Reoviridae Infections/microbiology , Rotavirus/immunologyABSTRACT
Summary After a brief review of the present knowledge about canine viral enteritis, the role played by parvoviral, coronaviral and rotaviral infections in contagious diarrhoea in dogs in the Netherlands is discussed. For this purpose a serologic survey, pathologic findings in dogs, and the demonstration of parvoviral antigen with an immunofluorescence test and with a newly developed haemadsorption-elution-haemagglutination assay (HEHA) are presented. It is concluded that infections with canine parvovirus, coronavirus and rotavirus appear widespread among dog populations in the Netherlands.
