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An easy, quick and cheap high-throughput method for yeast DNA extraction from microwell plates.

Drumonde-Neves, J; Vieira, E; Lima, M T; Araujo, I; Casal, M; Schuller, D.

J Microbiol Methods ; 93(3): 206-8, 2013 Jun.

Article in English | MEDLINE | ID: mdl-23545448

ABSTRACT

We herein present a high-throughput and cheap method for yeast DNA isolation in a 96 well microplate. About 1500 yeast isolates can be processed within one working day and final DNA concentrations are suitable for direct application in PCR-based molecular typing methods.

Subject(s)

DNA, Fungal/isolation & purification , Microbiological Techniques/methods , Molecular Biology/methods , Specimen Handling/methods , Yeasts/genetics , Microbiological Techniques/economics , Molecular Biology/economics , Molecular Typing/methods , Mycological Typing Techniques/methods , Specimen Handling/economics , Time Factors

ABSTRACT

Subject(s)

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