An eye-tracking study on visual perception of vegetation permeability in virtual reality forest exposure.

Previous studies have confirmed the significant effects of single forest stand attributes, such as forest type (FT), understory vegetation cover (UVC), and understory vegetation height (UVH) on visitors' visual perception. However, rarely study has yet clearly determined the relationship between vegetation permeability and visual perception, while the former is formed by the interaction of multiple forest stand attributes (i.e., FT, UVC, UVH). Based on a mixed factor matrix of FT (i.e., coniferous forests and broadleaf), UVC level (i.e., 10, 60, and 100%), and UVH level (0.1, 1, and 3 m), the study creates 18 immersive virtual forest videos with different stand attributes. Virtual reality eye-tracking technology and questionnaires are used to collect visual perception data from viewing virtual forest videos. The study finds that vegetation permeability which is formed by the interaction effect of canopy density (i.e., FT) and understory density (i.e., UVC, UVH), significantly affects participant's visual perception: in terms of visual physiology characteristics, pupil size is significantly negatively correlated with vegetation permeability when participants are viewing virtual reality forest; in terms of visual psychological characteristics, the understory density formed by the interaction of UVC and UVH has a significant impact on visual attractiveness and perceived safety and the impact in which understory density is significantly negatively correlated with perceived safety. Apart from these, the study finds a significant negative correlation between average pupil diameter and perceived safety when participants are viewing virtual reality forests. The findings may be beneficial for the maintenance and management of forest parks, as well as provide insights into similar studies to explore urban public green spaces.

Conformational Change and Activity Enhancement of Rabbit Muscle Lactate Dehydrogenase Induced by Polyethyleneimine.

For a better understanding on the interaction between polyethyleneimine (PEI) and proteins, spectroscopic studies including UV-vis absorption, resonance Rayleigh scattering, fluorescence, and circular dichroism were conducted to reveal the conformational change of rabbit muscle lactate dehydrogenase (rmLDH) and related to the bioactivity of the enzyme. Regardless of the electrostatic repulsion, PEI could bind on the surface of rmLDH, a basic protein, via hydrogen binding of the dense amine groups and hydrophobic interaction of methyl groups. The competitive binding by PEI led to a reduction of the binding efficiency of rmLDH toward ß-nicotinamide adenine dinucleotide, the coenzyme, and sodium pyruvate, the substrate. However, the complex formation with PEI induced a less ordered conformation and an enhanced surface hydrophobicity of rmLDH, facilitating the turnover of the enzyme and generally resulting in an increased activity. PEI of higher molecular weight was more efficient to induce alteration in the conformation and catalytic activity of the enzyme.

Rapid and sensitive detection of interleukin-6 in serum via time-resolved lateral flow immunoassay.

Interleukin 6 (IL-6) is an interleukin that acts as both a proinflammatory and anti-inflammatory cytokine. It can be used as a potential diagnostic biomarker for sepsis. The aim of this study was to establish an easy-to-use detection kit for rapid, quantitative and on-site detection of IL-6. To develop the new IL-6 quantitative detecting kit, a double-antibody sandwich immunofluorescent assay was employed based on europium nanoparticles (Eu-np) combined with lateral flow immunoassay (LFIA). The performance of the new developed kit was evaluated in the aspects of parallel analysis, linearity, sensitivity, precision, accuracy, specificity and clinical sample analysis. Two-hundred and fourteen serum samples were used to carry out the clinical sample analysis. The new IL-6 quantitative detecting kit exhibited a wide linear range (2-500 pg/mL) and a good sensitivity (0.37 pg/mL). The intra-assay coefficient of variation (CV) and the inter-assay CV were 5.92%-8.87% and 7.59%-9.04%, respectively. The recovery rates ranged from 102% to 106%. Furthermore, a high correlation (n = 214, r = 0.9756, p < 0.01) was obtained when compared with SIEMENS CLIA IL-6 kit. Thus, the new quantitative method for detecting IL-6 has been successfully established. The results indicated that the newly-developed strip based on Eu-np combined with LFIA was a facile, fast, highly sensitive, low-cost, reliable biosensor and suitable for rapid and point-of-care test (POCT) for IL-6 in serum.

Suitability of Taxodium distichum for Afforesting the Littoral Zone of the Three Gorges Reservoir.

The littoral zone ecosystem of the Three Gorges Reservoir (TGR) has become significantly degraded by annual cycles of prolonged winter flooding and summer drought. For purposes of flood control and sediment management, the water level in the reservoir is lowered by 30 m during the summer monsoon season and raised again to 175 m above sea level each year at the end of the monsoon period. To explore an effective way to promote biodiversity and associated ecosystem services, we examined Taxodium distichum as a species for afforesting the littoral zone. Sapling growth variations were measured after two rounds of winter flooding. Dominant influence factors were determined by redundancy analysis. Herb community similarities between the experimental afforested areas and nearby control areas were assessed to detect the ecosystem influence of the experimental afforestation. 94.5% of saplings planted at elevations above 168 m survived. All measured growth indices (tree height, diameter at breast height, crown width and foliage density) decreased as the flood depth increased. Completely submerged saplings had a mean dieback height of -0.65 m. Greater initial foliage density led to increased tree height and stem diameter. Shannon-Wiener indices were not significantly different between plots in experimental and control areas, but the low similarity of herb communities between experimental and control areas (0.242 on average) suggested that afforestation would enrich plant community structure and improve littoral zone ecosystem stability. Because littoral zone afforestation provides several ecosystem services (habitat, carbon sink, water purification and landscaping), it is a promising revegetation model for the TGR.