ABSTRACT
Ybox binding protein1 (YB1) has been identified as an oncoprotein in various malignancies. The aim of this study was to investigate the biological role of YB1 and its association with epithelialtomesenchymal transition (EMT) in colorectal cancer (CRC). The expression of YB1 and three EMTrelated proteins (Ecadherin, Ncadherin and vimentin) was analyzed in 80 CRC and matched normal tissue samples, by immunohistochemistry. The results indicated that the expression of YB1 was higher in CRC tissue samples than that in matched normal controls and was significantly correlated with tumor differentiation, tumor invasion, lymph node metastasis and distant metastases. Furthermore, analysis showed that YB1 expression was negatively correlated with Ecadherin and positively correlated with Ncadherin and vimentin expression. In vitro assays showed that knockdown of YB1 inhibited the proliferation, apoptosis resistance, invasion and migration of the HT29 CRC cell line. Of note, following knockdown of YB1, Ecadherin expression was elevated whereas Ncadherin and vimentin expression was reduced. Taken together, these results suggest that YB1 promotes the malignant progression of CRC in part through the induction of EMT, and YB1 may therefore be a potential novel target for CRC treatment.
Subject(s)
Adenocarcinoma/metabolism , Colorectal Neoplasms/metabolism , Y-Box-Binding Protein 1/genetics , Adenocarcinoma/secondary , Apoptosis , Cell Movement , Cell Proliferation , Colorectal Neoplasms/pathology , Epithelial-Mesenchymal Transition , Female , Gene Knockdown Techniques , HT29 Cells , Humans , Lymphatic Metastasis , Male , Middle Aged , Neoplasm Invasiveness , Y-Box-Binding Protein 1/biosynthesisABSTRACT
OBJECTIVE: To compare laparoscopic Nissen fundoplication (LNF)and Toupet laparoscopic fundoplication (LTF) with respect to treatment outcomes and postoperative complications. METHODS: PubMed, Medline, Embase and the Cochrane Library were searched. Only randomized controlled trials (RCTs) comparing laparoscopic Nissen and Toupet fundoplication were included. Outcomes evaluation included occurrences of heartburn, reflux, difficulty swallowing, chest pain, abdominal distention, failure to hiccup, diarrhea, and early complications and degree of patient satisfaction at early (three to six months) and later (one to three years) post-operative periods. RESULTS: Of 939 patients in seven RCTs, 478 received LNF and 461 received LTF. For both groups, control of reflux was good and occurrence of heartburn was similar (P>0.05). A lower incidence of postoperative dysphagia for both early and later post-operative periods, but a higher overall complication rate in early post-operative period were observed in the LTF group (P<0.05). Patient satisfaction was similar (P>0.05). CONCLUSIONS: LNF and LTF are both safe and effective. The adoption of procedure should be based on the patient status and surgeon experience.