ABSTRACT
OBJECTIVE: To study the structure-activity relationships of phenylethanoid glycosides in plants of Cistanche salsa on antioxidative activity. METHODS: By the assay systems of DPPH*, the antioxidant activity of six phenylethanoid glycosides from plants of Cistanche salsa was determined to investigate the relationship between the antioxidant activities and phenylethanoid glycosides's structural characteristics. RESULTS: The antioxidative activity of phenylethanoid glycosides was variant with dose-dependent effect. The sequence of the strength of the antioxidative activity of the six components was shown to be 2'-Acetylacteoside > Acteoside > or = Tubuloside B > or = Isoacteoside > Echinacoside > Cistanoside A. CONCLUSION: The antioxidative activity of phenylethanoid glycosides is related to the number of phenolic hydroxyl, steric hindrance, 2-acetyl on the middle glucopyranose, and the location of phenolic hydroxyl. Additionally, it may be related to the alpha, beta-unsaturated ketone of phenl-2-propenoyl.
Subject(s)
Antioxidants/chemistry , Antioxidants/pharmacology , Cistanche/chemistry , Glycosides/chemistry , Plants, Medicinal/chemistry , Antioxidants/isolation & purification , Free Radical Scavengers , Glucosides/chemistry , Glucosides/pharmacology , Glycosides/pharmacology , Hydroxyl Radical/metabolism , Molecular Structure , Structure-Activity RelationshipABSTRACT
OBJECTIVE: To study the chemical constituents of cultivated Cistanche salsa. METHODS: Compounds were isolated and purified on several chromatography, and then were identified by physico-chemical properties and structurally elucidated by spectral analysis. RESULTS: Seven compounds were isolated and identified as beta-sitosterol (I), daucosterol (II), beta-sitosteryl glucoside 3'-O-heptadecoicate (III), 8-hydroxygeraniol 1-beta-D-glucopyranoside (IV), 2-methanol-5-hydroxy-pyridine (V), betaine (VI), galactitol (VII). CONCLUSION: The chemical constituents of artificial cultivated Cistanche salsa are studied for the first time. Among them, compound III and IV are isolated from the plant for the first time, compound V is isolated from this genus for the first time.
Subject(s)
Cistanche/chemistry , Glucosides/isolation & purification , Plants, Medicinal/chemistry , Pyridoxine/analogs & derivatives , Betaine/chemistry , Betaine/isolation & purification , Cistanche/growth & development , Glucosides/chemistry , Plants, Medicinal/growth & development , Pyridoxine/chemistry , Pyridoxine/isolation & purification , Sitosterols/chemistry , Sitosterols/isolation & purificationABSTRACT
OBJECTIVE: To study the chemical constituents of Hedysarum polybotrys. METHOD: Chromatographic methods were used to isolate compounds from H. polybotrys and chemical and spectral methods were used to elucidate the structures of the isolated compounds. RESULT: Five compounds, N,N,N-trimethyl-tropaphone inner salt (hypaphorine) (1), octadecyl-3-methoxy-4-hydroxy-benzeneacrylate (2), 5,7,4'-trihydroxy-dihydroflavanone 5,7-di-O-beta-D-glucopyranoside (3), 3,4,5-trimethoxy cinnamic acid methy ester (4) and vanillic acid (5), were isolated from the roots of H. polybotrys. CONCLUSION: Compounds 1, 2 and 3 were obtained from this plant for the first time, while compounds 1 and 2 were isolated from Hedysarum for the first time.
Subject(s)
Acrylates/isolation & purification , Fabaceae/chemistry , Indoles/isolation & purification , Plants, Medicinal/chemistry , Acrylates/chemistry , Indoles/chemistry , Molecular Conformation , Molecular Structure , Plant Roots/chemistryABSTRACT
AIM: To study the chemical constituents of Hedysarum polybotrys Hand.-Mazz.. METHODS: The compounds were separated with Sephadex LH-20 and silica gel column chromatography, and their structures were identified on the basis of spectral analysis. RESULTS: Five compounds were identified as hedysalignan A (1), isoliquiritigenin (2), formononetin (3), calycosin (4) and ononin (5). CONCLUSION: Hedysalignan (1) is a new compound and the others were obtained from the plant for the first time.
Subject(s)
Chalcone/analogs & derivatives , Drugs, Chinese Herbal/isolation & purification , Fabaceae/chemistry , Furans/isolation & purification , Glucosides/isolation & purification , Chalcone/chemistry , Chalcone/isolation & purification , Chalcones , Drugs, Chinese Herbal/chemistry , Furans/chemistry , Glucosides/chemistry , Isoflavones/chemistry , Isoflavones/isolation & purification , Molecular Conformation , Molecular Structure , Plant Roots/chemistry , Plants, Medicinal/chemistryABSTRACT
OBJECTIVE: To investigate the chemical constituents from the root and rhizome of Rheum rhizastachyum. METHOD: The compounds were isolated by column chromatography on silica gel, Sephadex LH-20, MCI-gel CHP-20P separately and their structures were elucidated by chemical and spectral technology. RESULT: Six compounds were isolated and identified as chrysophanol, emodin, gallic acid, sucrose, 1-O-beta-D-glucopyranosyl-chrysophanols, 1-O-beta-D-glucopyranosyl-emodin. CONCLUSION: All the compounds were obtained from this plant for the first time.
Subject(s)
Anthraquinones/isolation & purification , Emodin/isolation & purification , Plants, Medicinal/chemistry , Rheum/chemistry , Anthraquinones/chemistry , Emodin/chemistry , Gallic Acid/chemistry , Gallic Acid/isolation & purification , Plant Roots/chemistry , Rhizome/chemistryABSTRACT
OBJECTIVE: To summarize the pharmacological function of oleanolic acid. METHOD: Based on the documents and related achievements, the pharmacological function and its mechanism including its clinical use were summed up. RESULT AND CONCLUSION: Oleanolic Acid is a natural chemical which has wide-ranging function and abundant source. But we should place the emphasis on improving its biotic utilization and effect on clinical treatment.
Subject(s)
Hepatitis, Viral, Human/drug therapy , Oleanolic Acid/pharmacology , Phytotherapy , Plants, Medicinal/chemistry , Adjuvants, Immunologic/pharmacology , Animals , Antineoplastic Agents, Phytogenic/pharmacology , Antiviral Agents/pharmacology , Humans , Hypoglycemic Agents/pharmacology , Oleanolic Acid/isolation & purificationABSTRACT
OBJECTIVE: To study the chemical constituents in the root of Hedysarum polybotrys Hand.-Mazz.. METHOD: The constituents were isolated by Sephadex LH-20 and silica gel column chromatography, and their structures were identified on the basis of spectral analysis. RESULT: Five compounds, medicarpin (I), 3-hydroxy-9-methoxycoumestan (II), 3, 9-dihydroxycoumestan (III), beta-sitosterol (IV), beta-sitosterol-3-O-beta-D-glucoside (V) were obtained. CONCLUSION: Compounds II, III, V were obtained from the plant for the first time.
