Transcriptome-metabolome analysis of fatty acid of Bamei pork and Gansu Black pork in China.

To study the difference in transcriptome level of fatty acid metabolism pathway in Bamei pork and the difference of pork quality caused by the difference. In this study, Bamei pigs breeding in Huzhu farm of QingHai province were selected as the test object, compared with Gansu Black pigs. Four indexes of nutmeg acid (DX1), palmitic acid (DX2), stearic acid (DX3) and linoleic acid (DX4) were set. The expression profiles of fat metabolism related genes between the two groups samples were analysed by GCMS metabolomics and transcriptomics, then coexpression network analysis were conducted to obtain phenotypic related genes. The results showed that the metabolic levels of DX3 and DX4 were significantly higher than those of other fatty acids. Among these differences, the ENSSSCG00000024681 (G1) and ENSSSCG00000036883 (G2) genes play important regulatory roles in fatty acid metabolism, and the upregulated expression of their gene obviously affects the level of fatty acid metabolism, thereby affecting the quality and taste of pork. In addition, we found that there was a good correlation between the same lines, and the genetic traits of the hybrid lines of Bamei pig and Black pig are more inclined to Bamei pig. In the independent fatty acid metabolism, "Mg2+"and flavin adenine dinucleotide are more active, which plays an important role in energy utilization. Therefore, we can be inferred that the metabolism of stearic acid and linoleic acid are important fatty acids for pork quality. It also further confirms that the research method of combined omics is of great significance for the study of species traits and gene functions.

Soil physical properties response to tillage practices during summer fallow of dryland winter wheat field on the Loess Plateau.

Soil physical properties are a greatly important part of the soil and indicator of soil quality, which can directly affect soil nutrient turnover and crop yields in dryland. This study was carried out with three tillage practices during the summer fallow season since 2011, including no tillage (NT), plow tillage (PT), and subsoiling (ST) in dryland winter wheat fields of the Loess Plateau. Results showed that soil tillage during the summer fallow had a small effect on soil bulk density (ρ b) in the 0-50-cm soil profile before sowing and after harvesting of winter wheat. Soil ρ b under NT at a depth of 20-30 cm was significantly greater than those under PT in both seasons. Both soil gravimetric water content (θ g) and volumetric moisture content (θ v) after harvesting increased by 28.8-78.6% and 37.5-87.3%, respectively, compared with those before sowing. Adoption of PT significantly increased soil θ g and θ v in the entire 0-50-cm profile before sowing compared with NT and ST (P < 0.05). In addition, there was a small effect on soil porosity (e.g., total porosity, air-filled porosity, and capillary porosity) in the profile of 0-50 cm both before sowing and after harvesting. Overall, short-term tillage during summer fallow mainly affected soil water content in the 0-50-cm soil profile, and it had a slight effect on other physical soil properties.