Body dysmorphic disorder symptoms in patients with melasma.

BACKGROUND: Body dysmorphic disorder (BDD) is a psychiatric condition characterized by extreme preoccupation with non-existent or minor defects in appearance, disrupting daily functioning. Melasma is a common concern among BDD patients with dermatological conditions. This study aimed to estimate the incidence and characteristics of BDD in patients with melasma, and compare the psychological condition of patients and age- and sex-matched healthy controls. METHODS: Patients with melasma and healthy controls were screened using the BDD Questionnaire (BDDQ), Self-rating Anxiety Scale (SAS), and Self-rating Depression Scale (SDS). A questionnaire was administered to collect demographic information and clinical characteristics. Standard statistical tests were conducted, such as descriptive analysis, chi-square, and nonparametric tests. The level of statistical significance for all tests was set at a P < 0.05. RESULTS: Among the 470 patients with melasma included in the study, 53 were positive for BDDQ in the preliminary screening and were associated with a history of treatment, higher Melasma Area and Severity Index (MASI), and Melasma Quality of Life (MELASQoL) scores, and higher SAS and SDS scores. The positive rate of BDDQ ranged from 0.4%, using the most stringent criteria to assess melasma severity, to 11.3%, without using objective criteria. Compared to healthy controls, patients with melasma had a higher positive rate of BDDQ and higher SAS and SDS scores. CONCLUSION: In patients with melasma who exaggerate the severity of the disease, psychiatric treatment and the patient-physician relationship have a positive effect. This study assessed the positive rate of BDDQ in melasma and proposed the feasibility of psychiatric treatment for patients with melasma.

Manipulating Redox Kinetics using p-n Heterojunction Biservice Matrix as both Cathode Sulfur Immobilizer and Anode Lithium Stabilizer for Practical Lithium-Sulfur Batteries.

As an attractive high-energy-density technology, the practical application of lithium-sulfur (Li-S) batteries is severely limited by the notorious dissolution and shuttle effect of lithium polysulfides (LiPS), resulting in sluggish reaction kinetics and uncontrollable dendritic Li growth. Herein, a p-n typed heterostructure consisting of n-type MoS2 nanoflowers embedded with p-type NiO nanoparticles is designed on carbon nanofibers (denoted as NiO-MoS2 @CNFs) as both cathode sulfur immobilizer and anode Li stabilizer for practical Li-S batteries. Such p-n typed heterostructure is proposed to establish the built-in electric field across the heterointerface for facilitated the positive charge to reach the surface of NiO-MoS2 , meanwhile inherits the excellent LiPS adsorption ability of p-type NiO nanoparticles and catalytic ability of n-type MoS2 . As the anode matrix, the implementation of NiO-MoS2 heterostructure can prevent the growth of Li dendrites by enhancing the lithiophilicity and reducing local current density. The obtained Li-S full battery exhibits an ultra-high areal capacity over 7.3 mAh cm-2 , far exceeding that of current commercial Li-ion batteries. Meanwhile, a stable cycling performance can be achieved under low electrolyte/sulfur ratio of 5.8 µL mg-1 and negative/positive capacity ratio of 1. The corresponding pouch cell maintains high energy density of 305 Wh kg-1 and stable cycling performance under various bending angles.

Effect of acupuncture in eczema: An overview of systematic reviews.

BACKGROUND: Eczema is a common chronic relapsing inflammatory skin disease, which is characterized by intense itching. Acupuncture can be effective for eczema, and it is thus regarded as a common complementary treatment. OBJECTIVE: The intention of this overview is to methodically appraise and synthesize evidence about systematic reviews/meta-analyses (SRs/MAs) on acupuncture in eczema. METHODS: We searched for SRs/MAs of acupuncture with eczema in eight databases. We evaluated the methodological quality by Assessing the Methodological Quality of Systematic Reviews 2 (AMSTAR-2), the reporting quality with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA 2020 Checkist), and the evidence quality according to the Grading of Recommendations Assessment, Development and Evaluation (GRADE) system. RESULTS: A total of 7 SRs/MAs were included. According to AMSTAR-2, all the SRs/MAs included were categorized as critically low-quality. According to the PRISMA 2020 checklist, none of the reviews completed all the 27 items, thus their compliance was relatively weak. On the base of GRADE system, 2 of the 12 outcomes were rated as moderate, and 5 outcomes were rated as low-quality, while the others were regarded as very low-quality. CONCLUSION: Compared with the control group, the included reviews of the acupuncture group were more effective and safer; however, the conclusion should be treated cautiously because the quality of evidence was not high enough to support it. In order to improve the quality, more rigorous, standardized, and comprehensive SRs/MAs need designing in the future.

Maturation of Nephrons by Implanting hPSC-derived Kidney Progenitors Under Kidney Capsules of Unilaterally Nephrectomized Mice.

BACKGROUND: Human pluripotent stem cell (hPSC)-derived kidney organoids may contribute to disease modeling and the generation of kidney replacement tissues. However, the realization of such applications requires the induction of hPSCs into functional mature organoids. One of the key questions for this process is whether a specific vascular system exists for nephrogenesis. Our previous study showed that short-term (2 weeks) implantation of hPSC-derived organoids below the kidney capsules of unilaterally nephrectomized and immunodeficient mice resulted in the enlargement of organoids and production of vascular cells, although signs of maturation were lacking. METHODS: Organoids were induced for 15 days in vitro and then grafted below kidney capsules of the same unilaterally nephrectomized immunodeficient mouse model to examine whether medium-term (4 weeks) implantation could improve organoid maturation and vascularization, as evaluated by immunofluorescence and transmission electron microscopy. RESULTS: We demonstrated that after 2-4 weeks of implantation, renal organoids formed host-derived vascularization and matured without any exogenous vascular endothelial growth factor. Glomerular filtration barrier maturation was evidenced by glomerular basement membrane deposition, perforated glomerular endothelial cell development, and apical, basal podocyte polarization. A polarized monolayer epithelium and extensive brush border were also observed for tubular epithelial cells. CONCLUSIONS: Our results indicate that the in vivo microenvironment is important for the maturation of human kidney organoids. Stromal expansion and a reduction of nephron structures were observed following longer-term (12 weeks) implantation, suggesting effects on off-target cells during the induction process. Accordingly, induction efficiency and transplantation models should be improved in the future.

MOF-derived nitrogen-doped carbon-based trimetallic bifunctional catalysts for rechargeable zinc-air batteries.

Zinc-air battery (ZAB) is a promising new metal-air energy system, but the large overpotentials of oxygen evolution reaction (OER) and oxygen reduction reaction (ORR) around the air electrode lead to their poor energy efficiency. Herein, a novel bifunctional oxygen electrocatalyst is reported with the preparation of a zeolite imidazolate framework (ZIF-67) derived trimetallic composites decorated nitrogen-doped carbon, which consist of NiFe alloy and Co nanoparticles. The ZIF-derived porous N-doped carbon shell can speed up the mass transfer efficiency. Whereas the electronic effect between the formed NiFe alloy and Co nanoparticles, as well as the N-doped carbon framework can enrich the active centers and enhance the electrical conductivity. As a result, the NiFe-Co@NC-450 catalyst shows superior performance manifested as a small potential gap (ΔE = 0.857 V) between the overpotential at 10 mA cm-2(Ej=10) for OER (460 mV) and half-wave potential (E1/2) for ORR (0.833 V). The liquid ZABs exhibit a high specific capacity reaching 798 mAh/gZnand a stable cycling performance at 10 mA cm-2for more than 200 h. Meanwhile, the NiFe-Co@NC-450 based flexible ZABs also presents robust flexibility and stability. This study has certain implications for the development of economical, powerful and stable bifunctional catalysts for ZABs.

Coordinated Co-NC/CoFe dual active centre embedded three-dimensional ordered macroporous framework as bifunctional catalyst for efficient and stable zinc-air batteries.

Developing efficient and stable multifunctional electrocatalyst is very important for zinc-air batteries in practical. Herein, semiconductive spinel CuFe2O4supported Co-N co-doped carbon (Co-NC) and CoFe alloy nanoparticles were proposed. In this strategy, the three-dimensional ordered macroporous CuFe2O4support provides rich channels for mass transmission, revealling good corrosion-resistance and durability at the same time. ZIF-67 derived Co-NC decoration improves the conductivity of the catalyst. Further, the uniformly distributed Co-NC and CoFe nanoparticles (C/CF) dramatically promote the oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) performance. Accordingly, C/CF@CuFe2O4catalyst shows remarkable bifunctional electrocatalytic activity, with an ORR half-wave potential of 0.86 V, and an OER over-potential of 0.46 V at 10 mA cm-2. The zinc-air battery using this catalyst exhibits a power density of 95.5 mW cm-2and a durable cyclability for over 170 h at a current density of 10 mA cm-2, which implies a great potential in practical application.

A conductive and ordered macroporous structure design of titanium oxide-based catalytic cathode for lithium-sulfur batteries.

The application of lithium-sulfur (Li-S) batteries is hindered by the insulating characteristic of sulfur and slow reaction kinetics of lithium polysulfides. Here, we propose a three-dimensionally ordered macroporous (3DOM) structured conductive polar Ta-doped TiO2framework with supported Co active site (CoTa@TiO2) to enhance the conversion kinetics of polysulfides. The 3DOM structure serves as an efficient sulfur host for the active sulfur through abundant pores and adsorption site. At the same time, the macropores can buffer the volume expansion of sulfur and enlarged mass transfer. The strong electrostatic attraction between Ti-O bond and polysulfide also promotes the adsorption of polysulfides. Moreover, the doped-Ta improves the conductivity of TiO2by narrowing the band gap, whereas the supported Co can accelerate the catalytic transformation. Benefited from advanced structural design and synergistic effect of Co and Ta doped TiO2,the Li-S cell with 3DOM CoTa@TiO2cathode exhibits an impressive areal capacity of 3.4 mAh cm-2under a high sulfur loading of 5.1 mg cm-2. This work provides an alternative strategy for the development of carbon-based cathode materials for Li-S batteries.

Conductive Al-Doped ZnO Framework Embedded with Catalytic Nanocages as a Multistage-Porous Sulfur Host in Lithium-Sulfur Batteries.

Lithium-sulfur (Li-S) batteries possess many practical challenges including the lithium polysulfide (LiPS) "shuttle effect" and their sluggish conversion kinetics. To address these issues, a unique hierarchical porous architecture, combining highly conductive ordered macroporous skeleton and embedded microporous particles is rationally designed as a dual-effective polysulfide immobilizer and conversion promoter. In this nanoporous architecture, Al-doped ZnO (AZO) acts as a conductive macroporous framework, profiting chemical anchoring of LiPS as well as facilitating electrolyte infiltration and ion diffusion; Co nanoparticle-anchored N-doped carbon (Co-NC) derived from CoZn-metal-organic framework is embedded in the macropores to further strengthen the LiPS adsorption, catalytically accelerating conversion kinetics of LiPS simultaneously. Consequently, the Co-NC@AZO/S cathode delivers a notable rate capability of 635.5 mA h g-1 at 5 C. A high area capacity of about 5.8 mA h cm-2 with a mass loading of 6.8 mg cm-2 is also achieved under a lean electrolyte (E/S = 5.7). Additionally, the Li-S pouch cells equipped with Co-NC@AZO can be extended to sulfur loading as high as 4.0 mg cm-2, delivering a superb capability of 897.5 mA h g-1 after 100 cycles. This work puts forward a design for stably cycled and practically viable Li-S batteries.

Tape stripping and lipidomics reveal skin surface lipid abnormity in female melasma.

The skin barrier of melasma is involved in the pathogenesis of melasma. Previous studies have shown that there are differences in the expression of epidermal lipid genes in melasma, but little is known about the epidermis lipid composition of melasma. Compared with the non-lesional skin, the content of total lipids, phosphatidic acid, phosphatidylserine, and ceramide (Cer) increased significantly in the lesional skin. Multivariate data analysis indicated that 40 individual Cer lipid species were responsible for the discrimination. In terms of acyl chain length in Cer, the expressions of very long chain (VLC) (C20-C26) and ultra-long chain (ULC) (>C26) increased significantly in the lesional skin. However, Cer[AH] had negative correlations with the activation of melanocytes in the lesional skin. Some lipid species had lower expression in lesional skin with high activation of melanocytes, as well as the high darkness. The epidermal thickness of lesional skin was higher compared with the non-lesional skin. These results suggest that Cer increased significantly in the lesional skin of melasma, possibly as a compensatory mechanism to maintain skin barrier function. Between different groups of darkness and activation of melanocytes, the change of ceramides might have correlation with the pigmentation progress of melasma.

In vitro induction and in vivo engraftment of kidney organoids derived from human pluripotent stem cells.

The shortage of transplantable organs impedes the development of tissue-engineered alternatives. Producing tissues similar to immature kidneys from pluripotent stem cells is possible in vitro, but the size of the organoids is limited. Furthermore, in vivo implantation is necessary for organoid development and functional maturation. In the present study, the induction procedure was optimized and kidney organoids derived from induced pluripotent stem cells in vitro were produced. The kidney organoids were examined by immunofluorescence and quantitative PCR. Then, a unilateral nephrectomy model was established that was beneficial to the compensatory proliferation of the other kidney. Finally, these organoids were implanted below the kidney capsules of immunodeficient mouse hosts that had been nephrectomized unilaterally. This implantation resulted in the enlargement of the organoids and the production of vascular cells. Although signs of organoid maturation were lacking in short-term culture in vivo, the present study provided a method for studying kidney organoid development in vivo.

Low-Bandgap Se-Deficient Antimony Selenide as a Multifunctional Polysulfide Barrier toward High-Performance Lithium-Sulfur Batteries.

The shuttling behavior and sluggish conversion kinetics of the intermediate lithium polysulfides (LiPSs) represent the main obstructions to the practical application of lithium-sulfur (Li-S) batteries. Herein, an anion-deficient design of antimony selenide (Sb2 Se3- x ) is developed to establish a multifunctional LiPS barrier toward the inhibition of polysulfide shuttling and enhancement of battery performance. The defect chemistry in the as-developed Sb2 Se3- x promotes the intrinsic conductivity, strengthens the chemical affinity to LiPSs, and catalyzes the sulfur electrochemical conversion, which are verified by a series of computational and experimental results. Attributed to these unique superiorities, the obtained LiPS barrier efficiently promotes and stabilizes the sulfur electrochemistry, thus enabling excellent Li-S battery performance, e.g., outstanding cyclability over 500 cycles at 1.0 C with a minimum capacity fading rate of 0.027% per cycle, a superb rate capability up to 8.0 C, and a high areal capacity of 7.46 mAh cm-2 under raised sulfur loading. This work offers a defect engineering strategy toward fast and durable sulfur electrochemistry, holding great promise in developing practically viable Li-S batteries as well as enlightening the material design of related energy storage and conversion systems.

[The Expression of microRNA-221 in Endometriosis and Its Impact on Endometrial Stromal Cells].

OBJECTIVE: To determine the expression of microRNA-221 (miR-221) in endometrial tissues and its impact on the proliferation of ectopic endometrial stromal cells. METHODS: Endometrial stromal cells were isolated, cultured and identified from normal endometrial tissues (taken from patients without endometriosis) and ectopic endometrial tissues (taken from patients with ovarian endometriosis). The expression of microRNA-221 was detected by stem-loop qRT-PCR. Changes in the expression of miR-221-3p in endometrial stromal cells exposed to estraldiol (10-8 mol/L) for 48 h were detected. The effects of miR-221-3p inhibitor on the expressions of miR-221-3p, phosphatase and tensin homology deleted on chromosome ten (PTEN) and cell proliferations were compared with those of the negative control (NC, 10 nmol/L). RESULTS: The expression of miR-221-3p in ectopic endometrial tissues was 4.2 times higher than that in normal endometrial tissues (P=0.039): 2.66 times higher in ectopic endometrial stromal cells compared with normal endometrial stromal cells (P=0.029). But no differences in the expression of miR-221-5p were found (P>0.05). No differences in the change of miR-221-3p expression after exposure to estrogen for 48h were found between normal and ectopic stromal cells. Inhibition of miR-221-3p function was associated with decreased cell proliferation (P=0.018) and increased expression of PTEN gene (P=0.021). CONCLUSION: The expression of microRNA-221 is upregulated in ectopic endometrial tissues and ectopic endometrial stroma cells. Inhibiting the function of miR-221-3p may result in increased PTEN expression and decreased cell proliferation in endometrial stromal cells.

Manipulating Depletion Region of Aqueous-Processed Nanocrystals Solar Cells with Widened Fermi Level Offset.

Water soluble nanocrystals (NCs) are promising materials in aqueous-processed solar cells because of their high extinction coefficient, low-cost, and favorable photoelectric characteristics. However, the power conversion efficiency (PCE) of the present aqueous-processed NC solar cells is restricted by the short depletion region of the active layer and limited Fermi level offset between NCs and the electron transport layer. Herein, these issues are effectively addressed by preparing Cdx Zn1- x Te NCs capped with 2-aminoethanethiol hydrochloride. The introduction of Zn2+ into CdTe NCs widens the Fermi level offset from 0.68 to 0.74 eV, lengthens the depletion region from 130 to 137 nm, and hence brings obvious improvement in the open circuit voltage (Voc ) and fill factor. Especially, the depletion region is successfully tuned from 137 to 171 nm, and even lengthened to a record thickness of 200 nm based on aqueous-processed solar cells. As a result, a champion thickness ratio (74%) of depletion region to active layer (200/270 nm) is achieved. A champion PCE of 5.96% and short-circuit current (Jsc ) of 21.2 mA cm-2 are achieved among aqueous-processed NC solar cells. This work provides a simple way to prepare polynary NCs and highlights a prospective method to develop more efficient and cost-effective solution-processed environment friendly solar cells.

Recellularization of well-preserved decellularized kidney scaffold using adipose tissue-derived stem cells.

To establish a recellularization kidney model by using adipose tissue-derived stem cells (ADSCs) as seeding cells and to investigate the growth and differentiation of ADSCs in decellularized kidney scaffolds. ADSCs were isolated using a modified method and then identified using flow cytometry analysis. Osteogenesis and adipogenesis differentiation were performed. Rat kidneys were decellularized using 0.5% sodium dodecyl sulfate. Immunofluorescence, immunohistochemistry, and scanning electron microscope were conducted to examine the scaffold microstructure. The decellularized kidney scaffold was seeded with ADSCs antegrade through the artery or retrograde through the ureter and cultured for 5-10 days. Hematoxylin and eosin staining, immunofluorescence, and immunohistochemistry were applied to assess growth and differentiation of seeding cells within the scaffold. ADSCs populated within the glomerular, vascular, and tubular area of kidney scaffolds. Cells differentiated toward endothelial or tubular cells. Stromal cell-derived factor 1 promoted cell attachment in the scaffold. These findings suggest that ADSCs can be used as an additional new source of seeding cells within decellularized kidney scaffold. This combination may offer an alternative to donor kidney transplant. In this way, autologous ADSCs can be utilized as seeding cells in cell-scaffold kidney regeneration for further clinical transplantation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 805-814, 2018.

Construction of engineered corpus cavernosum with primary mesenchymal stem cells in vitro.

Various methods have been used to reconstruct the penis. The objective of this study was to investigate the feasibility of constructing engineered corpus cavernosum with primary mesenchymal stem cells (MSCs) in a rabbit model in vitro. Acellular corporal matrices (ACMs) were obtained from adult rabbit penile tissues through an established decellularization procedure. MSCs were separated, purified, and then seeded on ACMs to construct engineered corpus cavernosum. The seeded ACMs were subsequently cultured in an incubator for 14 days. Histological analyses showed that MSCs seeded on the ACMs had proliferated and were well distributed. Detection of CD31, vWF, smooth muscle actin (SMA), and myosin protein as well as vWF and myosin mRNA revealed that the MSCs had differentiated into endothelial cells and smooth muscle cells. In addition, cell morphology of the engineered corpus cavernosum was directly observed by transmission electron microscopy. This study demonstrated that engineered corpus cavernosum could be successfully constructed using primary MSCs in vitro. This technology represents another step towards developing engineered corpus cavernosum in vitro.

High-Efficiency Aqueous-Processed Polymer/CdTe Nanocrystals Planar Heterojunction Solar Cells with Optimized Band Alignment and Reduced Interfacial Charge Recombination.

Aqueous-processed nanocrystal solar cells have attracted increasing attention due to the advantage of its environmentally friendly nature, which provides a promising approach for large-scale production. The urgent affair is boosting the power conversion efficiency (PCE) for further commercial applications. The low PCE is mainly attributed to the imperfect device structure, which leads to abundant nonradiative recombination at the interfaces. In this work, an environmentally friendly and efficient method is developed to improve the performance of aqueous-processed CdTe nanocrystal solar cells. Polymer/CdTe planar heterojunction solar cells (PHSCs) with optimized band alignment are constructed, which results in reduced interfacial charge recombination, enhanced carrier collection efficiency and built-in field. Finally, a champion PCE of 5.9%, which is a record for aqueous-processed solar cells based on CdTe nanocrystals, is achieved after optimizing the photovoltaic device.