Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Mol Cell Biol ; 34(13): 2517-32, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24777602

ABSTRACT

MET, the receptor for hepatocyte growth factor (HGF), plays an important role in signaling normal and tumor cell migration and invasion. Here, we describe a previously unrecognized mechanism that promotes MET expression in multiple tumor cell types. The levels of the Pim-1 protein kinase show a positive correlation with the levels of MET protein in human tumor cell lines and patient-derived tumor materials. Using small interfering RNA (siRNA), Pim knockout mice, small-molecule inhibitors, and overexpression of Pim-1, we confirmed this correlation and found that Pim-1 kinase activity regulates HGF-induced tumor cell migration, invasion, and cell scattering. The novel biochemical mechanism for these effects involves the ability of Pim-1 to control the translation of MET by regulating the phosphorylation of eukaryotic initiation factor 4B (eIF4B) on S406. This targeted phosphorylation is required for the binding of eIF4B to the eIF3 translation initiation complex. Importantly, Pim-1 action was validated by the evaluation of patient blood and bone marrow from a phase I clinical trial of a Pim kinase inhibitor, AZD1208. These results suggest that Pim inhibitors may have an important role in the treatment of patients where MET is driving tumor biology.


Subject(s)
Eukaryotic Initiation Factors/genetics , Hepatocyte Growth Factor/metabolism , Proto-Oncogene Proteins c-met/biosynthesis , Proto-Oncogene Proteins c-pim-1/biosynthesis , Animals , Biphenyl Compounds/pharmacology , Cell Line, Tumor , Cell Movement , Eukaryotic Initiation Factor-3/genetics , HeLa Cells , Humans , Mice , Mice, Knockout , Neoplasm Invasiveness , Phosphorylation , Protein Binding , Protein Biosynthesis , Protein Kinase Inhibitors/pharmacology , Proto-Oncogene Proteins c-akt/genetics , Proto-Oncogene Proteins c-met/genetics , Proto-Oncogene Proteins c-pim-1/antagonists & inhibitors , Proto-Oncogene Proteins c-pim-1/genetics , RNA Interference , RNA, Small Interfering , Signal Transduction , Thiazolidines/pharmacology
2.
J Immunol ; 181(6): 4089-97, 2008 Sep 15.
Article in English | MEDLINE | ID: mdl-18768865

ABSTRACT

Steroid-resistant asthma comprises an important source of morbidity in patient populations. T(H)17 cells represent a distinct population of CD4(+) Th cells that mediate neutrophilic inflammation and are characterized by the production of IL-17, IL-22, and IL-6. To investigate the function of T(H)17 cells in the context of Ag-induced airway inflammation, we polarized naive CD4(+) T cells from DO11.10 OVA-specific TCR-transgenic mice to a T(H)2 or T(H)17 phenotype by culturing in conditioned medium. In addition, we also tested the steroid responsiveness of T(H)2 and T(H)17 cells. In vitro, T(H)17 cytokine responses were not sensitive to dexamethasone (DEX) treatment despite immunocytochemistry confirming glucocorticoid receptor translocation to the nucleus following treatment. Transfer of T(H)2 cells to mice challenged with OVA protein resulted in lymphocyte and eosinophil emigration into the lung that was markedly reduced by DEX treatment, whereas T(H)17 transfer resulted in increased CXC chemokine secretion and neutrophil influx that was not attenuated by DEX. Transfer of T(H)17 or T(H)2 cells was sufficient to induce airway hyperresponsiveness (AHR) to methacholine. Interestingly, AHR was not attenuated by DEX in the T(H)17 group. These data demonstrate that polarized Ag-specific T cells result in specific lung pathologies. Both T(H)2 and T(H)17 cells are able to induce AHR, whereas T(H)17 cell-mediated airway inflammation and AHR are steroid resistant, indicating a potential role for T(H)17 cells in steroid-resistant asthma.


Subject(s)
Bronchial Hyperreactivity/immunology , Bronchial Hyperreactivity/pathology , Dexamethasone/pharmacology , Drug Resistance/immunology , Interleukin-17/metabolism , Receptors, Interleukin-17/physiology , T-Lymphocyte Subsets/immunology , Th2 Cells/immunology , Animals , Bronchial Hyperreactivity/prevention & control , Cells, Cultured , Chemokines/metabolism , Chemotaxis, Leukocyte/immunology , Clone Cells , Disease Models, Animal , Lung/immunology , Lung/pathology , Male , Mice , Mice, Inbred BALB C , Mice, Knockout , Mice, SCID , Mice, Transgenic , Receptors, Interleukin-17/deficiency , Receptors, Interleukin-17/genetics , T-Lymphocyte Subsets/metabolism , T-Lymphocyte Subsets/transplantation , Th2 Cells/metabolism , Th2 Cells/transplantation
SELECTION OF CITATIONS
SEARCH DETAIL
...