Functional analysis of long-chain non-coding RNA in oral squamous cell carcinoma.

BACKGROUND: This study aimed to investigate the expression profile of long-chain non-coding RNA (lncRNA) in oral squamous cell carcinoma (OSCC) and to explore the biological functions of differentially expressed lncRNA in the cancer tissues as compared with adjacent normal tissues, and the differentially expressed lncRNAs related to OSCC were screened. METHODS: High-throughput lncRNA microarray assay was used to detect the expression of lncRNA and mRNA in the OSCC tissues and adjacent normal tissues from five patients. The expression profiles of the lncRNA and mRNA in the cancer tissues and adjacent normal tissues were analyzed and the differentially expressed lncRNA and mRNAs were identified. The differentially expressed mRNA was analyzed with GO, Pathway and disease annotation enrichment database, and the mRNAs related to the tumor and the lncRNA-mRNA co-expression network were employed to screen the key lncRNA related to the occurrence and development of OSCC. RESULTS: A total of 3,022 differentially expressed lncRNAs and 4,364 differentially expressed mRNAs were identified in the OSCC tissues as compared with adjacent normal tissues. A further analysis revealed 130 major differentially expressed mRNAs related to the tumor. When the correlation was >0.99 or <-0.99 and P value was <0.05, there were 73 differentially expressed mRNA in case of mRNA /lncRNA co-expression. The intersection of two gene symbols resulted in the nine lncRNAs closely related to the OSCC, in which five showed up-regulation and six had down-regulation. Based on the co-expression of the lncRNAs and mRNAs (correlation 40.99 or correlation -0.99 and P value <0.05), there were differentially expressed mRNAs with co-expression with lncRNA. CONCLUSIONS: The differentially expressed lncRNAs identified in this study are related to the occurrence and development of OSCC. This may provide new therapeutic targets and biomarkers for OSCC and is also helpful for further investigation of pathogenesis of OSCC.

[The value of single photon emission computed tomography-computed tomography in diagnosis of carcinoma invasion of mandible].

OBJECTIVE: To investigate the value of single photon emission computed tomography(SPECT)-computed tomography(CT) in diagnosis of oral carcinoma invasion to mandible. METHODS: Thirty-four patients with oral carcinoma invasion to mandible were divided into two groups, group A (surrounding invasion) and group B (central invasion). The edge of the invasion was evaluated by SPECT-CT, CT and pathological examination. The results of CT and SPECT-CT were analyzed by quantitative methods. RESULTS: In group A, the cancer-invaded area of the mandible exhibited on SPECT-CT was 1.0 cm lager than that on pathological examination, 2.4 cm lager than that on CT. The difference of invaded area shown on CT was 1.4 cm smaller than that of pathological examination. There were significant difference among the three methods. In group B, the affected area on SPECT-CT was 1.2 cm lager than that of pathological examination, 4.2 cm lager than that of CT. The invision area on CT was 3.0 cm smaller than that of pathological examination. There were significant difference among the three methods. CONCLUSIONS: SPECT-CT could find the jaw central tumor earlier than CT and the range of lesion showed by SPECT-CT was the adequate range of bone incision during operation. The range of lesion showed by CT was influenced by the type of tumor and the range of bone incision was determined according to the pathological type. If the false negative result was eliminated, only SPECT-CT or CT was needed to estimate the invasion range of mandible.

[A comparison between CT and SPECT in detecting mandibular invasion by lower gingival squamous cell carcinoma].

PURPOSE: The purpose of this study is to compare the value of CT and SPECT in diagnosis of lower gingival carcinoma invading the mandible. METHODS: From February 2002 to October 2006, twenty-one patients with lower gingival squamous cell carcinoma were enrolled.The data of CT and SPECT were studied,and compared with histopathological findings. RESULTS: Among the 21 patients,the sensitivity, accuracy, negative predictive value and Youden's index of SPECT were 100.00%, 95.24%, 100.00% and 1.00,respectively. While the sensitivity, accuracy, negative predictive value and Youden's index of CT were 80.00%, 80.95%, 20.00% and 0.80, respectively. There were four false negatives assessments of bone invasion(80.00%) by CT scan, while no false negatives by SPECT. CONCLUSIONS: SPECT is superior to CT, and can be used as a routine screening method to assess lower gingival carcinoma invading the mandible.