ABSTRACT
This study focuses on the determination of iodine content in healthy thyroid samples on male population from Caracas, Venezuela. Contribution to establish a baseline of iodine content in thyroid glands and hence to compare the iodine thyroid concentration of the Venezuelan population with other countries is also our objective. Male post-mortem individual samples were analyzed using a spectrophotometric flow injection method, based on the Sandell-Kolthoff reaction. The median intrathyroidal iodine concentration was 1443+/-677 microg/g (wet weight), ranging from 419 to 3430 microg/g, which corresponds to a median of total iodine content of 15+/-8 mg (ranging from 4 to 37). These results were higher than those values reported in the literature. No correlation of iodine content with age or weight of the healthy gland was found.
Subject(s)
Iodine/analysis , Thyroid Gland/chemistry , Adolescent , Adult , Humans , Male , Middle Aged , Population Groups , Spectrophotometry , VenezuelaABSTRACT
This study focuses on the determination of selenium and iodine in human thyroids. The glands were digested using nitric acid in a microwave oven. Selenium was determined by inductively coupled plasma optical emission spectrometry (ICP-OES) using a new sample introduction system consisting of a reduction system coupled to a hydride generation nebulizer (DHGN). Iodine was determined by using the Sandell-Kolthoff procedure. The detections limits were 0.2 ng/mL and 0.3 ng/mL for the determination method of selenium and iodine, respectively. The amount of iodine in the whole gland was 3.44 +/- 1.11 microg/g. The lowest iodine level was 2.34 microg/g and the highest 5.21 microg/g. The lowest selenium concentration for a single sample was 505 +/- 51 ng/g and the highest 1495 +/- 204 ng/g depending on the fraction of the gland selected.
Subject(s)
Iodine/analysis , Selenium/analysis , Thyroid Gland/chemistry , Humans , Oxidation-Reduction , Reproducibility of Results , Sensitivity and Specificity , Spectrophotometry/instrumentation , Spectrophotometry/methods , Thyroid Gland/anatomy & histologyABSTRACT
Estudiamos 325 historias clínicas de pacientes con enfermedad de la tiroides que asistieron al servicio de endocrinología del Hospital Vargas y comparamos el diagnóstico inmunológico de tiroidítis crónica(TC) establecido por anticuerpos antiperoxidasas (AAP) con el estudio cito histológico del material obtenido por la biopsia por punción con aguja fina y gruesa (BPAFG) de la tiroides, demostrándose una sensibilidad del 62 por ciento y una especificidad del 96,7 por ciento del método citado en primer lugar. La relación entre el diagnóstico funcional de la tiroides por clínica determinación hormonal evidenció una sensibilidad 47 por ciento y una especificidad del 96,7 por ciento del primer método. La determinación de los AAP ayuda y complementa el diagnóstico definitivo de TC. El diagnóstico clínico funcional de la tiroides debe ser corroborado con el estudio hormonal
Subject(s)
Humans , Antibodies , Biopsy , Histocytological Preparation Techniques , Thyroiditis, AutoimmuneABSTRACT
Se presenta un caso de hipoparatiroidismo secundario a tiroidectomía y como complicación iatrogénica se produjo paratiroidectomía. Se trata de una paciente, femenino, de 47 años de edad, hospitalizada por convulsiones, cuya sintomatología inherente a hipocalcemia se había iniciado desde la edad de 30 años, cuando se le practicó tiroidectomía por causa que no precisa. Por tomografía axial computarizada de cráneo se evidenció la presencia de calcificaciones en los ganglios basales
