ABSTRACT
This work describes the first molecular characterization of grapevine virus B (GVB) in Portuguese grapevine cultivars. During a routine screening of 44 accessions in the National Collection of Grapevine Varieties (CAN PRT051), 17 were found infected with GVB in DAS-ELISA assays with commercial antibodies. However, only six of the corresponding isolates were successfully amplified using primer pairs described in the literature. The sequence variants (ORF4-3'UTR, 1147 nt) retrieved from these isolates segregated into two phylogenetic groups, which included sequences from complete genomes available in GenBank. The highly discrepant results obtained using serological and RT-PCR-based diagnostic tools led to the design of a primer pair for detection of GVB, which allowed the amplification of a 606-bp GVB-specific fragment from all DAS-ELISA-positive isolates and also revealed the existence of false negatives in the serological testing.
Subject(s)
Flexiviridae/isolation & purification , Genetic Variation , Reverse Transcriptase Polymerase Chain Reaction/methods , Virology/methods , Vitis/virology , DNA Primers/genetics , Flexiviridae/classification , Flexiviridae/genetics , Open Reading Frames , Phylogeny , Plant Diseases/virology , Portugal , Sequence Analysis, DNAABSTRACT
O estudo retrospectivo de 154 casos de AIDS neuropsiados no Hospital Universitário Antonio Pedro (HUAP) mostraram lesöes no Sistema Nervoso Central. A toxoplasmose cerebral foi a lesäo neurológica mais comum, achada em 60 casos (38,9 por cento). Demos ênfase aos dados clínicos e epidemiológicos, tempo de evoluçäo, achados neuropatológicos neurorradiológicos e sorológicos, com o objetivo de obter orientaçäo para diagnóstico precoce e terapêutica, permitindo aumento da sobrevivência dos pacientes com AIDS e toxoplasmose.
