[Study of the interaction of 2'-5'-oligoadenylates and their analogues with proteins by fluorescence spectroscopy].

The interaction of "core" 2'-5'-oligoadenylates (2-5A) and their analogues with proteins albumin, interferon and immunoglobulin G was studied by fluorescence spectroscopy methods. Strong quenching of protein fluorescence (up to 67%) was observed upon interaction of oligoadenylates in concentration of 5 x 10(-5) M with albumin. Investigated compounds quenched the emission of interferon to a lesser extent, whereas no significant fluorescence changes occurred upon interaction with immunoglobulin under the same conditions. The level of quenching depended on the structure of 2-5A compounds and decreased with the decrease of their concentration. These data suggest that 2-5A actively bind to albumin and less efficiently to interferon, and practically do not interact with immunoglobulin. Taking into consideration different efficiency of quenching of the fluorescence excited at 280 and 296 nm, the assumption has been made about a possible role of tyrosine and tryptophan in the binding of a given compound to proteins. Possible mechanisms of interaction of oligoadenylates with proteins are discussed.

[Primary hypersplenism due by accessory spleens after splenectomy]. / Hipersplenism trombocitopenic prin spline accesorii dupa splenectomie.

Primary hypersplenism may affect any of the figurative elements of blood, simultaneously or in successive sequences. In function the answers, the therapy may be necessary splenectomy. The authors present the case of a 50-year-old patient what suffered one splenectomy, admitted to the 1st Surgical Clinic for purpuric cutaneous phenomena. 32 years ago, the patient suffered the splenectomy, from a severe autoimmune hemolytic anemia. After 29 years the biologic exam realized for a generalized purple, relieved a severe thrombocytopenia (2,500/mm3) being necessary the administration of thrombocytes transfusion and high doses of corticosteroids in emergency. The spleen sequestration test, ultrasound exam and CT showed the existence of accessory spleens. We practice the surgical intervention for ablation of the accessory spleens. The splenectomy cure disease in 65-80% of cases and the partial remission of manifestation is obtained in 15-20% of cases. The recurrence risk is possible by omission of the accessory spleens. The ablation of these accessory spleens induced recovery in 98-99% of cases.

[Minimally invasive treatment of pulmonary and pleural hydatid cyst]. / Tratamentul minim invaziv al chistului hidatic pulmonar si pleural.

The incidence of hydatid disease is increased in Balkan region and Eastern Europe. The global incidence in Romania is 5-6 cases of 100,000 populations. The etiological agent is Taenia echinococcus. The pulmonary localization is about 30% from all cases with hydatic disease. The only efficient treatment is represented by surgical intervention with minimal visceral and parietal destruction. We will present 4 cases with pulmonary and pleural hydatic cysts that were treated with minimally invasive techniques. New minimally invasive approaches are developed to reduce physical discomfort, to offer a better intraoperative visibility, for the esthetic aspect of incisions, shorten hospitalization and quick socio-professional reintegration. The mean duration of hospitalization in these 4 cases was 8.5 days (limits between 7-12 days). The rate of complication and the necessary of analgesics were reduced. The medical treatment with Albendazole 10 mg/kg/day, 14 days in preoperative and 3 months postoperative period was followed in all patients. After discharge, imaging evaluation in the first 24 months is very important. The obtained results were excellent and we conclude that minimally invasive surgery in pulmonary hydatid disease represent a better method of surgical treatment.

[Jejunoileal bypass]. / By-pass-ul jejunoileal.

Obesity is a condition which can be found very frequently today, both in developed and 3rd world countries. The incidence of obesity in adult population of Romania is about 35%, and most of these patients are females. We'll present the case of a 54 years old woman with BMI = 57 kg/m2, who was hospitalized for the treatment of a postoperative eventration after an umbilical hernia. Her nocturnal breathing troubles, knee pains and walking difficulties made us consider the idea of a digestive by-pass. The surgical intervention consisted of jejunoileal by-pass, abdominoplasty and dermolipectomy with bipolar drainage. Many complications occurred in the postoperative period (renal failure due to severe diarrhea). The weight loss after 18 months was 37%, which means 66% of the weight surplus (similar results can be found in professional statistics--around 70%). After 18 mounts her weight is 95 kg and she allowed to consume any food. 18 mounts after the operation, the number of stools decreased to normal (1-2 per day). In conclusion the morbid obesity can and must be treated surgically. Jejunoileal by-pass is a highly effective procedure, but surgeons must be aware of the pact that severe complications which may occur anytime and must be treated immediately. After this kind of operation, weight stabilization can be achieved within 2 years, no diet being necessary as an additional treatment.