1.
Chem Commun (Camb)
; 51(57): 11482-4, 2015 Jul 21.
Article
in English
| MEDLINE
| ID: mdl-26091539
ABSTRACT
Recombinant proteins bearing a tag are crucial tools for assessing protein location or function. Small tags such as Cys4 tag (tetracysteine; Cys-Cys-X-X-Cys-Cys) are less likely disrupt protein function in the living cell than green fluorescent protein. Herein we report the first example of the design and synthesis of a dual fluorescence and hyperpolarized (129)Xe NMR-based sensor of Cys4-tagged proteins. This sensor becomes fluorescent when bound to such Cys4-tagged peptides, and the (129)Xe NMR spectrum exhibits a specific signal, characteristic of the biosensor-peptide association.
Subject(s)
Cysteine/analysis , Fluoresceins/chemistry , Fluorescent Dyes/chemistry , Organometallic Compounds/chemistry , Peptides/analysis , Polycyclic Compounds/chemistry , Amino Acid Sequence , Biosensing Techniques , Molecular Sequence Data , Nuclear Magnetic Resonance, Biomolecular , Recombinant Proteins/analysis , Spectrometry, Fluorescence , Xenon Isotopes/chemistry
2.
Rev Epidemiol Sante Publique
; 51(6): 649-52, 2003 Dec.
Article
in French
| MEDLINE
| ID: mdl-14967997
Subject(s)
Antineoplastic Agents/economics , Home Care Services, Hospital-Based/economics , Hospitalization/economics , Antineoplastic Agents/therapeutic use , Cost-Benefit Analysis , Female , France , Health Personnel/economics , Humans , Male , Middle Aged , Neoplasms/drug therapy , Prospective Studies
3.
J Radiol Electrol Med Nucl
; 52(3): 181-4, 1971.
Article
in French
| MEDLINE
| ID: mdl-4930891
