[Method of automated cytomorphological analysis for classifying leukocytes by their stage of destruction]. / Metod avtomatizirovannogo tsitomorfologicheskogo analiza dlia klassifikatsii leikotsitov po stadiiam destruktsii.

Destruction of human peripheral blood leukocytes by toxins has been studied with the aid of an automatic microimage analyzer the "Morphoquant". The most informative parameters which define destruction stages have been revealed: the area and number of fragments of nucleus images and their distance to the nucleus centre under different levels of the optical threshold. Software for morphological analysis of leukocytes has been developed in addition to their classification using four destruction stages. The results of computerized classification of blood smears will compare with those obtained by a working haemotologist (85% coincidence).

[Patterns in the body's immune response to administration of El Tor vibrio cholera toxin to experimental animals]. / Nekotorye zakonomernosti immunnoi reaktsii organizma na vvedenie èksperimentalnym zhivvotnym toksina kholernogo vibriona El Tor

A study was made of some regularities attending the mechanisms of antibody production in the organism of experimental animals after the administration of El Tor cholera vibrio toxin. As revealed, the indices of the immune response depended on the chosen model, the method of its determination, the method of administration and the amount of the toxin administered. Dynamics of the primary and secondary responses of the immunocompetent cells of mouse spleen was determined. With the action of the El Tor toxin on the lymphoid tissue of mice there were expressed the general biological regularities of the response -- the optimal amount of the antigen-antibody complexes caused activation of the antibody-producing cells; high doses depressed the most reactive immunocompetent Y-cells. Apparently, there existed a genetic determinination of the immune response of the organism to a definite antigen -- the toxin of the El Tor cholera vibrio. It is supposed that it is necessary to choose groups which mostly require cholera vaccination.