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5.
Z Naturforsch C Biosci ; 30(5): 562-4, 1975.
Article in English | MEDLINE | ID: mdl-175596

ABSTRACT

Nicotinamide-(S-methylmercury-thioinosine) dinucleotide was formed by reaction of nicotinamide-(6-thiopurine) dinucleotide with methylmercury chloride. The compound exhibits coenzyme properties in the test with LDH (Km=1.5 X 10(-4) M, Vmax=12500) and LADH (Km=1.7 X 10(-4) M, Vmax=27) and inactivates YADH and GAPDH. From incubations with LDH and LADH the mercury containing coenzyme could be regarded by column be qualified for the X-ray structure analysis of the coenzyme-enzyme complex for some dehyrogenases based on the proportion of the heavy metal.


Subject(s)
Alcohol Oxidoreductases/metabolism , Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism , L-Lactate Dehydrogenase/metabolism , NAD/analogs & derivatives , Organometallic Compounds/metabolism , Sulfhydryl Compounds/metabolism , Coenzymes , Kinetics , NAD/chemical synthesis , NAD/metabolism , Organometallic Compounds/chemical synthesis
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