ABSTRACT
To determine the effects of ozone on the phagocytosis of bovine polymorphonuclear leukocytes (PMNs), ozone gas was administered in vitro on the blood and milk of healthy lactating cows, cows with acute mastitis, and cows with milk fever. In the blood of healthy dairy cattle, although there was no significant effect of ozone gas on the viability of the leukocytes, phagocytosis of PMNs significantly decreased. In contrast, ozone gas administration in vitro significantly increased phagocytosis of PMNs from the blood of cows with acute mastitis and milk fever, and from mastitic milk. These findings showed that ozone administration in vitro has positive and negative effects on bovine PMN phagocytosis, depending on the health status of the animal.
Subject(s)
Milk/cytology , Neutrophils/drug effects , Oxidants, Photochemical/pharmacology , Ozone/pharmacology , Phagocytosis/drug effects , Animals , Anti-Bacterial Agents/therapeutic use , Cattle , Cattle Diseases/blood , Cattle Diseases/immunology , Cells, Cultured , Female , Kanamycin/therapeutic use , Mastitis, Bovine/blood , Mastitis, Bovine/drug therapy , Mastitis, Bovine/immunology , Neutrophils/immunology , Parturient Paresis/blood , Parturient Paresis/immunology , Penicillins/therapeutic use , Pregnancy , Streptomycin/therapeutic useABSTRACT
The prevalence of Cryptosporidium infection was examined in 480 healthy cattle (0-39 months old) in the Tokachi district in Hokkaido during the period from June to September in 2000 and from June to July in 2001. C. parvum oocysts were detected in 6 of 50 cattle (0-2 months old) in 2001; while C. muris was detected in 2 of 56 cattle (6-8 months old) in 2001, in 1 of 15 cattle (9-11 months old) in 2001, in 1 of 88 cattle (15-17 months old) in 2000, in 4 of 89 cattle (18-21 months old) in 2000 and in 2 of 53 cattle (21-23 months old) in 2000.
