[Role of non-haem iron in protecting effect of ecdysterone on development of streptozocin-induced hyperglycaemia in rats].

Chronic hyperglycaemia (60 days) which developed after streptozotocine (STZ) administration (5 mg/100 g) in rats was accompanied with development of severe endothelial dysfunction as well as with disturbed non-haem iron metabolism. It was established by EPR spectroscopy method that STZ administration reduced transferrin levels in the blood as well as pools of iron associated with blood transferrin and with ferritin in the heart and aorta of rats with hyperglycaemia. Chronic ecdysterone administration (100 ng/100 g, 60 days) protects hyperglycaemia development by preventing of non-haem iron metabolism disturbance. These data suppose participation of non-haem iron in mechanisms of ecdysterone protection of streptozotocine-induced hyperglycaemia and ischemia.

Pharmacokinetics of the doxorubicin magnetic nanoconjugate in mice. Effects of the nonuniform stationary magnetic field.

Pharmacokinetics of the doxorubicin (DOX) conjugates with magnetite nanoparticles of the core/ shell type in mice following i.v. injection in a dose of 12.5 microg Fe/g tissue w/w was studied using electron spin resonance technique (ESR). Conjugation of the DOX with magnetic nanoparticles was shown to considerably decrease DOX bioavailability in the heart and kidney tissues compared to the free DOX. A non-uniform stationary magnetic field B of 210 mT and [deltaB] of 200 mT/cm was found to be efficient in increasing DOX conjugate bioavailability in the target site. The magnetic field was also found to inhibit conjugate accumulation in the liver resulted in the increased bioavailability of the conjugates in the blood. The phenomenon can be associated with in vivo inhibition of the phagocytic activity of the immunocompetent cells upon application of magnetic fields. Morphometry data in agreement with pharmacokinetic data revealed a decrease in the conjugate concentration in the liver tissue and cells as well as the relative decrease in conjugate concentration in the Kupffer cells compared to hepatocytes upon application of magnetic fields.

Some signal effects in human erythrocytes on specific binding of free doxorubicin and doxorubicin conjugates with magnetite nanoparticles.

Binding of doxorubicin (DOX) immobilized on nanodispersed magnetite (DOX-M conjugates with loading in the range of 0.16-25.1 mg DOX/g carrier) to intact human erythrocytes in concurrence with free DOX was investigated. Two specific binding sites for doxorubicin were revealed at the plasma membrane of human erythrocytes. Changes in the ordering of the DOX-M nanoparticles according to small angle scattering data are consistent with their specific binding at the plasma membrane upon incubation with erythrocytes. Free and conjugated doxorubicin modulated signal transduction in erythrocytes in a similar way. Both up-regulate nitric oxide and cyclic GMP and down-regulate cyclic AMP production and stabilize the membranes of oxidatively damaged erythrocytes.

[Effect of alpha-lipoic acid preparations on bilirubin and transferrin levels and ferritin iron and transferrin iron content]. / Vplyv preparativ alpha-lipoievoï kysloty na riven' bilirubinu, transferinu ta vmist azliza u skladi ferytinu i transferynu.

The influence of ethylendiamine salt of alpha-lipoic acid on the indices of iron metabolism in patients with occupational pathologies has been studied using quantitative electron spin resonance spectroscopy. In the cases of treatment in the patients suffering from hyperferremia the decrease in transferrin iron concentration in the whole blood and plasma occurs correlating with the enhancement of iron excretion from urine and decline of bilirubin level in serum. We have found that the preparation chelates iron from iron (III)-citrate complex and form stable iron (III) complexes. The conclusion is that the positive effects of lipoic acid preparation in the patients with hyperferremia at least partially could be associated with normalization of iron exchange and reduction in the labile iron pool.

[Berlition R 300 oral -- alpha-lipoic acid preparation for the correction of body changes associated with high serum iron content]. / Preparat alfa-lipoevoi kisloty berlition R 300 oral' kak sredstvo korrektsii izmenenii v organizme, sviazannykh s giperferremiei.

Medication Berlition 300 oral, produced by "Berlin-Hemie", induces lower serum iron content, decrease in transferrin content of whole blood and serum proportionally to increased urinary iron excretion and lower serum bilirubin and normalizes serum glucose level. Berlition 300 oral, lipoic acid preparation made by "Berlin-He mie" (Menarini group), could be recommended for treatment of high serum iron content and iron overload, especially in association with diabetes mellitus.

[Concentration of transferrin iron and transferrin saturation in blood]. / Kontsentratsiia zaliza transferynu i stupin' nasychennia transferynu, vyznacheni v tsil'nii krovi.

Transferrin iron, transferrin protein concentrations, and transferrin saturation have been determined for the first time in the whole blood. Microsamples were taken from healthy adults and patients with occupational secondary haemochromatosis using quantitative electron spin resonance technique. At elevated transferrin saturation, transferrin saturation values determined in the plasma and serum samples were shown to be less than respective values determined in the whole blood of the same patients. At increased transferrin iron concentration the difference between experimental and reference data sets determined in the blood and plasma was statistically significant in contrast to data sets determined in serum. Therefore, the analysis of the blood microsamples ensured an adequate estimation of transferrin iron concentration, especially at high transferrin saturation. A new index--transferrin iron concentration in the formed blood elements--was introduced. The values of the index were determined in the groups of healthy adults, patients with secondary occupational hemochromatosis and healthy newborns.

[Quantification of chelatable iron in biological tissue by electron spin spectroscopy]. / Vyznachennia v biologichnykh tkanynakh kontsentratsiï zaliza, shcho khelatuiet'sia, metodom spektroskopiï elektronnoho spinovoho rezonansu.

The present paper deals with the procedure of a quantitative determination of chelatable iron in biological microsamples (20-50 mg) using an electron spin resonance technique based on the iron incorporation into the stable iron (III)-desferrioxamine B complexes. The conclusion is, that the method developed is valuable one to characterize the availability of iron from different sources. Using the method developed, the share of iron outside the protein core was shown to be less then 0.5 percentages of the whole iron content in the horse spleen ferritin suspension with 100 mg protein per ml and average iron content of 810 iron atoms per protein molecule. For a set of tissue samples taken from internal organs and brain of mice, rats and rabbits the data on chelatable iron concentration have been obtained.