ABSTRACT
The aim of our work was to determine the gene polymorphism of cytokines IL-1ß (-511) and IL-10 (-1082) in children with nephrotic syndrome. 20 patients with nephrotic syndrome were recruited into the study from 2017 to 2018 years in single center. Our study included children with levels of glomerular filtration rate >90 ml/min. Genetic polymorphism of IL-1ß (-511) and IL-10 (-1082) and serum IL1ß were evaluated. Analyzing the contents of IL-1ß in serum of children with nephrotic syndrome, we found that IL-1ß was significantly increased in children with steroid-resistant nephrotic syndrome and with progression of glomerulonephritis compared with remission and with healthy children (p<0.05). The presence of C/T genotype is associated with increased production of interleukin-1ß in serum, compared with children with genotype C/C (p<0.05). Checking the polymorphism of SNP -1082 of IL-10 we determined that in 50% of children with nephrotic syndrome there was G/A genotype, in 40% - G/G genotype, and genotype Ð/Ð was only in 10% of patients. A strong direct relationship between the level of IL-1ß in serum and C/T allelic polymorphism of the gene IL-1ß (-511) was found (r=+0,56) (p<0.05). Gene polymorphism of IL-1ß (-511) can be used as a marker of progression of glomerulonephritis, nephrotic syndrome but more studies are needed.
Subject(s)
Interleukin-10/genetics , Interleukin-1beta/genetics , Nephrotic Syndrome/diagnosis , Nephrotic Syndrome/genetics , Polymorphism, Genetic/genetics , Child , Female , Genetic Predisposition to Disease , Genotype , Humans , Interleukin-10/blood , Interleukin-1beta/blood , Male , Nephrotic Syndrome/immunologyABSTRACT
The problem of HBV and HCV infections in addition to the HIV-infection in sub-Saharan African countries remains important due to the high prevalence and mortality after fast progressing fibrogenesis and development of hepatocellular carcinoma. Despite of the large number of investigations on diagnostics and prediction of the disease course, the exact role of the proinflammatory influence of IP-10 and IL-17A on the fibrogenesis during HIV/HBV-co-infection is still unknown. The aim of the study was to investigate IP-10 and IL-17A concentration in blood serum among HIV/HBV patients to consider their potential role in improvement of diagnostics of liver fibrosis progression. 53 HIV/HBV patients of Lewanika General Hospital (West Zambia) and 21 healthy blood donors were checked for serological markers, liver biopsy and IP-10, IL-17A in blood serum. The obtained results were analyzed by statistical package SPSS 12.0. Mean IP-10 was 753,6 pg/ml among HIV/HBV co-infected patients with F3-4 and it was reliably higher than in F1-2 patients and healthy responders (Ñ=0,005). This group had also higher level of IL-17A (37,54 pg/ml) than comparison groups (Ñ=0,032). We found out strong correlation between increasing IP-10 (r=0,6), IL-17A (r=0,52) and fibrotic severity (Ñ<0,05). High IP-10, IL-17A amount increases the risk of F3-4 formation in HIV/HBV patients.
Subject(s)
Chemokine CXCL10/blood , Coinfection/epidemiology , HIV Infections/complications , HIV-1/isolation & purification , Hepatitis B/complications , Interleukin-17/blood , Liver Cirrhosis/etiology , Liver Cirrhosis/pathology , Liver/virology , Serum/virology , Adult , Biomarkers/blood , Biopsy , Case-Control Studies , Chemokine CXCL10/immunology , Disease Progression , Female , HIV Infections/blood , HIV Infections/epidemiology , Hepatitis B/blood , Hepatitis B/epidemiology , Hepatitis B virus , Humans , Interleukin-17/immunology , Liver/pathology , Liver Cirrhosis/blood , Liver Cirrhosis/metabolism , Male , Middle Aged , Predictive Value of Tests , Zambia/epidemiologyABSTRACT
The results of observations of 184 children from the CHD after surgical correction on which explored the biochemical markers of myocardial injury in serum--myocardial fraction of creatine kinase and galectin-3. The relationship between increased serum concentrations of these markers, clinical and paraclinical characteristics of examined children.
Subject(s)
Creatine Kinase, MB Form/blood , Galectin 3/blood , Heart Defects, Congenital/blood , Myocardium/metabolism , Adolescent , Biomarkers/blood , Blood Proteins , Child , Child, Preschool , Creatine Kinase, MB Form/genetics , Female , Galectin 3/genetics , Galectins , Gene Expression , Heart Defects, Congenital/genetics , Heart Defects, Congenital/pathology , Heart Defects, Congenital/surgery , Humans , Infant , Male , Myocardium/pathologyABSTRACT
Provided evaluation of the medical and social inspection of the dcotrors-consultative commission of Vinnytsya regional childrens hospital in children with juvenile rheumatoid arthritis (JRA). Patients with JRA that got position of the child-invalid are given individual program of the rehabilitation that includes medical, professional, sport and physical, social adaptation that gives ability control recommendations for both--physicians and parents.
Subject(s)
Arthritis, Juvenile/rehabilitation , Disability Evaluation , Physical Therapy Modalities/psychology , Adolescent , Arthritis, Juvenile/diagnosis , Arthritis, Juvenile/economics , Arthritis, Juvenile/therapy , Child , Humans , Physical Examination , Physical Therapy Modalities/economics , Physician-Patient Relations , Social Adjustment , UkraineABSTRACT
The results of triple Helicobacter-therapy (omeprazole, amoxicillin, nifuratel) in the treatment of chronic gastroduodenal pathology in children depending on the duration of it's use. The effectiveness of drug therapy was evaluated in terms of eradication of Helicobacter pylori and dynamics of pain, dyspeptic syndrome and astenovegetative syndrome.
Subject(s)
Anti-Bacterial Agents/therapeutic use , Duodenitis/drug therapy , Gastritis/drug therapy , Helicobacter Infections/drug therapy , Helicobacter pylori/drug effects , Pain/prevention & control , Adolescent , Amoxicillin/therapeutic use , Antigens, Bacterial/genetics , Antigens, Bacterial/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Child , Chronic Disease , Drug Administration Schedule , Drug Therapy, Combination , Duodenitis/complications , Duodenitis/microbiology , Duodenitis/physiopathology , Gastritis/complications , Gastritis/microbiology , Gastritis/physiopathology , Gene Expression , Helicobacter Infections/complications , Helicobacter Infections/microbiology , Helicobacter Infections/physiopathology , Helicobacter pylori/genetics , Helicobacter pylori/growth & development , Helicobacter pylori/pathogenicity , Humans , Nifuratel/therapeutic use , Omeprazole/therapeutic use , Pain/complications , Pain/microbiology , Pain/physiopathology , Treatment OutcomeABSTRACT
The aim of our work was to determine the the gene polymorphism of IL-1ß in children with chronic glomerulonephritis. there has been defined the frequency of combinations of allelic variants of cytokines genes of IL-1ß and IL-10 in chronic glomerulonephritis in children and found the connection of allelic polymorphism with the disease course, indices of hemopoiesis and IL-1ß in serum. The presence of C/T genotype is associated with increased production of interleukin-1ß in serum. Genetic polymorphism of genes of interleukin can be a new marker of progression of chronic kidney disease.