ABSTRACT
It is now recognized that Acinetobacter spp. play a significant role in the colonization and infection of patients admitted to hospitals. The virulence factors of A. baumannii remains largery unknown. In this study, the adherence of A. baumannii to several species of red blood cells was investigated. The ruthenium red staining was used for electron microscopic studies. The results obtained in electron microscopy and the hemagglutination studies suggested that the thin and long fimbriae of A. baumannii participated in adhesion of these bacteria to red blood cells.
Subject(s)
Acinetobacter/physiology , Bacterial Adhesion/physiology , Erythrocytes/microbiology , Acinetobacter/ultrastructure , Animals , Hemagglutination , Humans , Microscopy, Electron , Rabbits , SheepSubject(s)
Autoantibodies/analysis , Milk Proteins/immunology , Age Factors , Animals , Cattle , Child , Child, Preschool , Female , Growth , Humans , InfantSubject(s)
Cryoglobulins , Occupational Diseases/chemically induced , Paraproteinemias/chemically induced , Raynaud Disease/chemically induced , Vinyl Chloride/poisoning , Vinyl Compounds/poisoning , Adult , Environmental Exposure , Female , Humans , Male , Middle Aged , Occupational Diseases/immunology , Raynaud Disease/immunologyABSTRACT
32P-cyclophosphamide was found to combine with gamma-globulin fractions of immune sera. Immune sera incubated with 32P-cyclophosphamide retained ability to react specifically with homologous antigen in vitro in the system: MN antigens of human erythrocytes + rabbit anti-MN antibody, and probably reacted selectively with target antigens in vivo in the system: antigens of guinea pig kidney tissue + rabbit antibodies against these antigens. Hemagglutination, passive hemagglutination and precipitation in agar gel tests were used in the experiments. Ability to combine of the immune antibody + 32P-cyclophosphamide complex with homologous antigens was evaluated by measurements of radioactivity of studied materials (erythrocyte agglutinates and organ homogenates). The results indicate feasibility of using immune antibodies as carriers of cytostatic agents.
Subject(s)
Alkylating Agents/metabolism , Antigen-Antibody Complex , Isoantibodies , MNSs Blood-Group System , Animals , Antigen-Antibody Reactions , HumansABSTRACT
Sera of 48 rabbits after long-term immunization with suspensions of Diplococcus pneumoniae, Klebsiella friedländeri, Proteus OX19 and solution of bovine albumin were examined for antibodies reacting with homologous gamma-globulins and antigens of thermally denatured autologous sera. Appearance of antibodies of this type was related to duration of immunization, but not related to the type of antigen used. The experimental results were explained as a result of altered antigenic properties of antibodies following reaction with homologous antigen.
Subject(s)
Antibodies, Anti-Idiotypic , Serum Globulins , Agglutinins/analysis , Animals , Antibodies, Anti-Idiotypic/isolation & purification , Antigens, Bacterial , Goats/immunology , Immune Sera , Immunization , Klebsiella/immunology , Protein Denaturation , Proteus/immunology , Rabbits , Serum Albumin, Bovine , Sheep/immunology , Streptococcus pneumoniae/immunology , Temperature , Time FactorsABSTRACT
The autoimmunogenic properties of whole rabbit serum heated at 56 degrees C and 60 degrees C were studied in rabbits. Autologous rabbit serum heated 20 min at 60 degrees C was immunogenic. The state of induced immunity manifested itself by the appearance of antibodies to antigens of heterologous sera: human, goat and guinea pig. No antibodies for autologous sera were found, including autologous sera heated 20 min at 60 degrees C. The antibodies for heterologous sera directed against gamma-globulins were detected by the passive hemagglutination test and by precipitation in agar gel.
Subject(s)
Antibodies, Anti-Idiotypic/analysis , Antibody Formation , Antigens , Animals , Autoantibodies , Cross Reactions , Female , Goats/immunology , Guinea Pigs/immunology , Hemagglutination Tests , Immune Sera , Immunodiffusion , Immunoelectrophoresis , Male , Rabbits , Species Specificity , Temperature , Time FactorsABSTRACT
Sera from 14 of 50 patients suffering from bronchial asthma complicated by suppurative catarrh of the respiratory tract contained antibodies to homologous and heterologous gamma-globulins. The findings were attributed to hyperimmunization causing changed in antigenic properties of antibodies. Antigenically altered antibodies can act as autoimmunogenic stimuli inducing an immune response manifested by the appearance of antibodies to autologous gamma-globulins and cross reacting with homologous and heterologous gamma-globulins.
Subject(s)
Antibodies, Anti-Idiotypic , Antibody Specificity , Asthma/immunology , Respiratory Tract Infections/immunology , Adult , Aged , Cross Reactions , Female , Humans , Male , Middle Aged , Suppuration/immunology , gamma-GlobulinsABSTRACT
Electrophorograms of human and rabbit sera before and after incubation with various concentrations of cyclophosphamide and chlormethine (Nitrogranulogen) were compared. Sera of rabbits injected with chlormethine and cyclophosphamide in therapeutic doses for humans were examined for antibodies reacting with homologous and antologous gamma-globulins. Immunoelectrophorograms of sera incubated with both alkylating agents differed from those of preincubation sera with regard to number and intensity of precipitin lines. The changes in electrophorograms were related to the type and concentration of alkylating agent. No antibodies to homologous or autologous serum antigens of rabbits treated with chlormethine or cyclophosphamide were found.
