Barriers and Facilitators to COVID-19 Vaccine Uptake among Polish Patients: A Qualitative Interview Study.

The decision to receive a COVID-19 vaccine is influenced by a variety of individual and contextual factors. However, there are very few studies that analyse individual vaccination decisions using a qualitative methodology. To close this gap, we conducted a qualitative interview study to examine the opinions and experiences with the vaccine among patients previously hospitalized due to COVID-19, including barriers and facilitators to vaccine uptake. An exploratory qualitative study, using semi-structured telephone interviews, was conducted among 22 patients admitted for COVID-19 in Poland in 2022. Opinions of patients previously hospitalized with COVID-19 on vaccination were varied. Barriers to COVID-19 vaccine uptake stemmed from concerns about vaccine safety, patients' religious beliefs, and negative stories. High disease severity and anxiety over personal and family health were important arguments in favour of receiving the COVID-19 vaccine. The study findings indicated the need for ongoing health education by healthcare staff as well as coordination and integration of multi-sectoral institutional measures regarding COVID-19 prevention strategies as well as increased public health initiatives on social media and engagement of community leaders for awareness about vaccines and vaccination. It is crucial to build trust in COVID-19 vaccinations among the general public by disseminating reliable information through trustworthy and credible sources. However, it ought to be emphasised that, regardless of the measures taken, some individuals will remain unconvinced about receiving a COVID-19 vaccine.

The Application of a Hybrid Method for the Identification of Elastic-Plastic Material Parameters.

The indentation test is a popular method for the investigation of the mechanical properties of materials. The technique, which combines traditional indentation tests with mapping the shape of the imprint, provides more data describing the material parameters. In this paper, such methodology is employed for estimating the selected material parameters described by Ramberg-Osgood's law, i.e., Young's modulus, the yield point, and the material hardening exponent. Two combined identification methods were used: the P-A procedure, in which the material parameters are identified on the basis of the coordinates of the indentation curves, and the P-C procedure, which uses the coordinates describing the imprint profile. The inverse problem was solved by neural networks. The results of numerical indentation tests-pairs of coordinates describing the indentation curves and imprint profiles-were used as input data for the networks. In order to reduce the size of the input vector, a simple and effective method of approximating the branches of the curves was proposed. In the Results Section, we show the performance of the approximation as a data reduction mechanism on a synthetic dataset. The sparse model generated by the presented approach is also shown to efficiently reconstruct the data while minimizing error in the prediction of the mentioned material parameters. Our approach appeared to consistently provide better performance on the testing datasets with considerably easier computation than the principal component analysis compression results available in the literature.

Behavioural categories of professionalism of nurses in Poland and Belarus: A comparative survey.

AIM AND OBJECTIVES: To compare the self-reported level of professionalism among nurses in Poland and Belarus and to indicate the areas in which differences in professional behaviours of nurses in both countries exist. BACKGROUND: Nurses constitute the largest group of healthcare providers, and the term professionalism is closely related to nursing profession. DESIGN: This investigation is a comparative survey and descriptive analysis of professional behaviours among nurses in Poland (n = 205) and Belarus (n = 236). The study was reported according to the STROBE checklist. METHODS: The Professionalism in Nursing Behaviors' Inventory Image Survey adapted from Adams and Miller (2001) was used to collect the data. The questionnaire contains 46 questions addressing the following behavioural categories: educational preparation, publications, research, professional organisation, community service, competence, code for nurses, theory and autonomy. RESULTS: The mean total score of professionalism was significantly different between the two countries (p < .0001). Significant differences, with higher scores in the group of respondents from Belarus, were also revealed in the following subscales of professionalism: "Professional organisation," "Community service," "Competence" and "Publication." Nurses from Poland had higher levels of "Educational preparation" and "Use of theory." No statistically significant differences were indicated in the "Autonomy" and "Research" subscales. CONCLUSION: Differences in the professionalism level of nurse practitioners in Poland and Belarus are related to nurses' professional position and the context in which they practice. RELEVANCE TO CLINICAL PRACTICE: The results of the present study can help the healthcare management and nursing leaders to support the professional development of nurses and strive for a higher level of professionalism.

Chemoenzymatic Synthesis of Proxyphylline Enantiomers.

A novel synthetic route for preparation of proxyphylline enantiomers using a kinetic resolution (KR) procedure as the key step is presented. The reactions were catalyzed by immobilized Candida antarctica lipase B in acetonitrile. Three types of reactions were examined: (i) enantioselective transesterification of racemic proxyphylline with vinyl acetate as well as (ii) hydrolysis and (iii) methanolysis of its esters. The influence of reaction conditions on the substrate conversion and enantiomeric purity of the products were investigated. Studies on analytical scale reactions revealed that the titled API enantiomers could be successfully obtained with excellent enantiomeric excess (up to >99% ee). The process was easily conducted on a 5 g scale at 100 g/L. In a preparative-scale reaction, unreacted (S)-(+)-butanoate (97% ee) and (R)-(-)-alcohol (96% ee) were obtained after 2 days in yields of 45% and 46%, respectively. When the reaction time was extended to 6 days, (S)-(+)-butanoate was isolated in >99% ee and acceptable high enantioselectivity (E = 90). Importantly, the KR's products could be conveniently isolated by exploiting varying solubility of the ester/alcohol in acetonitrile at room temperature. In addition, a chiral preference of the CAL-B active site for the R-enantiomer was rationalized by in sillico docking studies.

Mechanistic basis for the enantioselectivity of the anaerobic hydroxylation of alkylaromatic compounds by ethylbenzene dehydrogenase.

The enantioselectivity of reactions catalyzed by ethylbenzene dehydrogenase, a molybdenum enzyme that catalyzes the oxygen-independent hydroxylation of many alkylaromatic and alkylheterocyclic compounds to secondary alcohols, was studied by chiral chromatography and theoretical modeling. Chromatographic analyses of 22 substrates revealed that this enzyme exhibits remarkably high reaction enantioselectivity toward (S)-secondary alcohols (18 substrates converted with >99% ee). Theoretical QM:MM modeling was used to elucidate the structure of the catalytically active form of the enzyme and to study the reaction mechanism and factors determining its high degree of enantioselectivity. This analysis showed that the enzyme imposes strong stereoselectivity on the reaction by discriminating the hydrogen atom abstracted from the substrate. Activation of the pro(S) hydrogen atom was calculated to be 500 times faster than of the pro(R) hydrogen atom. The actual hydroxylation step (i.e., hydroxyl group rebound reaction to a carbocation intermediate) does not appear to be enantioselective enough to explain the experimental data (the calculated rate ratios were in the range of only 2-50 for pro(S): pro(R)-oriented OH rebound).

BN/CC isosteric compounds as enzyme inhibitors: N- and B-ethyl-1,2-azaborine inhibit ethylbenzene hydroxylation as nonconvertible substrate analogues.

Good substrate gone bad! BN/CC isosterism of ethylbenzene leads to N-ethyl-1,2-azaborine and B-ethyl-1,2-azaborine. In contrast to ethylbenzene, which is the substrate for ethylbenzene dehydrogenase (EbDH), N-ethyl-1,2-azaborine (see scheme; Fc=Ferricenium tetrafluoroborate) and B-ethyl-1,2-azaborine are strong inhibitors of EbDH. Thus, the changes provided by BN/CC isosterism can lead to new biochemical reactivity.

Substrate and inhibitor spectra of ethylbenzene dehydrogenase: perspectives on application potential and catalytic mechanism.

Ethylbenzene dehydrogenase (EbDH) catalyzes the initial step in anaerobic degradation of ethylbenzene in denitrifying bacteria, namely, the oxygen-independent hydroxylation of ethylbenzene to (S)-1-phenylethanol. In our study we investigate the kinetic properties of 46 substrate analogs acting as substrates or inhibitors of the enzyme. The apparent kinetic parameters of these compounds give important insights into the function of the enzyme and are consistent with the predicted catalytic mechanism based on a quantum chemical calculation model. In particular, the existence of the proposed substrate-derived radical and carbocation intermediates is substantiated by the formation of alternative dehydrogenated and hydroxylated products from some substrates, which can be regarded as mechanistic models. In addition, these results also show the surprisingly high diversity of EbDH in hydroxylating different kinds of alkylaromatic and heterocyclic compounds to the respective alcohols. This may lead to attractive industrial applications of ethylbenzene dehydrogenase for a new process of producing alcohols via hydroxylation of the corresponding aromatic hydrocarbons rather than the customary procedure of reducing the corresponding ketones.

Quantitative structure enantioselective retention relationship for high-performance liquid chromatography chiral separation of 1-phenylethanol derivatives.

The Quantitative Structure Retention Relationship (QSRR) modeling techniques are employed for prediction of retention behavior of chiral secondary alkylaromatic and alkylheterocyclic alcohols, derivatives of 1-phenylethanol, separated on Chiracel OB-H column. Genetic algorithms and neural networks are used to obtain models predicting Retention Order Index (ROI) (R(2) - 0.99), selectivity ROI log alpha (R(2) - 0.93) as well as retention factors (log k) for two types of mobile phases (90/10 and 85/15 n-hexane/isopropanol--R(2) - 0.97 and 0.95). Additionally, a model that predicts log k for both mobile phase in function of i-PrOH concentration is developed (R(2) - 0.97). HOMO energy turns out to be the most important parameter in description of log k while mixed steric-electrostatic interactions with chiral OH group and furan ring are responsible for the chiral recognition. The models are used to assess the stereoselectivity of ethylbenzene dehydrogenase (EBDH), which catalyzes stereospecific syntheses of the investigated compounds. The high stereoselectivity of the enzyme is confirmed but reversion of EBDH enantioselectivity is predicted to take place in the biosynthesis of 1-[1,1'-biphenyl]-4-ylethanol.