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1.
Clin Vaccine Immunol ; 19(7): 1019-26, 2012 Jul.
Article in English | MEDLINE | ID: mdl-22593238

ABSTRACT

The parasitic nematode Parelaphostrongylus tenuis is an important cause of neurologic disease of camelids in central and eastern North America. The aim of this study was to determine whether alpacas develop resistance to disease caused by P. tenuis in response to a previous infection or a combination of controlled infection and immunization. Alpacas were immunized with a homogenate of third-stage larvae (L3) and simultaneously implanted subcutaneously with diffusion chambers containing 20 live L3. Sham-treated animals received adjuvant alone and empty chambers. The protocol was not effective in inducing resistance to oral challenge with 10 L3, and disease developed between 60 and 71 days following infection. Immediately following the onset of neurologic disease, affected animals were treated with a regimen of anthelmintic and anti-inflammatory drugs, and all recovered. One year later, a subset of alpacas from this experiment was challenged with 20 L3 and the results showed that prior infection induced resistance to disease. Primary and secondary infections induced production of conventional and heavy-chain IgGs that reacted with soluble antigens in L3 homogenates but did not consistently recognize a recombinant form of a parasite-derived aspartyl protease inhibitor. Thus, the latter antigen may not be a good candidate for serology-based diagnostic tests. Antibody responses to parasite antigens occurred in the absence of overt disease, demonstrating that P. tenuis infection can be subclinical in a host that has been considered to be highly susceptible to disease. The potential for immunoprophylaxis to be effective in preventing disease caused by P. tenuis was supported by evidence of resistance to reinfection.


Subject(s)
Antibodies, Helminth/blood , Metastrongyloidea/immunology , Strongylida Infections/immunology , Adjuvants, Immunologic/administration & dosage , Animals , Anthelmintics/administration & dosage , Anti-Inflammatory Agents/administration & dosage , Antigens, Helminth/administration & dosage , Antigens, Helminth/immunology , Camelids, New World , Male , Strongylida Infections/drug therapy , Strongylida Infections/prevention & control , Treatment Outcome , Vaccination/methods
2.
Vet Pathol ; 44(1): 119-22, 2007 Jan.
Article in English | MEDLINE | ID: mdl-17197637

ABSTRACT

Parasitic granulomatous eosinophilic inflammation was observed in the central nervous system (CNS) of a 6-month-old Arabian colt from New York state. Inflammation was associated with eggs, larvae, and adult nematodes in the cerebellum. Nematodes had histological characteristics of the superfamily Metastrongyloidea. The presence of dorsal-spined larvae in the CNS was further indicative of infection with a nematode in the family Protostrongylidae. Infections were most compatible with Parelaphostrongylus tenuis but specific diagnosis was not possible. This is the first definitive report of a protostrongylid nematode infection in a horse.


Subject(s)
Encephalitis/veterinary , Horse Diseases/parasitology , Metastrongyloidea/growth & development , Strongylida Infections/veterinary , Animals , Cerebellum/parasitology , Cerebellum/pathology , Encephalitis/parasitology , Encephalitis/pathology , Fatal Outcome , Female , Histocytochemistry/veterinary , Horse Diseases/pathology , Horses , Male , Strongylida Infections/parasitology , Strongylida Infections/pathology
3.
Clin Diagn Lab Immunol ; 12(3): 380-6, 2005 Mar.
Article in English | MEDLINE | ID: mdl-15753251

ABSTRACT

Of the three immunoglobulin G (IgG) isotypes described to occur in camelids, IgG2 and IgG3 are distinct in that they do not incorporate light chains. These heavy-chain antibodies (HCAbs) constitute approximately 50% of the IgG in llama serum and as much as 75% of the IgG in camel serum. We have produced isotype-specific mouse monoclonal antibodies (MAbs) in order to investigate the roles of HCAbs in camelid immunity. Seventeen stable hybridomas were cloned, and three MAbs that were specific for epitopes on the gamma chains of llama IgG1, IgG2, or IgG3 were characterized in detail. Affinity chromatography revealed that each MAb bound its isotype in solution in llama serum. The antibodies bound to the corresponding alpaca IgGs, to guanaco IgG1 and IgG2, and to camel IgG1. Interestingly, anti-IgG2 MAbs bound three heavy-chain species in llama serum, confirming the presence of three IgG2 subisotypes. Two IgG2 subisotypes were detected in alpaca and guanaco sera. The MAbs detected llama serum IgGs when they were bound to antigen in enzyme-linked immunosorbent assays and were used to discern among isotypes induced during infection with a parasitic nematode. Diseased animals, infected with Parelaphostrongylus tenuis, did not produce antigen-specific HCAbs; rather, they produced the conventional isotype, IgG1, exclusively. Our data document the utility of these MAbs in functional and physiologic investigations of the immune systems of New World camelids.


Subject(s)
Antibodies, Monoclonal , Camelids, New World/immunology , Immunoglobulin Heavy Chains/immunology , Animals , Antibodies, Monoclonal/biosynthesis , Chromatography, Affinity , Enzyme-Linked Immunosorbent Assay , Epitopes , Immunoglobulin G/immunology , Immunoglobulin Isotypes/immunology , Mice , Mice, Inbred BALB C , Nematode Infections/immunology
4.
Nurs Clin North Am ; 36(3): 603-15, xii, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11532673

ABSTRACT

The development of gynecologic oncology as a specialty has contributed to the progress of the treatment and management of women with genital and reproductive cancers. The trend toward less aggressive and less invasive surgery has yielded an improved quality of life for women with gynecologic cancer. Multimodal interdisciplinary care is the principle and practice of gynecologic oncology. Prevention and screening have not always kept stride with the other advances in treatment, which include diagnostic imaging, laparoscopic surgery, chemotherapy, genetic susceptibility, and tumor marker assays. Educating patients and health care providers is the challenge in gynecologic oncology.


Subject(s)
Genital Neoplasms, Female/surgery , Combined Modality Therapy , Female , Genital Neoplasms, Female/diagnosis , Genital Neoplasms, Female/epidemiology , Genital Neoplasms, Female/psychology , Gynecologic Surgical Procedures/methods , Gynecologic Surgical Procedures/trends , Humans , Laparoscopy/methods , Laparoscopy/trends , Mass Screening/methods , Medical Oncology/methods , Medical Oncology/trends , Neoplasm Staging/methods , Patient Care Team/organization & administration , Primary Prevention/methods , Quality of Life , Survival Analysis , Treatment Outcome
5.
J Wildl Dis ; 35(4): 783-5, 1999 Oct.
Article in English | MEDLINE | ID: mdl-10574541

ABSTRACT

The potential of protostrongylid first-stage larvae (L1) to survive passage through the alimentary canal of non-infected mammals was investigated. Parelaphostrongylus tenuis L1 were collected from feces of an experimentally infected white-tailed deer (Odocoileus virginianus). We utilized two red deer (Cervus elaphus) and four laboratory rats (Rattus norvegicus) which were each fed the L1 of P. tenuis. Larvae were recovered, intact and alive, from the fecal samples of all six animals. Larvae of P. tenuis, and probably of other related species, can survive passage through the alimentary canal of uninfected mammals and they can be collected from feces using the Baermann technique and other related larval extraction methods. Rain water was found to be successful in the dispersal of P. tenuis L1 from the feces of infected animals. These findings raise the possibility of ingestion of L1 and their subsequent passage, by uninfected animals. This potential for false-positive diagnosis of infection in live animals necessitates accurate interpretation of a host's infection-status. Such findings reinforce the need for a reliable method of diagnosing infections in live animals.


Subject(s)
Deer/parasitology , Metastrongyloidea/isolation & purification , Strongylida Infections/veterinary , Animals , Digestive System/parasitology , False Positive Reactions , Feces/parasitology , Larva/growth & development , Rain , Rats , Strongylida Infections/diagnosis
6.
N Z Vet J ; 46(5): 182-5, 1998 Oct.
Article in English | MEDLINE | ID: mdl-16032046

ABSTRACT

AIMS: To measure the efficacy of a pour-on formulation of moxidectin against lungworm and abomasal parasites in weaner wapiti x red deer and to compare this with its efficacy in weaner red deer. METHODS: Six red and six wapiti hybrid deer, naturally infected with lungworm and gastro-intestinal parasites, were treated with pour-on moxidectin at 500 microg/kg body weight and slaughtered 14 or 16 days later, along with six red and six wapiti hybrid untreated control deer. Total worm counts were performed on the lungs, abomasum and abomasal digest of each deer. RESULTS: The efficacy of moxidectin pour-on was 100% against adult and immature lungworms (Dictyocaulus viviparus) in red deer, and 100% and 99.7% effective against adult and immature lungworm in wapiti hybrid deer. The efficacy of moxidectin pour-on was 100, 100, 99.9 and 99.9% respectively against adult, fifth stage, late fourth stage and early fourth stage larvae of Ostertagia-type nematodes (assumed to be Ostertagia, Spiculopteragia, Skrjabinagia and Apteragia spp.) in both red and wapiti hybrid deer. CONCLUSIONS: The pour-on formulation of moxidectin, at 500 microg/kg body weight, is highly effective against mature and immature lungworms and abomasal nematodes in wapiti hybrid deer and equally effective in red deer.

7.
J Parasitol ; 80(3): 478-80, 1994 Jun.
Article in English | MEDLINE | ID: mdl-8195953

ABSTRACT

Between May 1989 and October 1991, 544 black bears Ursus americanus were collected in New Brunswick and examined for Trichinella spiralis larvae. In addition, 110 of these bears were examined for filarial nematodes, and the entire intestinal tracts of 12 bears were examined for intestinal helminths. Four species of helminths were identified from these bears, including Trichinella spiralis, Dirofilaria ursi, Baylisascaris transfuga, and Taenia krabbei. This represents the first documentation of T. spiralis in Maritime Canada and extends eastward the range of T. krabbei.


Subject(s)
Helminthiasis, Animal , Ursidae/parasitology , Animals , Ascaridida Infections/epidemiology , Ascaridida Infections/parasitology , Ascaridida Infections/veterinary , Ascaridoidea/isolation & purification , Dirofilaria/isolation & purification , Dirofilariasis/epidemiology , Dirofilariasis/parasitology , Helminthiasis/epidemiology , Helminthiasis/parasitology , Intestinal Diseases, Parasitic/epidemiology , Intestinal Diseases, Parasitic/parasitology , Intestinal Diseases, Parasitic/veterinary , Male , New Brunswick/epidemiology , Prevalence , Taenia/isolation & purification , Taeniasis/epidemiology , Taeniasis/parasitology , Taeniasis/veterinary , Tongue/parasitology , Trichinella spiralis/isolation & purification , Trichinellosis/epidemiology , Trichinellosis/parasitology , Trichinellosis/veterinary
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