Subject(s)
Aldehydes , Amines , Meat , Nitrosamines , Chemical Phenomena , Chemistry , Freeze Drying , Imines , NitritesSubject(s)
Linoleic Acids , Muscle Proteins , Salmon , Amino Acids/analysis , Animals , Chemical Phenomena , Chemistry , Chromatography, Thin Layer , Fish Products/analysis , Linoleic Acids/analysis , Lipids/analysis , Myosins/analysis , Oxidation-Reduction , Oxygen Consumption , Protein Denaturation , Spectrometry, Fluorescence , Spectrophotometry, InfraredSubject(s)
Dieldrin/analysis , Milk/analysis , Solvents , Adsorption , Animals , Blood Proteins/analysis , Butter/analysis , Ethanol , Lipids/analysis , Methods , Milk Proteins/analysis , Oxalates , Solvents/classificationSubject(s)
Phosphatidylcholines/metabolism , Phosphatidylethanolamines/metabolism , Amines/pharmacology , Egg Yolk/analysis , Emulsions , Female , Methods , Nitrogen/metabolism , Oxygen Consumption , Phosphatidylcholines/isolation & purification , Phosphatidylethanolamines/isolation & purification , Glycine max/analysisSubject(s)
Lipase , Animals , Chemical Phenomena , Chemistry , Fatty Acids , Hot Temperature , Oils , Palmitic Acids , Pancreas/enzymology , Swine , Time Factors , Triglycerides , VibrationSubject(s)
Collagen , Connective Tissue , Muscles , Skin , Age Factors , Animals , Cattle , Collagen/metabolism , Connective Tissue/metabolism , Deficiency Diseases , Skin/metabolism , Swine , Temperature , ZincSubject(s)
Dietary Fats , Food Preservation , Nutritional Physiological Phenomena , Animals , Chickens , Dietary Fats/adverse effects , Fats, Unsaturated , Food-Processing Industry , Hot Temperature , Lipids/toxicity , Mice , Oxidation-Reduction , Peroxides/metabolism , Peroxides/toxicity , Polymers , Rats , StereoisomerismABSTRACT
A method for the quantitative determination of plasmalogens is described. Glycerophosphatide fractions from animal and plant sources were treated with a 2,4-dinitrophenylhydrazine-phosphoric acid reagent. The plasmalogen content was estimated by two different procedures after a single treatment with reagent: a) by determining the phosphorus content of diacyl phospholipid and (2-acyl) lysophospholipid separated by thin-layer chromatography, b) by spectrophotometric determination of the 2,4-dinitrophenylhydrazones and phosphorus assay of the original untreated sample. The total plasmalogen content of an unfractionated extract may be determined easily by the latter procedure.The method described gives results comparable with those from the p-nitrophenylhydrazine method and iodination procedure. The usefulness of this 2,4-dinitrophenylhydrazine-phosphoric acid treatment for further structural analyses of plasmalogens is discussed.
ABSTRACT
The procedure for low temperature methylation of fatty acids in lipids by sulfuric acid-methanol has been adapted to direct methylation of fatty acids of lipids in biological materials without prior extraction of the lipids. Successful application requires a solution or a suspension of fine particles of the lipid bearing material in ether. Concentrated sulfuric acid is added to the solution or suspension at low temperatures followed by addition of absolute methanol. The acid is neutralized by methanolic KOH and the esters extracted. Application of the method to prepare methyl esters of lipids in cream, blood serum, swine liver and kidney tissue, and cells of yeast onStaphylococcus aureus show that fatty acid composition based on this method compares with that determined by methylation of extracted lipids.
ABSTRACT
Isopolyunsaturated lipids isolated from plant and animal sources were included in the diets of mature male rats. Liver and blood serum cholesterol lowering effects were noted only in the lipid from the vegetable source. The authors suggest that the cholesterol lowering effect of vegetable oils is associated with the generally betaunsaturated triglycerides found therein.
