ABSTRACT
The original set of 12 clones of hybridomas, producers of monoclonal antibodies (mAbs) against human IgE, has been obtained. The study of biological properties of antibodies has been conducted: their specificity, affinity constant and titer in a cultural medium have been established. The obtained mAbs are directed to the two epitope regions on IgE molecule. The first group of mAbs relates to epitope region, represented by three epitopes (two of them overlap and one that does not overlap with others). The second epitope region is represented by only one epitope.
Subject(s)
Antibodies, Monoclonal/isolation & purification , Epitopes/immunology , Immunoglobulin E/immunology , Antibodies, Monoclonal/biosynthesis , Antibody Affinity , Antibody Specificity , Clone Cells , Enzyme-Linked Immunosorbent Assay , Epitope Mapping , Humans , Hybridomas/cytology , Hybridomas/immunology , Lymphocytes/cytology , Lymphocytes/immunologyABSTRACT
Using the Ames semiquantitative technique with metabolic activation by the postmitochondrial fraction of rat liver homogenate preprocessed by phenobarbital sodium salt (for induction of monooxygenases of mixed function) potential mutagenic activity of 17 colouring balms which are used as cosmetic products for hair dyeing has been studied. The balms include water soluble dyes and pigments depositing on the surface of the hair. Potential mutagenic activity of almost all investigated colouring products has been shown. Three compositions (red tree, chestnut and black) have appeared to be the most active inductors of gene mutations showing the effect of "mean force" according to our classification. One of balms (burgund) has appeared as genetically inert. The rest balms have shown mutagen effect of "gentle force" with the ratio of control value excess from 2 up to 5-fold.
Subject(s)
Hair Dyes/pharmacology , Mixed Function Oxygenases/biosynthesis , Mutagenicity Tests/methods , Salmonella typhimurium/drug effects , Animals , Enzyme Induction/drug effects , Hair Dyes/chemistry , Liver/enzymology , Liver/metabolism , Microsomes, Liver/drug effects , Microsomes, Liver/enzymology , Mutagens/pharmacology , Organisms, Genetically Modified , Phenobarbital/pharmacology , Rats , Salmonella typhimurium/genetics , Structure-Activity RelationshipABSTRACT
The results of ecogenetical investigations of air, water, and soil are presented. A possibility of ranging territories by the indices of environmental mutagenicity and their significance for genetic monitoring are demonstrated.
Subject(s)
Ecology , Environmental Monitoring , Genetic Testing , Air/analysis , Benzo(a)pyrene/chemistry , Humans , Soil/analysis , Ukraine , Water/analysisABSTRACT
Antimutagenic activity of 20 and 40% ethanol extracts from the biomass of Rhodiola rosea, Polyscias filicifolia, Panax ginseng and Ungernia victoris cultured cells have been studied. DDDTDP, ethidium bromide, benz(a)pyrene, benzidine served as model mutagens for Salmonella typhimurium TA 98 strain (the latter two were tested in presence of metabolic activation system); for S. typhimurium TA 100 strain these were tio-tefa, bichromate potassium and sodium azide and heavy metal compounds (chlorides of manganese, zinc, cadmium, lead acetate) for both strains. Higher capacity of the extracts from the biomass of R. rosea and P. filicifolia to counteract gene mutations induced by various mutagens was demonstrated (ca. 90% inhibition in isolated cases). In the experiment with the metabolic activation most effective proved to be the extracts from the P. ginseng biomass (up to 34% and 47% mutagenicity inhibition).
