ABSTRACT
Dengue fever, a mosquito-borne viral disease of significant public health concern in tropical and subtropical regions, is caused by any of the four serotypes of the dengue virus (DENV1-4). Cutting-edge technologies like next-generation sequencing (NGS) are revolutionizing virology, enabling in-depth exploration of DENV's genetic diversity. Here, we present an optimized workflow for full-genome sequencing of DENV 1-4 utilizing tiled amplicon multiplex PCR and Illumina sequencing. Our assay, sequenced on the Illumina MiSeq platform, demonstrates its ability to recover the full-length dengue genome across various viral abundances in clinical specimens with high-quality base coverage. This high quality underscores its suitability for precise examination of intra-host diversity, enriching our understanding of viral evolution and holding potential for improved diagnostic and intervention strategies in regions facing dengue outbreaks.
Subject(s)
Dengue Virus , Dengue , Genome, Viral , High-Throughput Nucleotide Sequencing , Multiplex Polymerase Chain Reaction , Serogroup , Whole Genome Sequencing , Dengue Virus/genetics , Dengue Virus/classification , Dengue Virus/isolation & purification , Multiplex Polymerase Chain Reaction/methods , Dengue/virology , Dengue/diagnosis , Humans , Genome, Viral/genetics , Whole Genome Sequencing/methods , High-Throughput Nucleotide Sequencing/methods , RNA, Viral/geneticsABSTRACT
Dengue is the most common arboviral infection of humans and is a public health burden in more than 100 countries. Aedes aegypti mosquitoes stably infected with strains of the intracellular bacterium Wolbachia are resistant to dengue virus (DENV) infection and are being tested in field trials. To mimic field conditions, we experimentally assessed the vector competence of A. aegypti carrying the Wolbachia strains wMel and wMelPop after challenge with viremic blood from dengue patients. We found that wMelPop conferred strong resistance to DENV infection of mosquito abdomen tissue and largely prevented disseminated infection. wMel conferred less resistance to infection of mosquito abdomen tissue, but it did reduce the prevalence of mosquitoes with infectious saliva. A mathematical model of DENV transmission incorporating the dynamics of viral infection in humans and mosquitoes was fitted to the data collected. Model predictions suggested that wMel would reduce the basic reproduction number, R0, of DENV transmission by 66 to 75%. Our results suggest that establishment of wMelPop-infected A. aegypti at a high frequency in a dengue-endemic setting would result in the complete abatement of DENV transmission. Establishment of wMel-infected A. aegypti is also predicted to have a substantial effect on transmission that would be sufficient to eliminate dengue in low or moderate transmission settings but may be insufficient to achieve complete control in settings where R0 is high. These findings develop a framework for selecting Wolbachia strains for field releases and for calculating their likely impact.
Subject(s)
Aedes/microbiology , Aedes/virology , Dengue Virus/physiology , Dengue/microbiology , Dengue/virology , Wolbachia/physiology , Adolescent , Adult , Animals , Dengue/transmission , Disease Vectors , Female , Humans , Male , Models, Theoretical , Prospective Studies , Saliva/microbiology , Saliva/virology , Young AdultABSTRACT
Ivermectin has been shown in in vitro studies to have insecticidal properties against Aedes aegypti adults. This study aimed to assess these properties in vivo. Aedes aegypti survival was not affected by acquiring a blood meal from humans both 5 hours and 24 hours after ingestion of a typical dose of ivermectin.
Subject(s)
Aedes/drug effects , Antiparasitic Agents/pharmacology , Insect Vectors/drug effects , Ivermectin/pharmacology , Animals , Female , HumansABSTRACT
Dengue is the most prevalent arboviral disease of humans. The host and virus variables associated with dengue virus (DENV) transmission from symptomatic dengue cases (n = 208) to Aedes aegypti mosquitoes during 407 independent exposure events was defined. The 50% mosquito infectious dose for each of DENV-1-4 ranged from 6.29 to 7.52 log10 RNA copies/mL of plasma. Increasing day of illness, declining viremia, and rising antibody titers were independently associated with reduced risk of DENV transmission. High early DENV plasma viremia levels in patients were a marker of the duration of human infectiousness, and blood meals containing high concentrations of DENV were positively associated with the prevalence of infectious mosquitoes 14 d after blood feeding. Ambulatory dengue cases had lower viremia levels compared with hospitalized dengue cases but nonetheless at levels predicted to be infectious to mosquitoes. These data define serotype-specific viremia levels that vaccines or drugs must inhibit to prevent DENV transmission.
