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1.
Chem Commun (Camb) ; 51(80): 14881-4, 2015 Oct 14.
Article in English | MEDLINE | ID: mdl-26303028

ABSTRACT

Triphenylamines are on/off fluorescent DNA minor groove binders, allowing nuclear staining of fixed cells. By contrast, they accumulate in the cytoplasm of living cells and efficiently trigger cell apoptosis upon prolonged visible light irradiation. This process occurs concomitantly with their subcellular re-localization to the nucleus, enabling fluorescence imaging of apoptosis.


Subject(s)
Aniline Compounds/pharmacology , Cell Death , Cations , Cell Line, Tumor , Humans
2.
Biochimie ; 93(8): 1209-18, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21672604

ABSTRACT

Herein we report on the synthesis and DNA recognition properties of a series of three N-phenyl carbazole-based light-up probes initially designed for two-photon absorption. The vinylic derivatives (Cbz-2Py, Cbz-3Py) display strong fluorescence enhancement when bound to various duplex- and quadruplex-forming oligonucleotides whereas the oxazole derivative is not fluorescent in DNA. Determination of affinity constants by fluorimetric titrations evidenced that Cbz-2Py has a clear preference for AT-rich duplex structures. Circular Dichroism (CD) measurements confirmed the sequence-dependent binding of this compound and suggest insertion in the minor groove as shown by a strong induced CD (ICD) signal and further supported by molecular modeling. Altogether the data indicate that duplex vs quadruplex selectivity of the dyes is strongly dependent on the sequence of the duplex. Finally, the dyes exhibit high two-photon absorption cross-sections (up to 540GM in glycerol) and allow a fine and bright staining of nuclear DNA with low background fluorescence as shown by one and two-photon confocal microscopy imaging of fixed cells.


Subject(s)
Carbazoles/chemistry , DNA/chemistry , Fluorescent Dyes/chemistry , Oligonucleotides/chemistry , Binding Sites , Circular Dichroism , DNA/metabolism , Fluorescent Dyes/chemical synthesis , Fluorescent Dyes/metabolism , Fluorometry , Glycerol/chemistry , Microscopy, Confocal , Models, Molecular , Oligonucleotides/metabolism , Optics and Photonics , Spectrometry, Fluorescence
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