ABSTRACT
Venlafaxine, a second-generation antidepressant, acts by inhibition of the reuptake of presynaptic noradrenaline and serotonin. The main metabolite, O-desmethylvenlafaxine was found biologically active. For toxicological purpose, a rapid specific and accurate RP-HPLC assay was developed for the simultaneous determination of venlafaxine and O-desmethylvenlafaxine in human plasma. A linear response was observed over the concentration range 0.2-4 microg/ml. A good accuracy (<8%) was achieved for all quality controls, with intra-day and inter-day variation coefficient less than 10%. Finally, no interference was observed with other psychotic drugs encountered in acute poisoning. This rapid method (run time <10 min) was used to manage four voluntary intoxications involving venlafaxine.
Subject(s)
Antidepressive Agents, Second-Generation/blood , Chromatography, High Pressure Liquid/methods , Cyclohexanols/blood , Selective Serotonin Reuptake Inhibitors/blood , Antidepressive Agents, Second-Generation/poisoning , Cyclohexanols/poisoning , Desvenlafaxine Succinate , Humans , Reproducibility of Results , Sensitivity and Specificity , Selective Serotonin Reuptake Inhibitors/poisoning , Spectrophotometry, Ultraviolet , Venlafaxine HydrochlorideSubject(s)
Calcitonin/isolation & purification , Hypocalcemia , Animals , Chromatography, Ion Exchange , Methods , Rats , Resins, PlantSubject(s)
Calcitonin/physiology , Parathyroid Hormone/physiology , Animals , Male , Parathyroid Glands/physiology , RatsABSTRACT
Thyrocalcitonin has been suspected of containing iodine, because its analytical behavior and method of extraction are similar to that of iodinated peptides. The iodine content of increasingly pure thyrocalcitonin extracts was determined by two methods, Kolthoff's procedure and activation analysis, with good agreement. A possible link between calcium and iodine metabolism is suggested by these findings.
